3ZWG

Crystal structure of the pore-forming toxin FraC from Actinia fragacea (form 2)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005576	cellular_component	extracellular region
A	0006811	biological_process	monoatomic ion transport
A	0006812	biological_process	monoatomic cation transport
A	0008289	molecular_function	lipid binding
A	0015267	molecular_function	channel activity
A	0031640	biological_process	killing of cells of another organism
A	0035821	biological_process	modulation of process of another organism
A	0042151	cellular_component	nematocyst
A	0042802	molecular_function	identical protein binding
A	0044218	cellular_component	other organism cell membrane
A	0046930	cellular_component	pore complex
A	0046931	biological_process	pore complex assembly
A	0051715	biological_process	cytolysis in another organism
A	0055085	biological_process	transmembrane transport
A	0090729	molecular_function	toxin activity
B	0005576	cellular_component	extracellular region
B	0006811	biological_process	monoatomic ion transport
B	0006812	biological_process	monoatomic cation transport
B	0008289	molecular_function	lipid binding
B	0015267	molecular_function	channel activity
B	0031640	biological_process	killing of cells of another organism
B	0035821	biological_process	modulation of process of another organism
B	0042151	cellular_component	nematocyst
B	0042802	molecular_function	identical protein binding
B	0044218	cellular_component	other organism cell membrane
B	0046930	cellular_component	pore complex
B	0046931	biological_process	pore complex assembly
B	0051715	biological_process	cytolysis in another organism
B	0055085	biological_process	transmembrane transport
B	0090729	molecular_function	toxin activity
C	0005576	cellular_component	extracellular region
C	0006811	biological_process	monoatomic ion transport
C	0006812	biological_process	monoatomic cation transport
C	0008289	molecular_function	lipid binding
C	0015267	molecular_function	channel activity
C	0031640	biological_process	killing of cells of another organism
C	0035821	biological_process	modulation of process of another organism
C	0042151	cellular_component	nematocyst
C	0042802	molecular_function	identical protein binding
C	0044218	cellular_component	other organism cell membrane
C	0046930	cellular_component	pore complex
C	0046931	biological_process	pore complex assembly
C	0051715	biological_process	cytolysis in another organism
C	0055085	biological_process	transmembrane transport
C	0090729	molecular_function	toxin activity
D	0005576	cellular_component	extracellular region
D	0006811	biological_process	monoatomic ion transport
D	0006812	biological_process	monoatomic cation transport
D	0008289	molecular_function	lipid binding
D	0015267	molecular_function	channel activity
D	0031640	biological_process	killing of cells of another organism
D	0035821	biological_process	modulation of process of another organism
D	0042151	cellular_component	nematocyst
D	0042802	molecular_function	identical protein binding
D	0044218	cellular_component	other organism cell membrane
D	0046930	cellular_component	pore complex
D	0046931	biological_process	pore complex assembly
D	0051715	biological_process	cytolysis in another organism
D	0055085	biological_process	transmembrane transport
D	0090729	molecular_function	toxin activity
E	0005576	cellular_component	extracellular region
E	0006811	biological_process	monoatomic ion transport
E	0006812	biological_process	monoatomic cation transport
E	0008289	molecular_function	lipid binding
E	0015267	molecular_function	channel activity
E	0031640	biological_process	killing of cells of another organism
E	0035821	biological_process	modulation of process of another organism
E	0042151	cellular_component	nematocyst
E	0042802	molecular_function	identical protein binding
E	0044218	cellular_component	other organism cell membrane
E	0046930	cellular_component	pore complex
E	0046931	biological_process	pore complex assembly
E	0051715	biological_process	cytolysis in another organism
E	0055085	biological_process	transmembrane transport
E	0090729	molecular_function	toxin activity
F	0005576	cellular_component	extracellular region
F	0006811	biological_process	monoatomic ion transport
F	0006812	biological_process	monoatomic cation transport
F	0008289	molecular_function	lipid binding
F	0015267	molecular_function	channel activity
F	0031640	biological_process	killing of cells of another organism
F	0035821	biological_process	modulation of process of another organism
F	0042151	cellular_component	nematocyst
F	0042802	molecular_function	identical protein binding
F	0044218	cellular_component	other organism cell membrane
F	0046930	cellular_component	pore complex
F	0046931	biological_process	pore complex assembly
F	0051715	biological_process	cytolysis in another organism
F	0055085	biological_process	transmembrane transport
