Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3WWI

Crystal structure of the G136F mutant of the first R-stereoselective -transaminase identified from Arthrobacter sp. KNK168 (FERM-BP-5228)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004084	molecular_function	branched-chain-amino-acid transaminase activity
A	0005829	cellular_component	cytosol
A	0006532	biological_process	aspartate biosynthetic process
A	0009098	biological_process	L-leucine biosynthetic process
A	0009099	biological_process	valine biosynthetic process
A	0046394	biological_process	carboxylic acid biosynthetic process
B	0003824	molecular_function	catalytic activity
B	0004084	molecular_function	branched-chain-amino-acid transaminase activity
B	0005829	cellular_component	cytosol
B	0006532	biological_process	aspartate biosynthetic process
B	0009098	biological_process	L-leucine biosynthetic process
B	0009099	biological_process	valine biosynthetic process
B	0046394	biological_process	carboxylic acid biosynthetic process
C	0003824	molecular_function	catalytic activity
C	0004084	molecular_function	branched-chain-amino-acid transaminase activity
C	0005829	cellular_component	cytosol
C	0006532	biological_process	aspartate biosynthetic process
C	0009098	biological_process	L-leucine biosynthetic process
C	0009099	biological_process	valine biosynthetic process
C	0046394	biological_process	carboxylic acid biosynthetic process
D	0003824	molecular_function	catalytic activity
D	0004084	molecular_function	branched-chain-amino-acid transaminase activity
D	0005829	cellular_component	cytosol
D	0006532	biological_process	aspartate biosynthetic process
D	0009098	biological_process	L-leucine biosynthetic process
D	0009099	biological_process	valine biosynthetic process
D	0046394	biological_process	carboxylic acid biosynthetic process
E	0003824	molecular_function	catalytic activity
E	0004084	molecular_function	branched-chain-amino-acid transaminase activity
E	0005829	cellular_component	cytosol
E	0006532	biological_process	aspartate biosynthetic process
E	0009098	biological_process	L-leucine biosynthetic process
E	0009099	biological_process	valine biosynthetic process
E	0046394	biological_process	carboxylic acid biosynthetic process
F	0003824	molecular_function	catalytic activity
F	0004084	molecular_function	branched-chain-amino-acid transaminase activity
F	0005829	cellular_component	cytosol
F	0006532	biological_process	aspartate biosynthetic process
F	0009098	biological_process	L-leucine biosynthetic process
F	0009099	biological_process	valine biosynthetic process
F	0046394	biological_process	carboxylic acid biosynthetic process
G	0003824	molecular_function	catalytic activity
G	0004084	molecular_function	branched-chain-amino-acid transaminase activity
G	0005829	cellular_component	cytosol
G	0006532	biological_process	aspartate biosynthetic process
G	0009098	biological_process	L-leucine biosynthetic process
G	0009099	biological_process	valine biosynthetic process
G	0046394	biological_process	carboxylic acid biosynthetic process
H	0003824	molecular_function	catalytic activity
H	0004084	molecular_function	branched-chain-amino-acid transaminase activity
H	0005829	cellular_component	cytosol
H	0006532	biological_process	aspartate biosynthetic process
H	0009098	biological_process	L-leucine biosynthetic process
H	0009099	biological_process	valine biosynthetic process
H	0046394	biological_process	carboxylic acid biosynthetic process
I	0003824	molecular_function	catalytic activity
I	0004084	molecular_function	branched-chain-amino-acid transaminase activity
I	0005829	cellular_component	cytosol
I	0006532	biological_process	aspartate biosynthetic process
I	0009098	biological_process	L-leucine biosynthetic process
I	0009099	biological_process	valine biosynthetic process
I	0046394	biological_process	carboxylic acid biosynthetic process
J	0003824	molecular_function	catalytic activity
J	0004084	molecular_function	branched-chain-amino-acid transaminase activity
J	0005829	cellular_component	cytosol
J	0006532	biological_process	aspartate biosynthetic process
J	0009098	biological_process	L-leucine biosynthetic process
J	0009099	biological_process	valine biosynthetic process
J	0046394	biological_process	carboxylic acid biosynthetic process
K	0003824	molecular_function	catalytic activity
K	0004084	molecular_function	branched-chain-amino-acid transaminase activity
K	0005829	cellular_component	cytosol
K	0006532	biological_process	aspartate biosynthetic process
K	0009098	biological_process	L-leucine biosynthetic process
K	0009099	biological_process	valine biosynthetic process
K	0046394	biological_process	carboxylic acid biosynthetic process
L	0003824	molecular_function	catalytic activity
L	0004084	molecular_function	branched-chain-amino-acid transaminase activity
L	0005829	cellular_component	cytosol
L	0006532	biological_process	aspartate biosynthetic process
L	0009098	biological_process	L-leucine biosynthetic process
L	0009099	biological_process	valine biosynthetic process
L	0046394	biological_process	carboxylic acid biosynthetic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	binding site for residue PLP A 401

