3VD5
E. coli (lacZ) beta-galactosidase (N460S)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| A | 0004565 | molecular_function | beta-galactosidase activity |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0005990 | biological_process | lactose catabolic process |
| A | 0009056 | biological_process | catabolic process |
| A | 0009341 | cellular_component | beta-galactosidase complex |
| A | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| A | 0030246 | molecular_function | carbohydrate binding |
| A | 0031420 | molecular_function | alkali metal ion binding |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| B | 0004565 | molecular_function | beta-galactosidase activity |
| B | 0005975 | biological_process | carbohydrate metabolic process |
| B | 0005990 | biological_process | lactose catabolic process |
| B | 0009056 | biological_process | catabolic process |
| B | 0009341 | cellular_component | beta-galactosidase complex |
| B | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| B | 0030246 | molecular_function | carbohydrate binding |
| B | 0031420 | molecular_function | alkali metal ion binding |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0046872 | molecular_function | metal ion binding |
| C | 0000287 | molecular_function | magnesium ion binding |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| C | 0004565 | molecular_function | beta-galactosidase activity |
| C | 0005975 | biological_process | carbohydrate metabolic process |
| C | 0005990 | biological_process | lactose catabolic process |
| C | 0009056 | biological_process | catabolic process |
| C | 0009341 | cellular_component | beta-galactosidase complex |
| C | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| C | 0030246 | molecular_function | carbohydrate binding |
| C | 0031420 | molecular_function | alkali metal ion binding |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0046872 | molecular_function | metal ion binding |
| D | 0000287 | molecular_function | magnesium ion binding |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| D | 0004565 | molecular_function | beta-galactosidase activity |
| D | 0005975 | biological_process | carbohydrate metabolic process |
| D | 0005990 | biological_process | lactose catabolic process |
| D | 0009056 | biological_process | catabolic process |
| D | 0009341 | cellular_component | beta-galactosidase complex |
| D | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| D | 0030246 | molecular_function | carbohydrate binding |
| D | 0031420 | molecular_function | alkali metal ion binding |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG A 3001 |
| Chain | Residue |
| A | GLU416 |
| A | HIS418 |
| A | GLU461 |
| A | HOH4041 |
| A | HOH4194 |
| A | HOH4195 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG A 3002 |
| Chain | Residue |
| A | GLN163 |
| A | ASP193 |
| A | ASP15 |
| A | ASN18 |
| A | VAL21 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA A 3101 |
| Chain | Residue |
| A | ASP201 |
| A | PHE601 |
| A | ASN604 |
| A | HOH4266 |
| A | HOH4267 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE NA A 3102 |
| Chain | Residue |
| A | PHE556 |
| A | TYR559 |
| A | PRO560 |
| A | LEU562 |
| A | HOH4112 |
| A | HOH4268 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE NA A 3103 |
| Chain | Residue |
| A | PRO932 |
| A | LEU967 |
| A | THR970 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE DMS A 8001 |
| Chain | Residue |
| A | LYS380 |
| A | ASN383 |
| A | PHE626 |
| A | TYR642 |
| A | TRP708 |
| A | HOH4275 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS A 8002 |
| Chain | Residue |
| A | ARG557 |
| A | HIS622 |
| A | GLN623 |
| A | GLN628 |
| site_id | AC8 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE DMS A 8003 |
| Chain | Residue |
| A | THR229 |
| A | VAL330 |
| A | GLY331 |
| A | ARG333 |
| A | ASN449 |
| A | PRO451 |
| A | ARG482 |
| site_id | AC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS A 8004 |
| Chain | Residue |
| A | GLY270 |
| A | THR271 |
| A | LEU291 |
| A | ARG292 |
| site_id | BC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS A 8005 |
| Chain | Residue |
