3ZWJ

CRYSTAL STRUCTURE OF THE PORE-FORMING TOXIN FRAC FROM ACTINIA FRAGACEA (Form 3)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005576	cellular_component	extracellular region
A	0006811	biological_process	monoatomic ion transport
A	0006812	biological_process	monoatomic cation transport
A	0008289	molecular_function	lipid binding
A	0015267	molecular_function	channel activity
A	0016020	cellular_component	membrane
A	0031640	biological_process	killing of cells of another organism
A	0035821	biological_process	modulation of process of another organism
A	0042151	cellular_component	nematocyst
A	0042802	molecular_function	identical protein binding
A	0044218	cellular_component	other organism cell membrane
A	0046930	cellular_component	pore complex
A	0046931	biological_process	pore complex assembly
A	0051715	biological_process	cytolysis in another organism
A	0055085	biological_process	transmembrane transport
A	0090729	molecular_function	toxin activity
B	0005576	cellular_component	extracellular region
B	0006811	biological_process	monoatomic ion transport
B	0006812	biological_process	monoatomic cation transport
B	0008289	molecular_function	lipid binding
B	0015267	molecular_function	channel activity
B	0016020	cellular_component	membrane
B	0031640	biological_process	killing of cells of another organism
B	0035821	biological_process	modulation of process of another organism
B	0042151	cellular_component	nematocyst
B	0042802	molecular_function	identical protein binding
B	0044218	cellular_component	other organism cell membrane
B	0046930	cellular_component	pore complex
B	0046931	biological_process	pore complex assembly
B	0051715	biological_process	cytolysis in another organism
B	0055085	biological_process	transmembrane transport
B	0090729	molecular_function	toxin activity
C	0005576	cellular_component	extracellular region
C	0006811	biological_process	monoatomic ion transport
C	0006812	biological_process	monoatomic cation transport
C	0008289	molecular_function	lipid binding
C	0015267	molecular_function	channel activity
C	0016020	cellular_component	membrane
C	0031640	biological_process	killing of cells of another organism
C	0035821	biological_process	modulation of process of another organism
C	0042151	cellular_component	nematocyst
C	0042802	molecular_function	identical protein binding
C	0044218	cellular_component	other organism cell membrane
C	0046930	cellular_component	pore complex
C	0046931	biological_process	pore complex assembly
C	0051715	biological_process	cytolysis in another organism
C	0055085	biological_process	transmembrane transport
C	0090729	molecular_function	toxin activity
D	0005576	cellular_component	extracellular region
D	0006811	biological_process	monoatomic ion transport
D	0006812	biological_process	monoatomic cation transport
D	0008289	molecular_function	lipid binding
D	0015267	molecular_function	channel activity
D	0016020	cellular_component	membrane
D	0031640	biological_process	killing of cells of another organism
D	0035821	biological_process	modulation of process of another organism
D	0042151	cellular_component	nematocyst
D	0042802	molecular_function	identical protein binding
D	0044218	cellular_component	other organism cell membrane
D	0046930	cellular_component	pore complex
D	0046931	biological_process	pore complex assembly
D	0051715	biological_process	cytolysis in another organism
D	0055085	biological_process	transmembrane transport
D	0090729	molecular_function	toxin activity
E	0005576	cellular_component	extracellular region
E	0006811	biological_process	monoatomic ion transport
E	0006812	biological_process	monoatomic cation transport
E	0008289	molecular_function	lipid binding
E	0015267	molecular_function	channel activity
E	0016020	cellular_component	membrane
E	0031640	biological_process	killing of cells of another organism
E	0035821	biological_process	modulation of process of another organism
