Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4A8A

Asymmetric cryo-EM reconstruction of E. coli DegQ 12-mer in complex with lysozyme

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004175	molecular_function	endopeptidase activity
A	0004252	molecular_function	serine-type endopeptidase activity
A	0005515	molecular_function	protein binding
A	0006508	biological_process	proteolysis
A	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
A	0008233	molecular_function	peptidase activity
A	0008236	molecular_function	serine-type peptidase activity
A	0012501	biological_process	programmed cell death
A	0016787	molecular_function	hydrolase activity
A	0042597	cellular_component	periplasmic space
A	0042802	molecular_function	identical protein binding
A	0043065	biological_process	positive regulation of apoptotic process
A	0051603	biological_process	proteolysis involved in protein catabolic process
B	0004175	molecular_function	endopeptidase activity
B	0004252	molecular_function	serine-type endopeptidase activity
B	0005515	molecular_function	protein binding
B	0006508	biological_process	proteolysis
B	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
B	0008233	molecular_function	peptidase activity
B	0008236	molecular_function	serine-type peptidase activity
B	0012501	biological_process	programmed cell death
B	0016787	molecular_function	hydrolase activity
B	0042597	cellular_component	periplasmic space
B	0042802	molecular_function	identical protein binding
B	0043065	biological_process	positive regulation of apoptotic process
B	0051603	biological_process	proteolysis involved in protein catabolic process
C	0004175	molecular_function	endopeptidase activity
C	0004252	molecular_function	serine-type endopeptidase activity
C	0005515	molecular_function	protein binding
C	0006508	biological_process	proteolysis
C	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
C	0008233	molecular_function	peptidase activity
C	0008236	molecular_function	serine-type peptidase activity
C	0012501	biological_process	programmed cell death
C	0016787	molecular_function	hydrolase activity
C	0042597	cellular_component	periplasmic space
C	0042802	molecular_function	identical protein binding
C	0043065	biological_process	positive regulation of apoptotic process
C	0051603	biological_process	proteolysis involved in protein catabolic process
D	0004175	molecular_function	endopeptidase activity
D	0004252	molecular_function	serine-type endopeptidase activity
D	0005515	molecular_function	protein binding
D	0006508	biological_process	proteolysis
D	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
D	0008233	molecular_function	peptidase activity
D	0008236	molecular_function	serine-type peptidase activity
D	0012501	biological_process	programmed cell death
D	0016787	molecular_function	hydrolase activity
D	0042597	cellular_component	periplasmic space
D	0042802	molecular_function	identical protein binding
D	0043065	biological_process	positive regulation of apoptotic process
D	0051603	biological_process	proteolysis involved in protein catabolic process
E	0004175	molecular_function	endopeptidase activity
E	0004252	molecular_function	serine-type endopeptidase activity
E	0005515	molecular_function	protein binding
E	0006508	biological_process	proteolysis
E	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
E	0008233	molecular_function	peptidase activity
E	0008236	molecular_function	serine-type peptidase activity
E	0012501	biological_process	programmed cell death
E	0016787	molecular_function	hydrolase activity
E	0042597	cellular_component	periplasmic space
E	0042802	molecular_function	identical protein binding
E	0043065	biological_process	positive regulation of apoptotic process
E	0051603	biological_process	proteolysis involved in protein catabolic process
F	0004175	molecular_function	endopeptidase activity
F	0004252	molecular_function	serine-type endopeptidase activity
F	0005515	molecular_function	protein binding
F	0006508	biological_process	proteolysis
F	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
F	0008233	molecular_function	peptidase activity
F	0008236	molecular_function	serine-type peptidase activity
F	0012501	biological_process	programmed cell death
F	0016787	molecular_function	hydrolase activity
F	0042597	cellular_component	periplasmic space
F	0042802	molecular_function	identical protein binding
F	0043065	biological_process	positive regulation of apoptotic process
F	0051603	biological_process	proteolysis involved in protein catabolic process
G	0004175	molecular_function	endopeptidase activity
G	0004252	molecular_function	serine-type endopeptidase activity
G	0005515	molecular_function	protein binding
G	0006508	biological_process	proteolysis
G	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
G	0008233	molecular_function	peptidase activity
G	0008236	molecular_function	serine-type peptidase activity
G	0012501	biological_process	programmed cell death
G	0016787	molecular_function	hydrolase activity
G	0042597	cellular_component	periplasmic space
G	0042802	molecular_function	identical protein binding
G	0043065	biological_process	positive regulation of apoptotic process
G	0051603	biological_process	proteolysis involved in protein catabolic process
H	0004175	molecular_function	endopeptidase activity
H	0004252	molecular_function	serine-type endopeptidase activity
H	0005515	molecular_function	protein binding
H	0006508	biological_process	proteolysis
H	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
H	0008233	molecular_function	peptidase activity
H	0008236	molecular_function	serine-type peptidase activity
H	0012501	biological_process	programmed cell death
H	0016787	molecular_function	hydrolase activity
H	0042597	cellular_component	periplasmic space
H	0042802	molecular_function	identical protein binding
H	0043065	biological_process	positive regulation of apoptotic process
H	0051603	biological_process	proteolysis involved in protein catabolic process
I	0004175	molecular_function	endopeptidase activity
I	0004252	molecular_function	serine-type endopeptidase activity
I	0005515	molecular_function	protein binding
I	0006508	biological_process	proteolysis
I	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
I	0008233	molecular_function	peptidase activity
I	0008236	molecular_function	serine-type peptidase activity
I	0012501	biological_process	programmed cell death
I	0016787	molecular_function	hydrolase activity
I	0042597	cellular_component	periplasmic space
I	0042802	molecular_function	identical protein binding
I	0043065	biological_process	positive regulation of apoptotic process
I	0051603	biological_process	proteolysis involved in protein catabolic process
J	0004175	molecular_function	endopeptidase activity
J	0004252	molecular_function	serine-type endopeptidase activity
J	0005515	molecular_function	protein binding
J	0006508	biological_process	proteolysis
J	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
J	0008233	molecular_function	peptidase activity
J	0008236	molecular_function	serine-type peptidase activity
J	0012501	biological_process	programmed cell death
J	0016787	molecular_function	hydrolase activity
J	0042597	cellular_component	periplasmic space
J	0042802	molecular_function	identical protein binding
J	0043065	biological_process	positive regulation of apoptotic process
J	0051603	biological_process	proteolysis involved in protein catabolic process
K	0004175	molecular_function	endopeptidase activity
K	0004252	molecular_function	serine-type endopeptidase activity
K	0005515	molecular_function	protein binding
K	0006508	biological_process	proteolysis
K	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
K	0008233	molecular_function	peptidase activity
K	0008236	molecular_function	serine-type peptidase activity
K	0012501	biological_process	programmed cell death
K	0016787	molecular_function	hydrolase activity
K	0042597	cellular_component	periplasmic space
K	0042802	molecular_function	identical protein binding
K	0043065	biological_process	positive regulation of apoptotic process
K	0051603	biological_process	proteolysis involved in protein catabolic process
L	0004175	molecular_function	endopeptidase activity
L	0004252	molecular_function	serine-type endopeptidase activity
L	0005515	molecular_function	protein binding
L	0006508	biological_process	proteolysis
L	0006515	biological_process	protein quality control for misfolded or incompletely synthesized proteins
L	0008233	molecular_function	peptidase activity
L	0008236	molecular_function	serine-type peptidase activity
L	0012501	biological_process	programmed cell death
L	0016787	molecular_function	hydrolase activity
L	0042597	cellular_component	periplasmic space
L	0042802	molecular_function	identical protein binding
L	0043065	biological_process	positive regulation of apoptotic process
L	0051603	biological_process	proteolysis involved in protein catabolic process
M	0003796	molecular_function	lysozyme activity
M	0003824	molecular_function	catalytic activity
M	0005515	molecular_function	protein binding
M	0005576	cellular_component	extracellular region
M	0005615	cellular_component	extracellular space
M	0005737	cellular_component	cytoplasm
M	0005783	cellular_component	endoplasmic reticulum
M	0008152	biological_process	metabolic process
M	0016231	molecular_function	beta-N-acetylglucosaminidase activity
M	0016787	molecular_function	hydrolase activity
M	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
M	0016998	biological_process	cell wall macromolecule catabolic process
M	0031640	biological_process	killing of cells of another organism
M	0042742	biological_process	defense response to bacterium
M	0042802	molecular_function	identical protein binding
M	0050829	biological_process	defense response to Gram-negative bacterium
M	0050830	biological_process	defense response to Gram-positive bacterium
M	0051672	biological_process	obsolete catabolism by organism of cell wall peptidoglycan in other organism

