3WBE

Rice Os3BGlu6 Beta-Glucosidase E178Q mutant in a covalent complex with Glc from GA4GE.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
A	0005975	biological_process	carbohydrate metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	12
Details

Chain	Residue
A	GLN31
A	TRP452
A	PHE460
A	GOL504
A	HIS132
A	ASN177
A	GLN178
A	TYR321
A	TRP366
A	GLU394
A	TRP444
A	GLU451

---

site_id	AC2
Number of Residues	9
Details

Chain	Residue
A	GLN31
A	HIS132
A	ASN177
A	GLN178
A	GLU394
A	TRP444
A	GLU451
A	TRP452
A	GOL504

---

Functional Information from PROSITE/UniProt

site_id	PS00653
Number of Residues	15
Details	GLYCOSYL_HYDROL_F1_2 Glycosyl hydrolases family 1 N-terminal signature. FvFGtAsAAYQyEgA

Chain	Residue	Details
A	PHE21-ALA35

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000269|PubMed:23811195

Chain	Residue	Details
A	GLN178

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:23811195

Chain	Residue	Details
A	GLU394

---

site_id	SWS_FT_FI3
Number of Residues	5
Details	BINDING: BINDING => ECO:0000305|PubMed:19587102, ECO:0000305|PubMed:23811195, ECO:0007744|PDB:3GNP, ECO:0007744|PDB:3WBA

Chain	Residue	Details
A	GLN31
A	ASN177
A	TYR321
A	TRP444
A	GLU451

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	BINDING: BINDING => ECO:0000305|PubMed:23811195, ECO:0007744|PDB:3WBA

Chain	Residue	Details
A	HIS132

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	BINDING: BINDING => ECO:0000250|UniProtKB:Q9SPP9

Chain	Residue	Details
A	GLU394

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	BINDING: BINDING => ECO:0000250|UniProtKB:Q1XH05

Chain	Residue	Details
A	PHE460

---

site_id	SWS_FT_FI7
Number of Residues	3
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255|PROSITE-ProRule:PRU00498

Chain	Residue	Details
A	ASN258
A	ASN329
A	ASN339

---