| A | ASP45 |
| A | PRO47 |
| A | SER48 |
| A | DMS8007 |
| site_id | BC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS A 8006 |
| Chain | Residue |
| A | GLU57 |
| A | LEU125 |
| A | THR126 |
| C | GLU819 |
| site_id | BC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS A 8007 |
| Chain | Residue |
| A | PHE33 |
| A | ALA34 |
| A | ASP45 |
| A | ALA327 |
| A | DMS8005 |
| site_id | BC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG C 3001 |
| Chain | Residue |
| C | GLU416 |
| C | HIS418 |
| C | GLU461 |
| C | HOH4017 |
| C | HOH4109 |
| C | HOH4171 |
| site_id | BC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG C 3002 |
| Chain | Residue |
| C | ASP15 |
| C | ASN18 |
| C | VAL21 |
| C | GLN163 |
| C | ASP193 |
| site_id | BC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA C 3101 |
| Chain | Residue |
| C | TYR100 |
| C | ASP201 |
| C | PHE601 |
| C | ASN604 |
| C | HOH4173 |
| site_id | BC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA C 3102 |
| Chain | Residue |
| C | PHE556 |
| C | TYR559 |
| C | PRO560 |
| C | LEU562 |
| C | HOH4074 |
| site_id | BC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA C 3103 |
| Chain | Residue |
| C | PHE931 |
| C | PRO932 |
| C | LEU967 |
| C | THR970 |
| site_id | BC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DMS C 8001 |
| Chain | Residue |
| C | ILE576 |
| C | PRO584 |
| C | TRP585 |
| C | SER586 |
| C | ARG973 |
| site_id | CC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE DMS C 8002 |
| Chain | Residue |
| C | THR229 |
| C | GLY331 |
| C | ARG333 |
| C | PRO451 |
| C | ARG482 |
| C | HOH4081 |
| site_id | CC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS C 8003 |
| Chain | Residue |
| C | GLY275 |
| C | VAL289 |
| C | THR290 |
| site_id | CC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS C 8004 |
| Chain | Residue |
| C | GLN623 |
| C | GLN625 |
| C | GLN628 |
| C | HIS622 |
| site_id | CC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS C 8005 |
| Chain | Residue |
| C | PHE629 |
| C | ARG630 |
| C | HOH4107 |
| site_id | CC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG D 3001 |
| Chain | Residue |
| D | GLU416 |
| D | HIS418 |
| D | GLU461 |
| D | HOH4134 |
| D | HOH4231 |
| D | HOH4232 |
| site_id | CC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG D 3002 |
| Chain | Residue |
| D | ASP15 |
| D | ASN18 |
| D | VAL21 |
| D | GLN163 |
| D | ASP193 |
| site_id | CC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA D 3101 |
| Chain | Residue |
| D | TYR100 |
| D | ASP201 |
| D | PHE601 |
| D | ASN604 |
| D | HOH4233 |
| site_id | CC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA D 3102 |
| Chain | Residue |
| D | PHE556 |
| D | TYR559 |
| D | PRO560 |
| D | LEU562 |
| site_id | CC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS D 8001 |
| Chain | Residue |
| D | GLY275 |
| D | GLY276 |
| D | VAL289 |
| D | THR290 |
| site_id | DC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS D 8002 |
| Chain | Residue |
| D | GLY270 |
| D | THR290 |
| D | LEU291 |
| D | ARG292 |
| site_id | DC2 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE DMS D 8003 |
| Chain | Residue |
| D | ASP45 |
| site_id | DC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS D 8004 |
| Chain | Residue |
| D | LEU54 |
| D | ASN55 |
| D | GLU57 |
| D | LEU125 |
| site_id | DC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE DMS D 8005 |
| Chain | Residue |
| D | ARG557 |
| D | HIS622 |
| D | GLN623 |
| D | GLN628 |
| D | GLN718 |
| D | HOH4008 |
| D | HOH4041 |
| site_id | DC5 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE DMS D 8006 |
| Chain | Residue |
| D | ARG230 |
| site_id | DC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG B 3001 |
| Chain | Residue |
| B | GLU416 |
| B | HIS418 |
| B | GLU461 |
| B | HOH4027 |
| B | HOH4111 |
| B | HOH4211 |
| site_id | DC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG B 3002 |
| Chain | Residue |
| B | ASP15 |
| B | ASN18 |
| B | VAL21 |
| B | GLN163 |
| B | ASP193 |
| site_id | DC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE NA B 3101 |
| Chain | Residue |
| B | TYR100 |
| B | ASP201 |
