Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4AMV

E.COLI GLUCOSAMINE-6P SYNTHASE IN COMPLEX WITH FRUCTOSE-6P

Replaces: 2BPL

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004360	molecular_function	glutamine-fructose-6-phosphate transaminase (isomerizing) activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005975	biological_process	carbohydrate metabolic process
A	0006002	biological_process	fructose 6-phosphate metabolic process
A	0006047	biological_process	UDP-N-acetylglucosamine metabolic process
A	0006048	biological_process	UDP-N-acetylglucosamine biosynthetic process
A	0006487	biological_process	protein N-linked glycosylation
A	0008483	molecular_function	transaminase activity
A	0016740	molecular_function	transferase activity
A	0046349	biological_process	amino sugar biosynthetic process
A	0097367	molecular_function	carbohydrate derivative binding
A	1901135	biological_process	carbohydrate derivative metabolic process
A	1901137	biological_process	carbohydrate derivative biosynthetic process
B	0004360	molecular_function	glutamine-fructose-6-phosphate transaminase (isomerizing) activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005975	biological_process	carbohydrate metabolic process
B	0006002	biological_process	fructose 6-phosphate metabolic process
B	0006047	biological_process	UDP-N-acetylglucosamine metabolic process
B	0006048	biological_process	UDP-N-acetylglucosamine biosynthetic process
B	0006487	biological_process	protein N-linked glycosylation
B	0008483	molecular_function	transaminase activity
B	0016740	molecular_function	transferase activity
B	0046349	biological_process	amino sugar biosynthetic process
B	0097367	molecular_function	carbohydrate derivative binding
B	1901135	biological_process	carbohydrate derivative metabolic process
B	1901137	biological_process	carbohydrate derivative biosynthetic process
C	0004360	molecular_function	glutamine-fructose-6-phosphate transaminase (isomerizing) activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005975	biological_process	carbohydrate metabolic process
C	0006002	biological_process	fructose 6-phosphate metabolic process
C	0006047	biological_process	UDP-N-acetylglucosamine metabolic process
C	0006048	biological_process	UDP-N-acetylglucosamine biosynthetic process
C	0006487	biological_process	protein N-linked glycosylation
C	0008483	molecular_function	transaminase activity
C	0016740	molecular_function	transferase activity
C	0046349	biological_process	amino sugar biosynthetic process
C	0097367	molecular_function	carbohydrate derivative binding
C	1901135	biological_process	carbohydrate derivative metabolic process
C	1901137	biological_process	carbohydrate derivative biosynthetic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	21
Details	BINDING SITE FOR RESIDUE F6R A 1609

Chain	Residue
A	CYS300
A	ALA400
A	SER401
A	LYS485
A	GLU488
A	HIS504
A	HOH2228
A	HOH2233
A	HOH2274
A	HOH2278
A	HOH2307
A	GLY301
A	HOH2308
A	HOH2427
A	THR302
A	SER303
A	SER347
A	GLN348
A	SER349
A	THR352
A	VAL399

site_id	AC2
Number of Residues	17
Details	BINDING SITE FOR RESIDUE F6R C 1609

Chain	Residue
B	HIS504
C	CYS300
C	GLY301
C	THR302
C	SER303
C	SER347
C	GLN348
C	SER349
C	THR352
C	ALA400
C	SER401
C	LYS485
C	GLU488
C	HOH2171
C	HOH2180
C	HOH2183
C	HOH2207

site_id	AC3
Number of Residues	16
Details	BINDING SITE FOR RESIDUE F6R B 1609

Chain	Residue
B	CYS300
B	GLY301
B	THR302
B	SER303
B	SER347
B	GLN348
B	SER349
B	THR352
B	VAL399
B	ALA400
B	SER401
B	LYS485
B	GLU488
B	HOH2014
B	HOH2018
C	HIS504

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	648
Details	Domain: {"description":"Glutamine amidotransferase type-2"}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	420
Details	Domain: {"description":"SIS 1"}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	423
Details	Domain: {"description":"SIS 2"}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	3
Details	Active site: {"description":"Nucleophile; for GATase activity","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	3
Details	Active site: {"description":"For Fru-6P isomerization activity"}

Chain

Residue

Details

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	10
Details	M-CSA 82

Chain	Residue	Details
A	CYS1	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
A	LYS603	covalently attached, electron pair acceptor, electron pair donor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
A	ARG26	activator, electrostatic stabiliser, hydrogen bond acceptor
A	TRP74	electrostatic stabiliser, hydrogen bond donor
A	ASN98	electrostatic stabiliser, hydrogen bond donor
A	GLY99	electrostatic stabiliser, hydrogen bond donor
A	GLU481	electrostatic stabiliser, hydrogen bond acceptor
A	LYS485	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	GLU488	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	HIS504	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

site_id	MCSA2
Number of Residues	10
Details	M-CSA 82

Chain	Residue	Details
B	CYS1	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
B	LYS603	covalently attached, electron pair acceptor, electron pair donor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
B	ARG26	activator, electrostatic stabiliser, hydrogen bond acceptor
B	TRP74	electrostatic stabiliser, hydrogen bond donor
B	ASN98	electrostatic stabiliser, hydrogen bond donor
B	GLY99	electrostatic stabiliser, hydrogen bond donor
B	GLU481	electrostatic stabiliser, hydrogen bond acceptor
B	LYS485	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
B	GLU488	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
B	HIS504	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

site_id	MCSA3
Number of Residues	10
Details	M-CSA 82

Chain	Residue	Details
C	CYS1	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
C	LYS603	covalently attached, electron pair acceptor, electron pair donor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
C	ARG26	activator, electrostatic stabiliser, hydrogen bond acceptor
C	TRP74	electrostatic stabiliser, hydrogen bond donor
C	ASN98	electrostatic stabiliser, hydrogen bond donor
C	GLY99	electrostatic stabiliser, hydrogen bond donor
C	GLU481	electrostatic stabiliser, hydrogen bond acceptor
C	LYS485	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
C	GLU488	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
C	HIS504	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)