3T2I

Tetragonal thermolysin in the presence of sarcosine

Functional Information from GO Data

Chain	GOid	namespace	contents
E	0004222	molecular_function	metalloendopeptidase activity
E	0006508	biological_process	proteolysis

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA E 317

Chain	Residue
E	ASP138
E	GLU177
E	ASP185
E	GLU187
E	GLU190
E	CA318
E	HOH414

---

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA E 318

Chain	Residue
E	ASP185
E	GLU190
E	CA317
E	HOH421
E	HOH457
E	GLU177
E	ASN183

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA E 319

Chain	Residue
E	ASP57
E	ASP59
E	GLN61
E	HOH447
E	HOH463
E	HOH467

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA E 320

Chain	Residue
E	TYR193
E	THR194
E	ILE197
E	ASP200
E	HOH422
E	HOH462

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN E 321

Chain	Residue
E	HIS142
E	HIS146
E	GLU166
E	CL324

---

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN E 322

Chain	Residue
E	HIS250
E	HOH401
E	HOH402
E	HOH403

---

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CL E 323

Chain	Residue
E	GLY12
E	LEU14
E	GLY15
E	ASP16
E	LYS18

---

site_id	AC8
Number of Residues	8
Details	BINDING SITE FOR RESIDUE CL E 324

Chain	Residue
E	HIS142
E	GLU143
E	HIS146
E	TYR157
E	GLU166
E	HIS231
E	ZN321
E	HOH499

---

site_id	AC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL E 325

Chain	Residue
E	ARG285
E	HOH517

---

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NA E 326

Chain	Residue
E	TYR211
E	HOH472
E	HOH503
E	HOH518
E	HOH519
E	HOH572

---

site_id	BC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SAR E 2001

Chain	Residue
E	ALA209
E	LYS210
E	TYR211
E	GLY212
E	HOH519

---

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SAR E 2002

Chain	Residue
E	ASN19
E	ASN21
E	ARG35
E	HOH419
E	HOH456

---

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SAR E 2003

Chain	Residue
E	TYR179
E	ALA180
E	ASN181
E	ARG269
E	HOH584

---

site_id	BC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE DMS E 2011

Chain	Residue
E	TYR66
E	HIS216
E	HOH543
E	HOH578

---

site_id	BC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE DMS E 2012

Chain	Residue
E	SER206
E	TYR242

---

Functional Information from PROSITE/UniProt

site_id	PS00142
Number of Residues	10
Details	ZINC_PROTEASE Neutral zinc metallopeptidases, zinc-binding region signature. VVAHELTHAV

Chain	Residue	Details
E	VAL139-VAL148

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU10095","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"4808703","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PROSITE-ProRule","id":"PRU10095","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"4808703","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	Binding site: {}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	7
Details	M-CSA 176

Chain

Residue

Details

---