3UKO
Crystal Structure of S-Nitrosoglutathione Reductase from Arabidopsis thaliana, complex with NADH
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004022 | molecular_function | alcohol dehydrogenase (NAD+) activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005777 | cellular_component | peroxisome |
| A | 0005829 | cellular_component | cytosol |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0009536 | cellular_component | plastid |
| A | 0010286 | biological_process | heat acclimation |
| A | 0012501 | biological_process | programmed cell death |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0044281 | biological_process | small molecule metabolic process |
| A | 0046292 | biological_process | formaldehyde metabolic process |
| A | 0046294 | biological_process | formaldehyde catabolic process |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0048316 | biological_process | seed development |
| A | 0051903 | molecular_function | S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity |
| A | 0080007 | molecular_function | S-nitrosoglutathione reductase (NADH) activity |
| A | 0106321 | molecular_function | S-(hydroxymethyl)glutathione dehydrogenase (NADP+) activity |
| A | 0106322 | molecular_function | S-(hydroxymethyl)glutathione dehydrogenase (NAD+) activity |
| B | 0004022 | molecular_function | alcohol dehydrogenase (NAD+) activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005777 | cellular_component | peroxisome |
| B | 0005829 | cellular_component | cytosol |
| B | 0008270 | molecular_function | zinc ion binding |
| B | 0009536 | cellular_component | plastid |
| B | 0010286 | biological_process | heat acclimation |
| B | 0012501 | biological_process | programmed cell death |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0044281 | biological_process | small molecule metabolic process |
| B | 0046292 | biological_process | formaldehyde metabolic process |
| B | 0046294 | biological_process | formaldehyde catabolic process |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0048316 | biological_process | seed development |
| B | 0051903 | molecular_function | S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity |
| B | 0080007 | molecular_function | S-nitrosoglutathione reductase (NADH) activity |
| B | 0106321 | molecular_function | S-(hydroxymethyl)glutathione dehydrogenase (NADP+) activity |
| B | 0106322 | molecular_function | S-(hydroxymethyl)glutathione dehydrogenase (NAD+) activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A 400 |
| Chain | Residue |
| A | CYS99 |
| A | CYS102 |
| A | CYS105 |
| A | CYS113 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A 401 |
| Chain | Residue |
| A | CYS47 |
| A | HIS69 |
| A | GLU70 |
| A | CYS177 |
| site_id | AC3 |
| Number of Residues | 30 |
| Details | BINDING SITE FOR RESIDUE NAD A 402 |
| Chain | Residue |
| A | TYR95 |
| A | THR181 |
| A | GLY202 |
| A | GLY204 |
| A | THR205 |
| A | VAL206 |
| A | ASP226 |
| A | ILE227 |
| A | LYS231 |
| A | CYS271 |
| A | ILE272 |
| A | VAL277 |
| A | VAL295 |
| A | GLY296 |
| A | VAL297 |
| A | THR320 |
| A | ALA321 |
| A | PHE322 |
| A | ARG372 |
| A | HOH395 |
| A | HOH404 |
| A | HOH460 |
| A | HOH480 |
| A | HOH493 |
| A | HOH500 |
| A | HOH555 |
| A | HOH593 |
| A | HOH631 |
| A | HOH644 |
| A | HIS48 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1 |
| Chain | Residue |
| A | VAL316 |
| A | LYS318 |
| A | HOH452 |
| A | HOH505 |
| A | HOH578 |
| B | VAL316 |
| B | LYS318 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 380 |
| Chain | Residue |
| A | SER276 |
| A | ARG279 |
| A | HOH399 |
| A | HOH524 |
| site_id | AC6 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 381 |
| Chain | Residue |
| A | HIS249 |
| A | ASP250 |
| A | LYS251 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 382 |
| Chain | Residue |
| A | CYS102 |
| A | LYS103 |
