3THI

THIAMINASE I FROM BACILLUS THIAMINOLYTICUS

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005576	cellular_component	extracellular region
A	0009230	biological_process	thiamine catabolic process
A	0016740	molecular_function	transferase activity
A	0050332	molecular_function	thiamine pyridinylase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 380

Chain	Residue
A	HIS282
A	HIS282
A	PRO283
A	PRO283
A	GLU284
A	LEU285

---

site_id	NUC
Number of Residues	1
Details	CYS 113 IS THE ACTIVE SITE NUCLEOPHILE.

Chain	Residue
A	CYS113

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile

Chain	Residue	Details
A	CYS113

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton acceptor

Chain	Residue	Details
A	GLU241

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	3
Details	M-CSA 357

Chain	Residue	Details
A	CYS113	activator, covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
A	GLU241	activator, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	ASP272	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, steric role

---