4AJL
rat LDHA in complex with 3-(ethylcarbamoylamino)-N-(2-methyl-1,3- benzothiazol-6-yl)propanamide
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0001666 | biological_process | response to hypoxia |
| A | 0001889 | biological_process | liver development |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004457 | molecular_function | lactate dehydrogenase activity |
| A | 0004459 | molecular_function | L-lactate dehydrogenase (NAD+) activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005829 | cellular_component | cytosol |
| A | 0006089 | biological_process | lactate metabolic process |
| A | 0007519 | biological_process | skeletal muscle tissue development |
| A | 0007584 | biological_process | response to nutrient |
| A | 0009410 | biological_process | response to xenobiotic stimulus |
| A | 0009749 | biological_process | response to glucose |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0019661 | biological_process | glucose catabolic process to lactate via pyruvate |
| A | 0019674 | biological_process | NAD+ metabolic process |
| A | 0019752 | biological_process | carboxylic acid metabolic process |
| A | 0019900 | molecular_function | kinase binding |
| A | 0035686 | cellular_component | sperm fibrous sheath |
| A | 0042542 | biological_process | response to hydrogen peroxide |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0042867 | biological_process | pyruvate catabolic process |
| A | 0043065 | biological_process | positive regulation of apoptotic process |
| A | 0043627 | biological_process | response to estrogen |
| A | 0051287 | molecular_function | NAD binding |
| A | 0051591 | biological_process | response to cAMP |
| A | 1904628 | biological_process | cellular response to phorbol 13-acetate 12-myristate |
| A | 1990204 | cellular_component | oxidoreductase complex |
| B | 0001666 | biological_process | response to hypoxia |
| B | 0001889 | biological_process | liver development |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004457 | molecular_function | lactate dehydrogenase activity |
| B | 0004459 | molecular_function | L-lactate dehydrogenase (NAD+) activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005829 | cellular_component | cytosol |
| B | 0006089 | biological_process | lactate metabolic process |
| B | 0007519 | biological_process | skeletal muscle tissue development |
| B | 0007584 | biological_process | response to nutrient |
| B | 0009410 | biological_process | response to xenobiotic stimulus |
| B | 0009749 | biological_process | response to glucose |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0019661 | biological_process | glucose catabolic process to lactate via pyruvate |
| B | 0019674 | biological_process | NAD+ metabolic process |
| B | 0019752 | biological_process | carboxylic acid metabolic process |
| B | 0019900 | molecular_function | kinase binding |
| B | 0035686 | cellular_component | sperm fibrous sheath |
| B | 0042542 | biological_process | response to hydrogen peroxide |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0042867 | biological_process | pyruvate catabolic process |
| B | 0043065 | biological_process | positive regulation of apoptotic process |
| B | 0043627 | biological_process | response to estrogen |
| B | 0051287 | molecular_function | NAD binding |
| B | 0051591 | biological_process | response to cAMP |
| B | 1904628 | biological_process | cellular response to phorbol 13-acetate 12-myristate |
| B | 1990204 | cellular_component | oxidoreductase complex |
| C | 0001666 | biological_process | response to hypoxia |
| C | 0001889 | biological_process | liver development |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004457 | molecular_function | lactate dehydrogenase activity |
| C | 0004459 | molecular_function | L-lactate dehydrogenase (NAD+) activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005739 | cellular_component | mitochondrion |
| C | 0005829 | cellular_component | cytosol |
| C | 0006089 | biological_process | lactate metabolic process |
| C | 0007519 | biological_process | skeletal muscle tissue development |
| C | 0007584 | biological_process | response to nutrient |
| C | 0009410 | biological_process | response to xenobiotic stimulus |
| C | 0009749 | biological_process | response to glucose |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| C | 0019661 | biological_process | glucose catabolic process to lactate via pyruvate |
| C | 0019674 | biological_process | NAD+ metabolic process |
| C | 0019752 | biological_process | carboxylic acid metabolic process |
| C | 0019900 | molecular_function | kinase binding |
| C | 0035686 | cellular_component | sperm fibrous sheath |
| C | 0042542 | biological_process | response to hydrogen peroxide |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0042867 | biological_process | pyruvate catabolic process |
