3UQD

Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with substrates and products

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003872	molecular_function	6-phosphofructokinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005829	cellular_component	cytosol
A	0005975	biological_process	carbohydrate metabolic process
A	0006096	biological_process	glycolytic process
A	0006974	biological_process	DNA damage response
A	0009024	molecular_function	tagatose-6-phosphate kinase activity
A	0016301	molecular_function	kinase activity
A	0016772	molecular_function	transferase activity, transferring phosphorus-containing groups
A	0016773	molecular_function	phosphotransferase activity, alcohol group as acceptor
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0046872	molecular_function	metal ion binding
A	0061615	biological_process	glycolytic process through fructose-6-phosphate
B	0000287	molecular_function	magnesium ion binding
B	0003872	molecular_function	6-phosphofructokinase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005829	cellular_component	cytosol
B	0005975	biological_process	carbohydrate metabolic process
B	0006096	biological_process	glycolytic process
B	0006974	biological_process	DNA damage response
B	0009024	molecular_function	tagatose-6-phosphate kinase activity
B	0016301	molecular_function	kinase activity
B	0016772	molecular_function	transferase activity, transferring phosphorus-containing groups
B	0016773	molecular_function	phosphotransferase activity, alcohol group as acceptor
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0046872	molecular_function	metal ion binding
B	0061615	biological_process	glycolytic process through fructose-6-phosphate
C	0000287	molecular_function	magnesium ion binding
C	0003872	molecular_function	6-phosphofructokinase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005829	cellular_component	cytosol
C	0005975	biological_process	carbohydrate metabolic process
C	0006096	biological_process	glycolytic process
C	0006974	biological_process	DNA damage response
C	0009024	molecular_function	tagatose-6-phosphate kinase activity
C	0016301	molecular_function	kinase activity
C	0016772	molecular_function	transferase activity, transferring phosphorus-containing groups
C	0016773	molecular_function	phosphotransferase activity, alcohol group as acceptor
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0046872	molecular_function	metal ion binding
C	0061615	biological_process	glycolytic process through fructose-6-phosphate
D	0000287	molecular_function	magnesium ion binding
D	0003872	molecular_function	6-phosphofructokinase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005829	cellular_component	cytosol
D	0005975	biological_process	carbohydrate metabolic process
D	0006096	biological_process	glycolytic process
D	0006974	biological_process	DNA damage response
D	0009024	molecular_function	tagatose-6-phosphate kinase activity
D	0016301	molecular_function	kinase activity
D	0016772	molecular_function	transferase activity, transferring phosphorus-containing groups
D	0016773	molecular_function	phosphotransferase activity, alcohol group as acceptor
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0046872	molecular_function	metal ion binding
D	0061615	biological_process	glycolytic process through fructose-6-phosphate

Functional Information from PROSITE/UniProt

site_id	PS00583
Number of Residues	25
Details	PFKB_KINASES_1 pfkB family of carbohydrate kinases signature 1. GGgGiNVAraIaHLGgsataifpaG

Chain	Residue	Details
A	GLY38-GLY62

---

site_id	PS00584
Number of Residues	14
Details	PFKB_KINASES_2 pfkB family of carbohydrate kinases signature 2. STvGAGDsmvGAMT

Chain	Residue	Details
A	SER250-THR263

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: ACT_SITE => ECO:0000250

Chain	Residue	Details
A	ASP256
B	ASP256
C	ASP256
D	ASP256

---

site_id	SWS_FT_FI2
Number of Residues	20
Details	BINDING:

Chain	Residue	Details
A	SER12
B	ASP256
C	SER12
C	GLY39
C	ARG90
C	SER139
C	ASP256
D	SER12
D	GLY39
D	ARG90
D	SER139
A	GLY39
D	ASP256
A	ARG90
A	SER139
A	ASP256
B	SER12
B	GLY39
B	ARG90
B	SER139

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:18762190, ECO:0007744|PDB:3CQD

Chain	Residue	Details
A	LYS27
B	LYS27
C	LYS27
D	LYS27

---

site_id	SWS_FT_FI4
Number of Residues	20
Details	BINDING: in other chain => ECO:0000269|PubMed:18762190, ECO:0000269|PubMed:23823238, ECO:0000269|Ref.10, ECO:0000269|Ref.11, ECO:0007744|PDB:3CQD

Chain	Residue	Details
A	LYS185
B	SER284
C	LYS185
C	SER224
C	SER248
C	VAL280
C	SER284
D	LYS185
D	SER224
D	SER248
D	VAL280
A	SER224
D	SER284
A	SER248
A	VAL280
A	SER284
B	LYS185
B	SER224
B	SER248
B	VAL280

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	BINDING: in other chain => ECO:0000269|PubMed:18762190, ECO:0007744|PDB:3CQD

Chain	Residue	Details
A	ASN187
B	ASN187
C	ASN187
D	ASN187

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	BINDING: BINDING => ECO:0000305

Chain	Residue	Details
A	GLU190
B	GLU190
C	GLU190
D	GLU190

---

site_id	SWS_FT_FI7
Number of Residues	24
Details	BINDING: BINDING => ECO:0000269|PubMed:23823238

Chain	Residue	Details
A	SER250
B	ASN289
B	GLY291
B	ARG293
C	SER250
C	VAL252
C	ALA286
C	ASN289
C	GLY291
C	ARG293
D	SER250
A	VAL252
D	VAL252
D	ALA286
D	ASN289
D	GLY291
D	ARG293
A	ALA286
A	ASN289
A	GLY291
A	ARG293
B	SER250
B	VAL252
B	ALA286

---