4AJL

rat LDHA in complex with 3-(ethylcarbamoylamino)-N-(2-methyl-1,3- benzothiazol-6-yl)propanamide

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001666	biological_process	response to hypoxia
A	0001889	biological_process	liver development
A	0003824	molecular_function	catalytic activity
A	0004457	molecular_function	lactate dehydrogenase activity
A	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0006089	biological_process	lactate metabolic process
A	0007519	biological_process	skeletal muscle tissue development
A	0007584	biological_process	response to nutrient
A	0009410	biological_process	response to xenobiotic stimulus
A	0009749	biological_process	response to glucose
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0019661	biological_process	glucose catabolic process to lactate via pyruvate
A	0019674	biological_process	NAD+ metabolic process
A	0019752	biological_process	carboxylic acid metabolic process
A	0019900	molecular_function	kinase binding
A	0035686	cellular_component	sperm fibrous sheath
A	0042542	biological_process	response to hydrogen peroxide
A	0042802	molecular_function	identical protein binding
A	0042867	biological_process	pyruvate catabolic process
A	0043065	biological_process	positive regulation of apoptotic process
A	0043627	biological_process	response to estrogen
A	0051287	molecular_function	NAD binding
A	0051591	biological_process	response to cAMP
A	1904628	biological_process	cellular response to phorbol 13-acetate 12-myristate
A	1990204	cellular_component	oxidoreductase complex
B	0001666	biological_process	response to hypoxia
B	0001889	biological_process	liver development
B	0003824	molecular_function	catalytic activity
B	0004457	molecular_function	lactate dehydrogenase activity
B	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005829	cellular_component	cytosol
B	0006089	biological_process	lactate metabolic process
B	0007519	biological_process	skeletal muscle tissue development
B	0007584	biological_process	response to nutrient
B	0009410	biological_process	response to xenobiotic stimulus
B	0009749	biological_process	response to glucose
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0019661	biological_process	glucose catabolic process to lactate via pyruvate
B	0019674	biological_process	NAD+ metabolic process
B	0019752	biological_process	carboxylic acid metabolic process
B	0019900	molecular_function	kinase binding
B	0035686	cellular_component	sperm fibrous sheath
B	0042542	biological_process	response to hydrogen peroxide
B	0042802	molecular_function	identical protein binding
B	0042867	biological_process	pyruvate catabolic process
B	0043065	biological_process	positive regulation of apoptotic process
B	0043627	biological_process	response to estrogen
B	0051287	molecular_function	NAD binding
B	0051591	biological_process	response to cAMP
B	1904628	biological_process	cellular response to phorbol 13-acetate 12-myristate
B	1990204	cellular_component	oxidoreductase complex
C	0001666	biological_process	response to hypoxia
C	0001889	biological_process	liver development
C	0003824	molecular_function	catalytic activity
C	0004457	molecular_function	lactate dehydrogenase activity
C	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005829	cellular_component	cytosol
C	0006089	biological_process	lactate metabolic process
C	0007519	biological_process	skeletal muscle tissue development
C	0007584	biological_process	response to nutrient
C	0009410	biological_process	response to xenobiotic stimulus
C	0009749	biological_process	response to glucose
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0019661	biological_process	glucose catabolic process to lactate via pyruvate
C	0019674	biological_process	NAD+ metabolic process
C	0019752	biological_process	carboxylic acid metabolic process
C	0019900	molecular_function	kinase binding
C	0035686	cellular_component	sperm fibrous sheath
C	0042542	biological_process	response to hydrogen peroxide
C	0042802	molecular_function	identical protein binding
C	0042867	biological_process	pyruvate catabolic process
C	0043065	biological_process	positive regulation of apoptotic process
C	0043627	biological_process	response to estrogen
C	0051287	molecular_function	NAD binding
C	0051591	biological_process	response to cAMP
C	1904628	biological_process	cellular response to phorbol 13-acetate 12-myristate
C	1990204	cellular_component	oxidoreductase complex
D	0001666	biological_process	response to hypoxia
D	0001889	biological_process	liver development
D	0003824	molecular_function	catalytic activity
D	0004457	molecular_function	lactate dehydrogenase activity
D	0004459	molecular_function	L-lactate dehydrogenase (NAD+) activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005829	cellular_component	cytosol
D	0006089	biological_process	lactate metabolic process
D	0007519	biological_process	skeletal muscle tissue development
D	0007584	biological_process	response to nutrient
D	0009410	biological_process	response to xenobiotic stimulus
D	0009749	biological_process	response to glucose
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0019661	biological_process	glucose catabolic process to lactate via pyruvate
D	0019674	biological_process	NAD+ metabolic process
D	0019752	biological_process	carboxylic acid metabolic process
D	0019900	molecular_function	kinase binding
D	0035686	cellular_component	sperm fibrous sheath
D	0042542	biological_process	response to hydrogen peroxide
D	0042802	molecular_function	identical protein binding
D	0042867	biological_process	pyruvate catabolic process
D	0043065	biological_process	positive regulation of apoptotic process
D	0043627	biological_process	response to estrogen
D	0051287	molecular_function	NAD binding
D	0051591	biological_process	response to cAMP
D	1904628	biological_process	cellular response to phorbol 13-acetate 12-myristate
D	1990204	cellular_component	oxidoreductase complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GOL C 1332