| C | 0043065 | biological_process | positive regulation of apoptotic process |
| C | 0043627 | biological_process | response to estrogen |
| C | 0051287 | molecular_function | NAD binding |
| C | 0051591 | biological_process | response to cAMP |
| C | 1904628 | biological_process | cellular response to phorbol 13-acetate 12-myristate |
| C | 1990204 | cellular_component | oxidoreductase complex |
| D | 0001666 | biological_process | response to hypoxia |
| D | 0001889 | biological_process | liver development |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004457 | molecular_function | lactate dehydrogenase activity |
| D | 0004459 | molecular_function | L-lactate dehydrogenase (NAD+) activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005739 | cellular_component | mitochondrion |
| D | 0005829 | cellular_component | cytosol |
| D | 0006089 | biological_process | lactate metabolic process |
| D | 0007519 | biological_process | skeletal muscle tissue development |
| D | 0007584 | biological_process | response to nutrient |
| D | 0009410 | biological_process | response to xenobiotic stimulus |
| D | 0009749 | biological_process | response to glucose |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| D | 0019661 | biological_process | glucose catabolic process to lactate via pyruvate |
| D | 0019674 | biological_process | NAD+ metabolic process |
| D | 0019752 | biological_process | carboxylic acid metabolic process |
| D | 0019900 | molecular_function | kinase binding |
| D | 0035686 | cellular_component | sperm fibrous sheath |
| D | 0042542 | biological_process | response to hydrogen peroxide |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0042867 | biological_process | pyruvate catabolic process |
| D | 0043065 | biological_process | positive regulation of apoptotic process |
| D | 0043627 | biological_process | response to estrogen |
| D | 0051287 | molecular_function | NAD binding |
| D | 0051591 | biological_process | response to cAMP |
| D | 1904628 | biological_process | cellular response to phorbol 13-acetate 12-myristate |
| D | 1990204 | cellular_component | oxidoreductase complex |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE GOL C 1332 |
| Chain | Residue |
| C | ASN163 |
| C | HOH2236 |
| C | HOH2246 |
| D | LEU69 |
| C | SER166 |
| C | ALA167 |
| C | ARG170 |
| C | VAL269 |
| C | HIS270 |
| C | PRO271 |
| C | MLI1338 |
| C | HOH2189 |
| site_id | AC2 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL C 1334 |
| Chain | Residue |
| B | SER201 |
| B | GLY202 |
| B | ASN204 |
| B | GLY207 |
| C | SER201 |
| C | GLY202 |
| C | GLY207 |
| C | SER209 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL B 1332 |
| Chain | Residue |
| A | MET40 |
| A | HOH2048 |
| B | MET40 |
| B | ASP42 |
| B | PHE70 |
| B | LEU71 |
| B | HOH2092 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL D 1332 |
| Chain | Residue |
| C | LYS41 |
| D | MET40 |
| D | ASP42 |
| D | HOH2034 |
| D | HOH2061 |
| site_id | AC5 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL A 1332 |
| Chain | Residue |
| A | SER201 |
| A | GLY202 |
| A | ASN204 |
| A | GLY207 |
| A | SER209 |
| D | GLY202 |
| D | ASN204 |
| D | GLY207 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL C 1335 |
| Chain | Residue |
| C | SER104 |
| C | ASN107 |
| C | HOH2274 |
| C | HOH2276 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS C 1333 |
| Chain | Residue |
| C | GLY96 |
| C | ALA97 |
| C | 88W1336 |
| C | HOH2272 |
| C | HOH2273 |
| site_id | AC8 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE 88W A 1333 |
| Chain | Residue |
| A | GLY26 |
| A | GLY28 |
| A | ASP51 |
| A | VAL52 |
| A | THR94 |
| A | ALA95 |
| A | GLY96 |
| A | PHE118 |
| A | ILE119 |
| A | HOH2031 |
| A | HOH2035 |
| A | HOH2124 |
| site_id | AC9 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE 88W B 1333 |
| Chain | Residue |
| B | ASP51 |
| B | VAL52 |
| B | THR94 |
| B | ALA95 |
| B | GLY96 |
| B | ARG98 |
| B | ILE119 |
| B | HOH2032 |
| B | HOH2036 |
| B | HOH2106 |
| site_id | BC1 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE 88W C 1336 |
| Chain | Residue |
| C | GLY28 |
| C | ASP51 |
| C | VAL52 |
| C | THR94 |
| C | ALA95 |
| C | GLY96 |
| C | PHE118 |
| C | ILE119 |
| C | DMS1333 |
| C | HOH2018 |
| C | HOH2022 |
| C | HOH2045 |
| C | HOH2093 |
| site_id | BC2 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE 88W D 1333 |
| Chain | Residue |
| D | GLY28 |
| D | ASP51 |
| D | VAL52 |
| D | THR94 |
| D | ALA95 |
| D | GLY96 |
| D | ILE119 |
| D | HOH2020 |
| D | HOH2024 |
| D | HOH2076 |
| site_id | BC3 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE MLI C 1337 |
| Chain | Residue |
| C | HIS192 |
| C | ALA237 |
| C | THR247 |
| C | GLN99 |
| C | ARG105 |
| C | ASN137 |
| C | LEU164 |
| C | ARG168 |
| site_id | BC4 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE MLI A 1334 |