Functional Information from PROSITE/UniProt

site_id	PS00128
Number of Residues	19
Details	GLYCOSYL_HYDROL_F22_1 Glycosyl hydrolases family 22 (GH22) domain signature. CnipCsaLlssDItasvnC

Chain	Residue	Details
M	CYS76-CYS94

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE:

Chain	Residue	Details
M	GLU35
D	HIS82
D	ASP112
D	ALA187
E	HIS82
E	ASP112
E	ALA187
F	HIS82
F	ASP112
F	ALA187
G	HIS82
M	ASP52
G	ASP112
G	ALA187
H	HIS82
H	ASP112
H	ALA187
I	HIS82
I	ASP112
I	ALA187
J	HIS82
J	ASP112
A	ALA187
J	ALA187
K	HIS82
K	ASP112
K	ALA187
L	HIS82
L	ASP112
L	ALA187
B	HIS82
B	ASP112
B	ALA187
C	HIS82
C	ASP112
C	ALA187

site_id	SWS_FT_FI2
Number of Residues	1
Details	BINDING:

Chain	Residue	Details
M	ASP101
D	GLU32
D	HIS82
D	ASP112
E	GLU32
E	HIS82
E	ASP112
F	GLU32
F	HIS82
F	ASP112
G	GLU32
A	HIS82
G	HIS82
G	ASP112
H	GLU32
H	HIS82
H	ASP112
I	GLU32
I	HIS82
I	ASP112
J	GLU32
J	HIS82
A	ASP112
J	ASP112
K	GLU32
K	HIS82
K	ASP112
L	GLU32
L	HIS82
L	ASP112
B	GLU32
B	HIS82
B	ASP112
C	GLU32
C	HIS82
C	ASP112

site_id	SWS_FT_FI3
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:21685389

Chain	Residue	Details
A	GLY185
J	GLY185
K	GLY185
L	GLY185
B	GLY185
C	GLY185
D	GLY185
E	GLY185
F	GLY185
G	GLY185
H	GLY185
I	GLY185

site_id	SWS_FT_FI4
Number of Residues	24
Details	BINDING:

Chain	Residue	Details
A	THR203
E	LEU242
F	THR203
F	LEU242
G	THR203
G	LEU242
H	THR203
H	LEU242
I	THR203
I	LEU242
J	THR203
A	LEU242
J	LEU242
K	THR203
K	LEU242
L	THR203
L	LEU242
B	THR203
B	LEU242
C	THR203
C	LEU242
D	THR203
D	LEU242
E	THR203

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 203

Chain	Residue	Details
M	GLU35	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
M	ASN46
M	ASP48
M	SER50
M	ASP52	covalently attached, electrostatic stabiliser, nucleofuge, nucleophile, polar/non-polar interaction
M	ASN59

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)