Chain	Residue
A	ARG86
A	THR282
A	THR283
A	HOH517
A	HOH519
A	HOH528
A	HOH543
A	LYS188
A	GLU221
A	PHE225
A	ASN226
A	LEU243
A	GLY245
A	ILE246
A	THR247

site_id	AC2
Number of Residues	19
Details	binding site for Di-peptide PLP B 401 and LYS B 188

Chain	Residue
B	THR66
B	TYR67
B	ARG86
B	ASN90
B	VAL187
B	ASN189
B	GLU221
B	GLY224
B	PHE225
B	ASN226
B	LEU243
B	GLY245
B	ILE246
B	THR247
B	THR282
B	THR283
B	HOH506
B	HOH530
B	HOH532

site_id	AC3
Number of Residues	18
Details	binding site for Di-peptide PLP C 401 and LYS C 188

Chain	Residue
C	THR66
C	TYR67
C	ARG86
C	ASN90
C	VAL187
C	ASN189
C	GLU221
C	PHE225
C	ASN226
C	LEU243
C	GLY245
C	ILE246
C	THR247
C	THR282
C	THR283
C	HOH513
C	HOH515
C	HOH587

site_id	AC4
Number of Residues	19
Details	binding site for Di-peptide PLP D 401 and LYS D 188

Chain	Residue
D	THR66
D	TYR67
D	ARG86
D	ASN90
D	VAL187
D	ASN189
D	GLU221
D	GLY224
D	PHE225
D	ASN226
D	LEU243
D	GLY245
D	ILE246
D	THR247
D	THR282
D	THR283
D	HOH503
D	HOH512
D	HOH519

site_id	AC5
Number of Residues	18
Details	binding site for Di-peptide PLP E 401 and LYS E 188

Chain	Residue
E	THR66
E	TYR67
E	ARG86
E	ASN90
E	VAL187
E	ASN189
E	GLU221
E	PHE225
E	ASN226
E	LEU243
E	GLY245
E	ILE246
E	THR247
E	THR282
E	THR283
E	HOH515
E	HOH522
E	HOH578

site_id	AC6
Number of Residues	18
Details	binding site for Di-peptide PLP F 401 and LYS F 188

Chain	Residue
F	GLY245
F	ILE246
F	THR247
F	THR282
F	THR283
F	HOH512
F	HOH515
F	HOH539
F	THR66
F	TYR67
F	ARG86
F	ASN90
F	VAL187
F	ASN189
F	GLU221
F	PHE225
F	ASN226
F	LEU243

site_id	AC7
Number of Residues	19
Details	binding site for Di-peptide PLP G 401 and LYS G 188

Chain	Residue
G	THR66
G	TYR67
G	ARG86
G	ASN90
G	VAL187
G	ASN189
G	GLU221
G	GLY224
G	PHE225
G	ASN226
G	LEU243
G	GLY245
G	ILE246
G	THR247
G	THR282
G	THR283
G	HOH507
G	HOH508
G	HOH521

site_id	AC8
Number of Residues	18
Details	binding site for Di-peptide PLP H 401 and LYS H 188

Chain	Residue
H	THR66
H	TYR67
H	ARG86
H	ASN90
H	VAL187
H	ASN189
H	GLU221
H	GLY224
H	PHE225
H	LEU243
H	GLY245
H	ILE246
H	THR247
H	THR282
H	THR283
H	HOH501
H	HOH517
H	HOH582

site_id	AC9
Number of Residues	19
Details	binding site for Di-peptide PLP I 401 and LYS I 188

Chain	Residue
I	THR66
I	TYR67
I	ARG86
I	ASN90
I	VAL187
I	ASN189
I	GLU221
I	GLY224
I	PHE225
I	ASN226
I	LEU243
I	GLY245
I	ILE246
I	THR247
I	THR282
I	THR283
I	HOH503
I	HOH527
I	HOH565

site_id	AD1
Number of Residues	19
Details	binding site for Di-peptide PLP J 401 and LYS J 188

Chain	Residue
J	THR66
J	TYR67
J	ARG86
J	ASN90
J	VAL187
J	ASN189
J	GLU221
J	GLY224
J	PHE225
J	ASN226
J	LEU243
J	GLY245
J	ILE246
J	THR247
J	THR282
J	THR283
J	HOH504
J	HOH510
J	HOH534

site_id	AD2
Number of Residues	18
Details	binding site for Di-peptide PLP K 401 and LYS K 188

Chain	Residue
K	THR66
K	TYR67
K	ARG86
K	ASN90
K	VAL187
K	ASN189
K	GLU221
K	PHE225
K	ASN226
K	LEU243
K	GLY245
K	ILE246
K	THR247
K	THR282
K	THR283
K	HOH515
K	HOH517
K	HOH545

site_id	AD3
Number of Residues	18
Details	binding site for Di-peptide PLP L 401 and LYS L 188

Chain	Residue
L	THR66
L	TYR67
L	ARG86
L	ASN90
L	VAL187
L	ASN189
L	GLU221
L	GLY224
L	PHE225
L	ASN226
L	LEU243
L	GLY245
L	ILE246
L	THR247
L	THR282
L	THR283
L	HOH503
L	HOH531

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)