| Chain | Residue |
| A | ARG105 |
| A | ASN137 |
| A | LEU164 |
| A | ARG168 |
| A | HIS192 |
| A | ALA237 |
| A | THR247 |
| A | HOH2201 |
| A | HOH2302 |
| site_id | BC5 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE MLI B 1334 |
| Chain | Residue |
| B | ARG170 |
| B | HIS185 |
| B | TRP187 |
| B | VAL269 |
| B | HOH2195 |
| B | HOH2210 |
| B | HOH2281 |
| B | HOH2325 |
| B | HOH2326 |
| C | SER183 |
| C | HIS185 |
| site_id | BC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE MLI B 1335 |
| Chain | Residue |
| B | GLN99 |
| B | ARG105 |
| B | ASN137 |
| B | ARG168 |
| B | HIS192 |
| B | ALA237 |
| B | THR247 |
| site_id | BC7 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE MLI A 1335 |
| Chain | Residue |
| A | ARG170 |
| A | HIS185 |
| A | VAL269 |
| A | HOH2230 |
| A | HOH2254 |
| A | HOH2327 |
| A | HOH2356 |
| A | HOH2357 |
| D | SER183 |
| D | HIS185 |
| site_id | BC8 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE MLI C 1338 |
| Chain | Residue |
| B | SER183 |
| B | HIS185 |
| B | HOH2205 |
| B | HOH2206 |
| B | HOH2207 |
| C | ARG170 |
| C | HIS185 |
| C | VAL269 |
| C | GOL1332 |
| C | HOH2177 |
| C | HOH2243 |
| C | HOH2246 |
| site_id | BC9 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE MLI D 1334 |
| Chain | Residue |
| D | ARG105 |
| D | ASN137 |
| D | LEU164 |
| D | ARG168 |
| D | HIS192 |
| D | ALA237 |
| D | THR247 |
| D | HOH2092 |
| D | HOH2120 |
| D | HOH2185 |
| site_id | CC1 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE MLI D 1335 |
| Chain | Residue |
| A | SER183 |
| A | HIS185 |
| A | HOH2249 |
| A | HOH2250 |
| A | HOH2251 |
| D | ARG170 |
| D | HIS185 |
| D | VAL269 |
| D | HOH2144 |
| D | HOH2199 |
Functional Information from PROSITE/UniProt
| site_id | PS00064 |
| Number of Residues | 7 |
| Details | L_LDH L-lactate dehydrogenase active site. LGEHGDS |
| Chain | Residue | Details |
| A | LEU189-SER195 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton acceptor => ECO:0000250 |
| Chain | Residue | Details |
| A | HIS192 | |
| B | HIS192 | |
| C | HIS192 | |
| D | HIS192 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 24 |
| Details | BINDING: BINDING => ECO:0000250 |
| Chain | Residue | Details |
| A | GLY28 | |
| B | ASN137 | |
| B | ARG168 | |
| B | THR247 | |
| C | GLY28 | |
| C | ARG98 | |
| C | ARG105 | |
| C | ASN137 | |
| C | ARG168 | |
| C | THR247 | |
| D | GLY28 | |
| A | ARG98 | |
| D | ARG98 | |
| D | ARG105 | |
| D | ASN137 | |
| D | ARG168 | |
| D | THR247 | |
| A | ARG105 | |
| A | ASN137 | |
| A | ARG168 | |
| A | THR247 | |
| B | GLY28 | |
| B | ARG98 | |
| B | ARG105 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | MOD_RES: N-acetylalanine => ECO:0000269|Ref.3 |
| Chain | Residue | Details |
| A | ALA1 | |
| B | ALA1 | |
| C | ALA1 | |
| D | ALA1 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 12 |
| Details | MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P06151 |
| Chain | Residue | Details |
| A | LYS4 | |
| D | LYS4 | |
| D | LYS117 | |
| D | LYS317 | |
| A | LYS117 | |
| A | LYS317 | |
| B | LYS4 | |
| B | LYS117 | |
| B | LYS317 | |
| C | LYS4 | |
| C | LYS117 | |
| C | LYS317 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 12 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P00338 |
| Chain | Residue | Details |
| A | LYS13 | |
| D | LYS13 | |
| D | LYS80 | |
| D | LYS125 | |
| A | LYS80 | |
| A | LYS125 | |
| B | LYS13 | |
| B | LYS80 | |
| B | LYS125 | |
| C | LYS13 | |
| C | LYS80 | |
| C | LYS125 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | MOD_RES: N6-acetyllysine; alternate => ECO:0000250|UniProtKB:P00338 |
| Chain | Residue | Details |
| A | LYS56 | |
| B | LYS56 | |
| C | LYS56 | |
| D | LYS56 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:22673903 |
| Chain | Residue | Details |
| A | SER212 | |
| B | SER212 | |
| C | SER212 | |
| D | SER212 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 12 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P06151 |
| Chain | Residue | Details |
| A | LYS223 | |
| D | LYS223 | |
| D | LYS231 | |
| D | LYS242 | |
| A | LYS231 | |
| A | LYS242 | |
| B | LYS223 | |
| B | LYS231 | |
| B | LYS242 | |
| C | LYS223 | |
| C | LYS231 | |
| C | LYS242 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:22673903 |
| Chain | Residue | Details |
| A | TYR238 | |
| B | TYR238 | |
| C | TYR238 | |
| D | TYR238 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 8 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:22673903 |
| Chain | Residue | Details |
| A | THR308 | |
| A | THR321 | |
| B | THR308 | |
| B | THR321 | |
| C | THR308 | |
| C | THR321 | |
| D | THR308 | |
| D | THR321 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 8 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0000250|UniProtKB:P00338 |
| Chain | Residue | Details |
| A | LYS56 | |
| B | LYS56 | |
| C | LYS56 | |
| D | LYS56 |
