Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3V8E

Crystal structure of the yeast nicotinamidase Pnc1p bound to the inhibitor nicotinaldehyde

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000183	biological_process	rDNA heterochromatin formation
A	0000781	cellular_component	chromosome, telomeric region
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005777	cellular_component	peroxisome
A	0008270	molecular_function	zinc ion binding
A	0008936	molecular_function	nicotinamidase activity
A	0016787	molecular_function	hydrolase activity
A	0019358	biological_process	nicotinate nucleotide salvage
A	0019363	biological_process	pyridine nucleotide biosynthetic process
A	0031509	biological_process	subtelomeric heterochromatin formation
A	0046872	molecular_function	metal ion binding
A	1904524	biological_process	negative regulation of DNA amplification
B	0000183	biological_process	rDNA heterochromatin formation
B	0000781	cellular_component	chromosome, telomeric region
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005777	cellular_component	peroxisome
B	0008270	molecular_function	zinc ion binding
B	0008936	molecular_function	nicotinamidase activity
B	0016787	molecular_function	hydrolase activity
B	0019358	biological_process	nicotinate nucleotide salvage
B	0019363	biological_process	pyridine nucleotide biosynthetic process
B	0031509	biological_process	subtelomeric heterochromatin formation
B	0046872	molecular_function	metal ion binding
B	1904524	biological_process	negative regulation of DNA amplification
C	0000183	biological_process	rDNA heterochromatin formation
C	0000781	cellular_component	chromosome, telomeric region
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005777	cellular_component	peroxisome
C	0008270	molecular_function	zinc ion binding
C	0008936	molecular_function	nicotinamidase activity
C	0016787	molecular_function	hydrolase activity
C	0019358	biological_process	nicotinate nucleotide salvage
C	0019363	biological_process	pyridine nucleotide biosynthetic process
C	0031509	biological_process	subtelomeric heterochromatin formation
C	0046872	molecular_function	metal ion binding
C	1904524	biological_process	negative regulation of DNA amplification
D	0000183	biological_process	rDNA heterochromatin formation
D	0000781	cellular_component	chromosome, telomeric region
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005777	cellular_component	peroxisome
D	0008270	molecular_function	zinc ion binding
D	0008936	molecular_function	nicotinamidase activity
D	0016787	molecular_function	hydrolase activity
D	0019358	biological_process	nicotinate nucleotide salvage
D	0019363	biological_process	pyridine nucleotide biosynthetic process
D	0031509	biological_process	subtelomeric heterochromatin formation
D	0046872	molecular_function	metal ion binding
D	1904524	biological_process	negative regulation of DNA amplification
E	0000183	biological_process	rDNA heterochromatin formation
E	0000781	cellular_component	chromosome, telomeric region
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005777	cellular_component	peroxisome
E	0008270	molecular_function	zinc ion binding
E	0008936	molecular_function	nicotinamidase activity
E	0016787	molecular_function	hydrolase activity
E	0019358	biological_process	nicotinate nucleotide salvage
E	0019363	biological_process	pyridine nucleotide biosynthetic process
E	0031509	biological_process	subtelomeric heterochromatin formation
E	0046872	molecular_function	metal ion binding
E	1904524	biological_process	negative regulation of DNA amplification
F	0000183	biological_process	rDNA heterochromatin formation
F	0000781	cellular_component	chromosome, telomeric region
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005777	cellular_component	peroxisome
F	0008270	molecular_function	zinc ion binding
F	0008936	molecular_function	nicotinamidase activity
F	0016787	molecular_function	hydrolase activity
F	0019358	biological_process	nicotinate nucleotide salvage
F	0019363	biological_process	pyridine nucleotide biosynthetic process
F	0031509	biological_process	subtelomeric heterochromatin formation
F	0046872	molecular_function	metal ion binding
F	1904524	biological_process	negative regulation of DNA amplification
G	0000183	biological_process	rDNA heterochromatin formation
G	0000781	cellular_component	chromosome, telomeric region
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005777	cellular_component	peroxisome
G	0008270	molecular_function	zinc ion binding
G	0008936	molecular_function	nicotinamidase activity
G	0016787	molecular_function	hydrolase activity
G	0019358	biological_process	nicotinate nucleotide salvage
G	0019363	biological_process	pyridine nucleotide biosynthetic process
G	0031509	biological_process	subtelomeric heterochromatin formation
G	0046872	molecular_function	metal ion binding
G	1904524	biological_process	negative regulation of DNA amplification

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN A 300

Chain	Residue
A	ASP51
A	HIS53
A	HIS94
A	JJJ167
A	HOH449

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 301

Chain	Residue
A	HOH439
A	HOH440
A	PRO16
A	GLY18
A	THR21
A	HOH421

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN B 300

Chain	Residue
B	ASP51
B	HIS53
B	HIS94
B	JJJ167
B	HOH520
B	HOH527

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG B 301

Chain	Residue
B	PRO16
B	GLY18
B	THR21
B	HOH512
B	HOH513
B	HOH521

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN C 300

Chain	Residue
C	ASP51
C	HIS53
C	HIS94
C	JJJ167
C	HOH431

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG C 301

Chain	Residue
C	PRO16
C	GLY18
C	THR21
C	HOH423
C	HOH429
C	HOH430

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN D 300

Chain	Residue
D	ASP51
D	HIS53
D	HIS94
D	JJJ167
D	HOH425
D	HOH426

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG D 301

Chain	Residue
D	PRO16
D	GLY18
D	THR21
D	HOH414
D	HOH420

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN E 300

Chain	Residue
E	ASP51
E	HIS53
E	HIS94
E	JJJ167
E	HOH419

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG E 301

Chain	Residue
E	PRO16
E	GLY18
E	THR21
E	HOH414
E	HOH415

site_id	BC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN F 300

Chain	Residue
F	ASP51
F	HIS53
F	HIS94
F	GLU129
F	JJJ167
F	HOH412

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG F 301

Chain	Residue
F	PRO16
F	GLY18
F	THR21
F	HOH410
F	HOH411

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN G 300

Chain	Residue
G	ASP51
G	HIS53
G	HIS94
G	GLU129
G	JJJ167

site_id	BC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG G 301

Chain	Residue
B	HOH469
G	PRO16
G	GLY18
G	THR21
G	HOH409

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	14
Details	ACT_SITE: ACT_SITE => ECO:0000255

Chain	Residue	Details
A	ASP8
E	LYS122
F	ASP8
F	LYS122
G	ASP8
G	LYS122
A	LYS122
B	ASP8
B	LYS122
C	ASP8
C	LYS122
D	ASP8
D	LYS122
E	ASP8

site_id	SWS_FT_FI2
Number of Residues	7
Details	ACT_SITE: Nucleophile => ECO:0000255

Chain	Residue	Details
A	JJJ167
B	JJJ167
C	JJJ167
D	JJJ167
E	JJJ167
F	JJJ167
G	JJJ167

site_id	SWS_FT_FI3
Number of Residues	21
Details	BINDING: BINDING => ECO:0000269\|PubMed:17382284, ECO:0007744\|PDB:2H0R

Chain	Residue	Details
A	ASP51
D	ASP51
D	HIS53
D	HIS94
E	ASP51
E	HIS53
E	HIS94
F	ASP51
F	HIS53
F	HIS94
G	ASP51
A	HIS53
G	HIS53
G	HIS94
A	HIS94
B	ASP51
B	HIS53
B	HIS94
C	ASP51
C	HIS53
C	HIS94

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)