E	0042151	cellular_component	nematocyst
E	0042802	molecular_function	identical protein binding
E	0044218	cellular_component	other organism cell membrane
E	0046930	cellular_component	pore complex
E	0046931	biological_process	pore complex assembly
E	0051715	biological_process	cytolysis in another organism
E	0055085	biological_process	transmembrane transport
E	0090729	molecular_function	toxin activity
F	0005576	cellular_component	extracellular region
F	0006811	biological_process	monoatomic ion transport
F	0006812	biological_process	monoatomic cation transport
F	0008289	molecular_function	lipid binding
F	0015267	molecular_function	channel activity
F	0016020	cellular_component	membrane
F	0031640	biological_process	killing of cells of another organism
F	0035821	biological_process	modulation of process of another organism
F	0042151	cellular_component	nematocyst
F	0042802	molecular_function	identical protein binding
F	0044218	cellular_component	other organism cell membrane
F	0046930	cellular_component	pore complex
F	0046931	biological_process	pore complex assembly
F	0051715	biological_process	cytolysis in another organism
F	0055085	biological_process	transmembrane transport
F	0090729	molecular_function	toxin activity
G	0005576	cellular_component	extracellular region
G	0006811	biological_process	monoatomic ion transport
G	0006812	biological_process	monoatomic cation transport
G	0008289	molecular_function	lipid binding
G	0015267	molecular_function	channel activity
G	0016020	cellular_component	membrane
G	0031640	biological_process	killing of cells of another organism
G	0035821	biological_process	modulation of process of another organism
G	0042151	cellular_component	nematocyst
G	0042802	molecular_function	identical protein binding
G	0044218	cellular_component	other organism cell membrane
G	0046930	cellular_component	pore complex
G	0046931	biological_process	pore complex assembly
G	0051715	biological_process	cytolysis in another organism
G	0055085	biological_process	transmembrane transport
G	0090729	molecular_function	toxin activity
H	0005576	cellular_component	extracellular region
H	0006811	biological_process	monoatomic ion transport
H	0006812	biological_process	monoatomic cation transport
H	0008289	molecular_function	lipid binding
H	0015267	molecular_function	channel activity
H	0016020	cellular_component	membrane
H	0031640	biological_process	killing of cells of another organism
H	0035821	biological_process	modulation of process of another organism
H	0042151	cellular_component	nematocyst
H	0042802	molecular_function	identical protein binding
H	0044218	cellular_component	other organism cell membrane
H	0046930	cellular_component	pore complex
H	0046931	biological_process	pore complex assembly
H	0051715	biological_process	cytolysis in another organism
H	0055085	biological_process	transmembrane transport
H	0090729	molecular_function	toxin activity
I	0005576	cellular_component	extracellular region
I	0006811	biological_process	monoatomic ion transport
I	0006812	biological_process	monoatomic cation transport
I	0008289	molecular_function	lipid binding
I	0015267	molecular_function	channel activity
I	0016020	cellular_component	membrane
I	0031640	biological_process	killing of cells of another organism
I	0035821	biological_process	modulation of process of another organism
I	0042151	cellular_component	nematocyst
I	0042802	molecular_function	identical protein binding
I	0044218	cellular_component	other organism cell membrane
I	0046930	cellular_component	pore complex
I	0046931	biological_process	pore complex assembly
I	0051715	biological_process	cytolysis in another organism
I	0055085	biological_process	transmembrane transport
I	0090729	molecular_function	toxin activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	18
Details	BINDING: in subunit A; in oligomeric forms only => ECO:0000305|PubMed:25716479