F	0090729	molecular_function	toxin activity
G	0005576	cellular_component	extracellular region
G	0006811	biological_process	monoatomic ion transport
G	0006812	biological_process	monoatomic cation transport
G	0008289	molecular_function	lipid binding
G	0015267	molecular_function	channel activity
G	0031640	biological_process	killing of cells of another organism
G	0035821	biological_process	modulation of process of another organism
G	0042151	cellular_component	nematocyst
G	0042802	molecular_function	identical protein binding
G	0044218	cellular_component	other organism cell membrane
G	0046930	cellular_component	pore complex
G	0046931	biological_process	pore complex assembly
G	0051715	biological_process	cytolysis in another organism
G	0055085	biological_process	transmembrane transport
G	0090729	molecular_function	toxin activity
H	0005576	cellular_component	extracellular region
H	0006811	biological_process	monoatomic ion transport
H	0006812	biological_process	monoatomic cation transport
H	0008289	molecular_function	lipid binding
H	0015267	molecular_function	channel activity
H	0031640	biological_process	killing of cells of another organism
H	0035821	biological_process	modulation of process of another organism
H	0042151	cellular_component	nematocyst
H	0042802	molecular_function	identical protein binding
H	0044218	cellular_component	other organism cell membrane
H	0046930	cellular_component	pore complex
H	0046931	biological_process	pore complex assembly
H	0051715	biological_process	cytolysis in another organism
H	0055085	biological_process	transmembrane transport
H	0090729	molecular_function	toxin activity
I	0005576	cellular_component	extracellular region
I	0006811	biological_process	monoatomic ion transport
I	0006812	biological_process	monoatomic cation transport
I	0008289	molecular_function	lipid binding
I	0015267	molecular_function	channel activity
I	0031640	biological_process	killing of cells of another organism
I	0035821	biological_process	modulation of process of another organism
I	0042151	cellular_component	nematocyst
I	0042802	molecular_function	identical protein binding
I	0044218	cellular_component	other organism cell membrane
I	0046930	cellular_component	pore complex
I	0046931	biological_process	pore complex assembly
I	0051715	biological_process	cytolysis in another organism
I	0055085	biological_process	transmembrane transport
I	0090729	molecular_function	toxin activity
J	0005576	cellular_component	extracellular region
J	0006811	biological_process	monoatomic ion transport
J	0006812	biological_process	monoatomic cation transport
J	0008289	molecular_function	lipid binding
J	0015267	molecular_function	channel activity
J	0031640	biological_process	killing of cells of another organism
J	0035821	biological_process	modulation of process of another organism
J	0042151	cellular_component	nematocyst
J	0042802	molecular_function	identical protein binding
J	0044218	cellular_component	other organism cell membrane
J	0046930	cellular_component	pore complex
J	0046931	biological_process	pore complex assembly
J	0051715	biological_process	cytolysis in another organism
J	0055085	biological_process	transmembrane transport
J	0090729	molecular_function	toxin activity
K	0005576	cellular_component	extracellular region
K	0006811	biological_process	monoatomic ion transport
K	0006812	biological_process	monoatomic cation transport
K	0008289	molecular_function	lipid binding
K	0015267	molecular_function	channel activity
K	0031640	biological_process	killing of cells of another organism
K	0035821	biological_process	modulation of process of another organism
K	0042151	cellular_component	nematocyst
K	0042802	molecular_function	identical protein binding
K	0044218	cellular_component	other organism cell membrane
K	0046930	cellular_component	pore complex
K	0046931	biological_process	pore complex assembly
K	0051715	biological_process	cytolysis in another organism
K	0055085	biological_process	transmembrane transport
K	0090729	molecular_function	toxin activity
L	0005576	cellular_component	extracellular region
L	0006811	biological_process	monoatomic ion transport
L	0006812	biological_process	monoatomic cation transport
L	0008289	molecular_function	lipid binding
L	0015267	molecular_function	channel activity
L	0031640	biological_process	killing of cells of another organism
L	0035821	biological_process	modulation of process of another organism
L	0042151	cellular_component	nematocyst
L	0042802	molecular_function	identical protein binding
L	0044218	cellular_component	other organism cell membrane
L	0046930	cellular_component	pore complex
L	0046931	biological_process	pore complex assembly
L	0051715	biological_process	cytolysis in another organism
L	0055085	biological_process	transmembrane transport
L	0090729	molecular_function	toxin activity
M	0005576	cellular_component	extracellular region