| A | HOH442 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 A 383 |
| Chain | Residue |
| A | ARG307 |
| A | PHE309 |
| A | HOH396 |
| A | HOH470 |
| B | SER300 |
| B | GLY301 |
| site_id | AC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 384 |
| Chain | Residue |
| A | LYS287 |
| A | HOH583 |
| A | HOH648 |
| site_id | BC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 A 385 |
| Chain | Residue |
| A | ARG117 |
| B | LYS287 |
| B | PHE309 |
| B | THR313 |
| B | HOH386 |
| site_id | BC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B 400 |
| Chain | Residue |
| B | CYS99 |
| B | CYS102 |
| B | CYS105 |
| B | CYS113 |
| site_id | BC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B 401 |
| Chain | Residue |
| B | CYS47 |
| B | HIS69 |
| B | GLU70 |
| B | CYS177 |
| site_id | BC4 |
| Number of Residues | 31 |
| Details | BINDING SITE FOR RESIDUE NAD B 402 |
| Chain | Residue |
| B | HOH422 |
| B | HOH435 |
| B | HOH449 |
| B | HOH500 |
| B | HOH521 |
| B | HOH541 |
| B | HOH597 |
| B | HOH631 |
| B | HOH674 |
| B | HIS48 |
| B | TYR95 |
| B | CYS177 |
| B | THR181 |
| B | GLY202 |
| B | GLY204 |
| B | THR205 |
| B | VAL206 |
| B | ASP226 |
| B | ILE227 |
| B | LYS231 |
| B | CYS271 |
| B | ILE272 |
| B | VAL277 |
| B | VAL295 |
| B | GLY296 |
| B | VAL297 |
| B | THR320 |
| B | ALA321 |
| B | PHE322 |
| B | ARG372 |
| B | HOH385 |
| site_id | BC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 B 380 |
| Chain | Residue |
| B | HIS249 |
| B | ASP250 |
| B | LYS251 |
| site_id | BC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 B 381 |
| Chain | Residue |
| B | SER276 |
| B | ARG279 |
| B | SER305 |
| B | HOH423 |
| site_id | BC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 B 382 |
| Chain | Residue |
| B | TYR43 |
| B | PRO168 |
| B | LEU169 |
| B | ASP170 |
| B | HOH613 |
| site_id | BC8 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE SO4 B 383 |
| Chain | Residue |
| B | ASN197 |
| B | ARG221 |
| B | TRP333 |
| B | HOH468 |
| B | HOH469 |
| B | HOH534 |
| B | HOH590 |
| B | HOH647 |
| site_id | BC9 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 B 384 |
| Chain | Residue |
| B | CYS102 |
| B | LYS103 |
Functional Information from PROSITE/UniProt
| site_id | PS00059 |
| Number of Residues | 15 |
| Details | ADH_ZINC Zinc-containing alcohol dehydrogenases signature. GHEaAGIvesvGegV |
| Chain | Residue | Details |
| A | GLY68-VAL82 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 14 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:38308388, ECO:0000269|Ref.13, ECO:0007744|PDB:3UKO, ECO:0007744|PDB:4GL4, ECO:0007744|PDB:4JJI, ECO:0007744|PDB:4L0Q, ECO:0007744|PDB:8CO4 |
| Chain | Residue | Details |
| A | CYS47 | |
| B | CYS99 | |
| B | CYS102 | |
| B | CYS105 | |
| B | CYS113 | |
| B | CYS177 | |
| A | HIS69 | |
| A | CYS99 | |
| A | CYS102 | |
| A | CYS105 | |
| A | CYS113 | |
| A | CYS177 | |
| B | CYS47 | |
| B | HIS69 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 14 |
| Details | BINDING: BINDING => ECO:0000269|Ref.13, ECO:0007744|PDB:3UKO, ECO:0007744|PDB:4GL4, ECO:0007744|PDB:4JJI, ECO:0007744|PDB:4L0Q |
| Chain | Residue | Details |
| A | HIS48 | |
| B | GLY202 | |
| B | ASP226 | |
| B | ILE272 | |
| B | VAL295 | |
| B | THR320 | |
| A | GLU70 | |
| A | GLY202 | |
| A | ASP226 | |
| A | ILE272 | |
| A | VAL295 | |
| A | THR320 | |
| B | HIS48 | |
| B | GLU70 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P06525 |
| Chain | Residue | Details |
| A | THR49 | |
| B | THR49 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|Ref.13, ECO:0007744|PDB:3UKO, ECO:0007744|PDB:4JJI, ECO:0007744|PDB:4L0Q |
| Chain | Residue | Details |
| A | LYS231 | |
| A | ARG372 | |
| B | LYS231 | |
| B | ARG372 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | MOD_RES: N-acetylalanine => ECO:0007744|PubMed:22223895 |
| Chain | Residue | Details |
| A | ALA2 | |
| B | ALA2 |