Chain	Residue	Details
A	ARG31
E	GLY168
F	ARG31
F	GLY168
G	ARG31
G	GLY168
H	ARG31
H	GLY168
I	ARG31
I	GLY168
A	GLY168
B	ARG31
B	GLY168
C	ARG31
C	GLY168
D	ARG31
D	GLY168
E	ARG31

---

site_id	SWS_FT_FI2
Number of Residues	27
Details	BINDING: BINDING => ECO:0000269|PubMed:28630155

Chain	Residue	Details
A	TYR51
D	TYR51
D	ARG53
D	TYR138
E	TYR51
E	ARG53
E	TYR138
F	TYR51
F	ARG53
F	TYR138
G	TYR51
A	ARG53
G	ARG53
G	TYR138
H	TYR51
H	ARG53
H	TYR138
I	TYR51
I	ARG53
I	TYR138
A	TYR138
B	TYR51
B	ARG53
B	TYR138
C	TYR51
C	ARG53
C	TYR138

---

site_id	SWS_FT_FI3
Number of Residues	81
Details	BINDING: in monomeric and oligomeric forms => ECO:0000305|PubMed:25716479

Chain	Residue	Details
A	SER54
B	SER54
B	GLY85
B	TYR108
B	TYR113
B	SER114
B	TRP116
B	TYR133
B	TYR137
B	ARG144
C	SER54
A	GLY85
C	GLY85
C	TYR108
C	TYR113
C	SER114
C	TRP116
C	TYR133
C	TYR137
C	ARG144
D	SER54
D	GLY85
A	TYR108
D	TYR108
D	TYR113
D	SER114
D	TRP116
D	TYR133
D	TYR137
D	ARG144
E	SER54
E	GLY85
E	TYR108
A	TYR113
E	TYR113
E	SER114
E	TRP116
E	TYR133
E	TYR137
E	ARG144
F	SER54
F	GLY85
F	TYR108
F	TYR113
A	SER114
F	SER114
F	TRP116
F	TYR133
F	TYR137
F	ARG144
G	SER54
G	GLY85
G	TYR108
G	TYR113
G	SER114
A	TRP116
G	TRP116
G	TYR133
G	TYR137
G	ARG144
H	SER54
H	GLY85
H	TYR108
H	TYR113
H	SER114
H	TRP116
A	TYR133
H	TYR133
H	TYR137
H	ARG144
I	SER54
I	GLY85
I	TYR108
I	TYR113
I	SER114
I	TRP116
I	TYR133
A	TYR137
I	TYR137
I	ARG144
A	ARG144

---

site_id	SWS_FT_FI4
Number of Residues	9
Details	BINDING: in subunit B; in oligomeric forms only => ECO:0000305|PubMed:25716479

Chain	Residue	Details
A	ARG79
B	ARG79
C	ARG79
D	ARG79
E	ARG79
F	ARG79
G	ARG79
H	ARG79
I	ARG79

---

site_id	SWS_FT_FI5
Number of Residues	9
Details	SITE: Part of the hydrophobic cavity (in subunit A) that receives Val-60 from the adjacent subunit (B); essential in hemolysis, since it is critical for pore formation in cholesterol-rich membrane cells (such as red blood cells) => ECO:0000305|PubMed:21300287, ECO:0000305|PubMed:25716479, ECO:0000305|PubMed:25759390

Chain	Residue	Details
A	PHE16
B	PHE16
C	PHE16
D	PHE16
E	PHE16
F	PHE16
G	PHE16
H	PHE16
I	PHE16

---

site_id	SWS_FT_FI6
Number of Residues	9
Details	SITE: Protrudes from one subunit (B) and inserts into the hydrophobic cavity from the adjacent subunit (A) => ECO:0000305|PubMed:21300287, ECO:0000305|PubMed:25716479

Chain	Residue	Details
A	VAL60
B	VAL60
C	VAL60
D	VAL60
E	VAL60
F	VAL60
G	VAL60
H	VAL60
I	VAL60

---

site_id	SWS_FT_FI7
Number of Residues	9
Details	SITE: Part of the hydrophobic cavity (in subunit A) that receives Val-60 from the adjacent subunit (B) => ECO:0000305|PubMed:21300287, ECO:0000305|PubMed:25716479

Chain	Residue	Details
A	TRP149
B	TRP149
C	TRP149
D	TRP149
E	TRP149
F	TRP149
G	TRP149
H	TRP149
I	TRP149

---

site_id	SWS_FT_FI8
Number of Residues	9
Details	SITE: Part of the hydrophobic cavity (in subunit A) that receives Val-60 from the adjacent subunit (B) => ECO:0000305|PubMed:21300287

Chain	Residue	Details
A	PHE163
B	PHE163
C	PHE163
D	PHE163
E	PHE163
F	PHE163
G	PHE163
H	PHE163
I	PHE163

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