3VZL
Crystal structure of the Bacillus circulans endo-beta-(1,4)-xylanase (BcX) N35H mutant
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000272 | biological_process | polysaccharide catabolic process |
| A | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| A | 0031176 | molecular_function | endo-1,4-beta-xylanase activity |
| A | 0045493 | biological_process | xylan catabolic process |
| B | 0000272 | biological_process | polysaccharide catabolic process |
| B | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| B | 0005975 | biological_process | carbohydrate metabolic process |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| B | 0031176 | molecular_function | endo-1,4-beta-xylanase activity |
| B | 0045493 | biological_process | xylan catabolic process |
| C | 0000272 | biological_process | polysaccharide catabolic process |
| C | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| C | 0005975 | biological_process | carbohydrate metabolic process |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| C | 0031176 | molecular_function | endo-1,4-beta-xylanase activity |
| C | 0045493 | biological_process | xylan catabolic process |
| D | 0000272 | biological_process | polysaccharide catabolic process |
| D | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| D | 0005975 | biological_process | carbohydrate metabolic process |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| D | 0031176 | molecular_function | endo-1,4-beta-xylanase activity |
| D | 0045493 | biological_process | xylan catabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 B 201 |
| Chain | Residue |
| B | THR123 |
| B | THR124 |
| C | SER100 |
| C | ASP101 |
| C | ASN148 |
| C | HOH357 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 C 201 |
| Chain | Residue |
| C | TYR94 |
| C | GLY96 |
| C | THR97 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 D 201 |
| Chain | Residue |
| D | SER100 |
| D | ASP101 |
| D | ASN148 |
| D | HOH322 |
| D | HOH348 |
Functional Information from PROSITE/UniProt
| site_id | PS00776 |
| Number of Residues | 11 |
| Details | GH11_1 Glycosyl hydrolases family 11 (GH11) active site signature 1. PLiEYYVVDsW |
| Chain | Residue | Details |
| A | PRO75-TRP85 |
| site_id | PS00777 |
| Number of Residues | 12 |
| Details | GH11_2 Glycosyl hydrolases family 11 (GH11) active site signature 2. MatEGYQSSGsS |
| Chain | Residue | Details |
| A | MET169-SER180 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 736 |
| Details | Domain: {"description":"GH11","evidences":[{"source":"PROSITE-ProRule","id":"PRU01097","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Nucleophile","evidences":[{"source":"PROSITE-ProRule","id":"PRU10062","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"8019418","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Proton donor","evidences":[{"source":"PROSITE-ProRule","id":"PRU10063","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"8019418","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 432 |
| Chain | Residue | Details |
| A | HIS35 | modifies pKa |
| A | TYR69 | electrostatic destabiliser |
| A | GLU78 | covalent catalysis, proton shuttle (general acid/base) |
| A | TYR80 | modifies pKa |
| A | GLU172 | proton shuttle (general acid/base) |
| site_id | MCSA2 |
| Number of Residues | 5 |
| Details | M-CSA 432 |
| Chain | Residue | Details |
| B | HIS35 | modifies pKa |
| B | TYR69 | electrostatic destabiliser |
| B | GLU78 | covalent catalysis, proton shuttle (general acid/base) |
| B | TYR80 | modifies pKa |
| B | GLU172 | proton shuttle (general acid/base) |
| site_id | MCSA3 |
| Number of Residues | 5 |
| Details | M-CSA 432 |
| Chain | Residue | Details |
| C | HIS35 | modifies pKa |
| C | TYR69 | electrostatic destabiliser |
| C | GLU78 | covalent catalysis, proton shuttle (general acid/base) |
| C | TYR80 | modifies pKa |
| C | GLU172 | proton shuttle (general acid/base) |
| site_id | MCSA4 |
| Number of Residues | 5 |
| Details | M-CSA 432 |
| Chain | Residue | Details |
| D | HIS35 | modifies pKa |
| D | TYR69 | electrostatic destabiliser |
| D | GLU78 | covalent catalysis, proton shuttle (general acid/base) |
| D | TYR80 | modifies pKa |
| D | GLU172 | proton shuttle (general acid/base) |