Chain	Residue
C	ASN163
C	HOH2236
C	HOH2246
D	LEU69
C	SER166
C	ALA167
C	ARG170
C	VAL269
C	HIS270
C	PRO271
C	MLI1338
C	HOH2189

---

site_id	AC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL C 1334

Chain	Residue
B	SER201
B	GLY202
B	ASN204
B	GLY207
C	SER201
C	GLY202
C	GLY207
C	SER209

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL B 1332

Chain	Residue
A	MET40
A	HOH2048
B	MET40
B	ASP42
B	PHE70
B	LEU71
B	HOH2092

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL D 1332

Chain	Residue
C	LYS41
D	MET40
D	ASP42
D	HOH2034
D	HOH2061

---

site_id	AC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 1332

Chain	Residue
A	SER201
A	GLY202
A	ASN204
A	GLY207
A	SER209
D	GLY202
D	ASN204
D	GLY207

---

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL C 1335

Chain	Residue
C	SER104
C	ASN107
C	HOH2274
C	HOH2276

---

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE DMS C 1333

Chain	Residue
C	GLY96
C	ALA97
C	88W1336
C	HOH2272
C	HOH2273

---

site_id	AC8
Number of Residues	12
Details	BINDING SITE FOR RESIDUE 88W A 1333

Chain	Residue
A	GLY26
A	GLY28
A	ASP51
A	VAL52
A	THR94
A	ALA95
A	GLY96
A	PHE118
A	ILE119
A	HOH2031
A	HOH2035
A	HOH2124

---

site_id	AC9
Number of Residues	10
Details	BINDING SITE FOR RESIDUE 88W B 1333

Chain	Residue
B	ASP51
B	VAL52
B	THR94
B	ALA95
B	GLY96
B	ARG98
B	ILE119
B	HOH2032
B	HOH2036
B	HOH2106

---

site_id	BC1
Number of Residues	13
Details	BINDING SITE FOR RESIDUE 88W C 1336

Chain	Residue
C	GLY28
C	ASP51
C	VAL52
C	THR94
C	ALA95
C	GLY96
C	PHE118
C	ILE119
C	DMS1333
C	HOH2018
C	HOH2022
C	HOH2045
C	HOH2093

---

site_id	BC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE 88W D 1333

Chain	Residue
D	GLY28
D	ASP51
D	VAL52
D	THR94
D	ALA95
D	GLY96
D	ILE119
D	HOH2020
D	HOH2024
D	HOH2076

---

site_id	BC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE MLI C 1337

Chain	Residue
C	HIS192
C	ALA237
C	THR247
C	GLN99
C	ARG105
C	ASN137
C	LEU164
C	ARG168

---

site_id	BC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE MLI A 1334

Chain	Residue
A	ARG105
A	ASN137
A	LEU164
A	ARG168
A	HIS192
A	ALA237
A	THR247
A	HOH2201
A	HOH2302

---

site_id	BC5
Number of Residues	11
Details	BINDING SITE FOR RESIDUE MLI B 1334

Chain	Residue
B	ARG170
B	HIS185
B	TRP187
B	VAL269
B	HOH2195
B	HOH2210
B	HOH2281
B	HOH2325
B	HOH2326
C	SER183
C	HIS185

---

site_id	BC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MLI B 1335

Chain	Residue
B	GLN99
B	ARG105
B	ASN137
B	ARG168
B	HIS192
B	ALA237
B	THR247

---

site_id	BC7
Number of Residues	10
Details	BINDING SITE FOR RESIDUE MLI A 1335

Chain	Residue
A	ARG170
A	HIS185
A	VAL269
A	HOH2230
A	HOH2254
A	HOH2327
A	HOH2356
A	HOH2357
D	SER183
D	HIS185

---

site_id	BC8
Number of Residues	12
Details	BINDING SITE FOR RESIDUE MLI C 1338

Chain	Residue
B	SER183
B	HIS185
B	HOH2205
B	HOH2206
B	HOH2207
C	ARG170
C	HIS185
C	VAL269
C	GOL1332
C	HOH2177
C	HOH2243
C	HOH2246

---

site_id	BC9
Number of Residues	10
Details	BINDING SITE FOR RESIDUE MLI D 1334

Chain	Residue
D	ARG105
D	ASN137
D	LEU164
D	ARG168
D	HIS192
D	ALA237
D	THR247
D	HOH2092
D	HOH2120
D	HOH2185

---

site_id	CC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE MLI D 1335

Chain	Residue
A	SER183
A	HIS185
A	HOH2249
A	HOH2250
A	HOH2251
D	ARG170
D	HIS185
D	VAL269
D	HOH2144
D	HOH2199

---

Functional Information from PROSITE/UniProt

site_id	PS00064
Number of Residues	7
Details	L_LDH L-lactate dehydrogenase active site. LGEHGDS

Chain	Residue	Details
A	LEU189-SER195

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	132
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	12
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P06151","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P00338","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P00338","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"22673903","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	12
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P06151","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"22673903","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	8
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"22673903","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	8
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate","evidences":[{"source":"UniProtKB","id":"P00338","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	Modified residue: {"description":"N-acetylalanine","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JUN-2009","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","von Kriegsheim A.F.","Kolch W."]}}]}

Chain

Residue

Details

---