| B | PHE601 |
| B | ASN604 |
| B | HOH4244 |
| B | HOH4245 |
| site_id | DC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA B 3102 |
| Chain | Residue |
| B | PHE556 |
| B | TYR559 |
| B | PRO560 |
| B | LEU562 |
| B | HOH4217 |
| site_id | EC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA B 3103 |
| Chain | Residue |
| B | SER647 |
| B | GLU650 |
| B | LEU670 |
| B | HOH4013 |
| B | HOH4155 |
| site_id | EC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE DMS B 8001 |
| Chain | Residue |
| B | LEU291 |
| B | ARG292 |
| site_id | EC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE DMS B 8002 |
| Chain | Residue |
| B | LYS380 |
| B | ASN383 |
| B | PHE384 |
| B | PHE626 |
| B | TYR642 |
| B | TRP708 |
| site_id | EC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DMS B 8003 |
| Chain | Residue |
| B | VAL335 |
| B | PRO480 |
| B | SER481 |
| site_id | EC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE DMS B 8004 |
| Chain | Residue |
| B | THR229 |
| B | VAL330 |
| B | GLY331 |
| B | ASN449 |
Functional Information from PROSITE/UniProt
| site_id | PS00719 |
| Number of Residues | 26 |
| Details | GLYCOSYL_HYDROL_F2_1 Glycosyl hydrolases family 2 signature 1. NaVRCSHYPnhplWYtlcDryGLYVV |
| Chain | Residue | Details |
| A | ASN385-VAL410 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton donor => ECO:0000269|PubMed:6420154 |
| Chain | Residue | Details |
| A | GLU461 | |
| C | GLU461 | |
| D | GLU461 | |
| B | GLU461 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | ACT_SITE: Nucleophile => ECO:0000269|PubMed:1350782 |
| Chain | Residue | Details |
| A | GLU537 | |
| C | GLU537 | |
| D | GLU537 | |
| B | GLU537 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 28 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | ASN102 | |
| C | GLU461 | |
| C | GLU537 | |
| C | PHE601 | |
| C | ASN604 | |
| C | TRP999 | |
| D | ASN102 | |
| D | ASP201 | |
| D | GLU461 | |
| D | GLU537 | |
| D | PHE601 | |
| A | ASP201 | |
| D | ASN604 | |
| D | TRP999 | |
| B | ASN102 | |
| B | ASP201 | |
| B | GLU461 | |
| B | GLU537 | |
| B | PHE601 | |
| B | ASN604 | |
| B | TRP999 | |
| A | GLU461 | |
| A | GLU537 | |
| A | PHE601 | |
| A | ASN604 | |
| A | TRP999 | |
| C | ASN102 | |
| C | ASP201 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11045615 |
| Chain | Residue | Details |
| A | GLU416 | |
| B | GLU416 | |
| B | HIS418 | |
| B | ASN597 | |
| A | HIS418 | |
| A | ASN597 | |
| C | GLU416 | |
| C | HIS418 | |
| C | ASN597 | |
| D | GLU416 | |
| D | HIS418 | |
| D | ASN597 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 8 |
| Details | SITE: Transition state stabilizer |
| Chain | Residue | Details |
| A | HIS357 | |
| A | HIS391 | |
| C | HIS357 | |
| C | HIS391 | |
| D | HIS357 | |
| D | HIS391 | |
| B | HIS357 | |
| B | HIS391 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | SITE: Important for ensuring that an appropriate proportion of lactose is converted to allolactose |
| Chain | Residue | Details |
| A | TRP999 | |
| C | TRP999 | |
| D | TRP999 | |
| B | TRP999 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 10 |
| Details | M-CSA 422 |
| Chain | Residue | Details |
| A | ASP201 | |
| A | ASN604 | |
| A | HIS357 | |
| A | HIS391 | |
| A | GLU416 | |
| A | HIS418 | |
| A | GLU461 | |
| A | GLU537 | |
| A | ASN597 | |
| A | PHE601 |
| site_id | MCSA2 |
| Number of Residues | 10 |
| Details | M-CSA 422 |
| Chain | Residue | Details |
| C | ASP201 | |
| C | ASN604 | |
| C | HIS357 | |
| C | HIS391 | |
| C | GLU416 | |
| C | HIS418 | |
| C | GLU461 | |
| C | GLU537 | |
| C | ASN597 | |
| C | PHE601 |
| site_id | MCSA3 |
| Number of Residues | 10 |
| Details | M-CSA 422 |
| Chain | Residue | Details |
| D | ASP201 | |
| D | ASN604 | |
| D | HIS357 | |
| D | HIS391 | |
| D | GLU416 | |
| D | HIS418 | |
| D | GLU461 | |
| D | GLU537 | |
| D | ASN597 | |
| D | PHE601 |
| site_id | MCSA4 |
| Number of Residues | 10 |
| Details | M-CSA 422 |
| Chain | Residue | Details |
| B | ASP201 | |
| B | ASN604 | |
| B | HIS357 | |
| B | HIS391 | |
| B | GLU416 | |
| B | HIS418 | |
| B | GLU461 | |
| B | GLU537 | |
| B | ASN597 | |
| B | PHE601 |