M	0006811	biological_process	monoatomic ion transport
M	0006812	biological_process	monoatomic cation transport
M	0008289	molecular_function	lipid binding
M	0015267	molecular_function	channel activity
M	0031640	biological_process	killing of cells of another organism
M	0035821	biological_process	modulation of process of another organism
M	0042151	cellular_component	nematocyst
M	0042802	molecular_function	identical protein binding
M	0044218	cellular_component	other organism cell membrane
M	0046930	cellular_component	pore complex
M	0046931	biological_process	pore complex assembly
M	0051715	biological_process	cytolysis in another organism
M	0055085	biological_process	transmembrane transport
M	0090729	molecular_function	toxin activity
N	0005576	cellular_component	extracellular region
N	0006811	biological_process	monoatomic ion transport
N	0006812	biological_process	monoatomic cation transport
N	0008289	molecular_function	lipid binding
N	0015267	molecular_function	channel activity
N	0031640	biological_process	killing of cells of another organism
N	0035821	biological_process	modulation of process of another organism
N	0042151	cellular_component	nematocyst
N	0042802	molecular_function	identical protein binding
N	0044218	cellular_component	other organism cell membrane
N	0046930	cellular_component	pore complex
N	0046931	biological_process	pore complex assembly
N	0051715	biological_process	cytolysis in another organism
N	0055085	biological_process	transmembrane transport
N	0090729	molecular_function	toxin activity
O	0005576	cellular_component	extracellular region
O	0006811	biological_process	monoatomic ion transport
O	0006812	biological_process	monoatomic cation transport
O	0008289	molecular_function	lipid binding
O	0015267	molecular_function	channel activity
O	0031640	biological_process	killing of cells of another organism
O	0035821	biological_process	modulation of process of another organism
O	0042151	cellular_component	nematocyst
O	0042802	molecular_function	identical protein binding
O	0044218	cellular_component	other organism cell membrane
O	0046930	cellular_component	pore complex
O	0046931	biological_process	pore complex assembly
O	0051715	biological_process	cytolysis in another organism
O	0055085	biological_process	transmembrane transport
O	0090729	molecular_function	toxin activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	225
Details	Region: {"description":"Trp-rich region, which is important for the binding to lipid membrane","evidences":[{"source":"UniProtKB","id":"P61914","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	30
Details	Motif: {"description":"Cell attachment site, crucial for protein stability","evidences":[{"source":"UniProtKB","id":"P07845","evidenceCode":"ECO:0000250"},{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	30
Details	Binding site: {"description":"in subunit A; in oligomeric forms only","evidences":[{"source":"PubMed","id":"25716479","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	45
Details	Binding site: {"evidences":[{"source":"PubMed","id":"28630155","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	135
Details	Binding site: {"description":"in monomeric and oligomeric forms","evidences":[{"source":"PubMed","id":"25716479","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	15
Details	Binding site: {"description":"in subunit B; in oligomeric forms only","evidences":[{"source":"PubMed","id":"25716479","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	15
Details	Site: {"description":"Part of the hydrophobic cavity (in subunit A) that receives Val-60 from the adjacent subunit (B); essential in hemolysis, since it is critical for pore formation in cholesterol-rich membrane cells (such as red blood cells)","evidences":[{"source":"PubMed","id":"21300287","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"25716479","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"25759390","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	15
Details	Site: {"description":"Protrudes from one subunit (B) and inserts into the hydrophobic cavity from the adjacent subunit (A)","evidences":[{"source":"PubMed","id":"21300287","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"25716479","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	15
Details	Site: {"description":"Part of the hydrophobic cavity (in subunit A) that receives Val-60 from the adjacent subunit (B)","evidences":[{"source":"PubMed","id":"21300287","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"25716479","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	15
Details	Site: {"description":"Part of the hydrophobic cavity (in subunit A) that receives Val-60 from the adjacent subunit (B)","evidences":[{"source":"PubMed","id":"21300287","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---