Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3SQV

Crystal Structure of E. coli O157:H7 E3 ubiquitin ligase, NleL, with a human E2, UbcH7

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004842	molecular_function	ubiquitin-protein transferase activity
A	0016567	biological_process	protein ubiquitination
B	0004842	molecular_function	ubiquitin-protein transferase activity
B	0016567	biological_process	protein ubiquitination
C	0000151	cellular_component	ubiquitin ligase complex
C	0000209	biological_process	protein polyubiquitination
C	0003713	molecular_function	transcription coactivator activity
C	0003723	molecular_function	RNA binding
C	0004842	molecular_function	ubiquitin-protein transferase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006355	biological_process	regulation of DNA-templated transcription
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0008283	biological_process	cell population proliferation
C	0016567	biological_process	protein ubiquitination
C	0016740	molecular_function	transferase activity
C	0019787	molecular_function	ubiquitin-like protein transferase activity
C	0019899	molecular_function	enzyme binding
C	0031398	biological_process	positive regulation of protein ubiquitination
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0032446	biological_process	protein modification by small protein conjugation
C	0036211	biological_process	protein modification process
C	0044770	biological_process	cell cycle phase transition
C	0045893	biological_process	positive regulation of DNA-templated transcription
C	0051443	biological_process	positive regulation of ubiquitin-protein transferase activity
C	0061631	molecular_function	ubiquitin conjugating enzyme activity
C	0070979	biological_process	protein K11-linked ubiquitination
C	0071383	biological_process	cellular response to steroid hormone stimulus
C	0071385	biological_process	cellular response to glucocorticoid stimulus
C	0097027	molecular_function	ubiquitin-protein transferase activator activity
C	0098793	cellular_component	presynapse
C	1903955	biological_process	positive regulation of protein targeting to mitochondrion
D	0000151	cellular_component	ubiquitin ligase complex
D	0000209	biological_process	protein polyubiquitination
D	0003713	molecular_function	transcription coactivator activity
D	0003723	molecular_function	RNA binding
D	0004842	molecular_function	ubiquitin-protein transferase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006355	biological_process	regulation of DNA-templated transcription
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0008283	biological_process	cell population proliferation
D	0016567	biological_process	protein ubiquitination
D	0016740	molecular_function	transferase activity
D	0019787	molecular_function	ubiquitin-like protein transferase activity
D	0019899	molecular_function	enzyme binding
D	0031398	biological_process	positive regulation of protein ubiquitination
D	0031625	molecular_function	ubiquitin protein ligase binding
D	0032446	biological_process	protein modification by small protein conjugation
D	0036211	biological_process	protein modification process
D	0044770	biological_process	cell cycle phase transition
D	0045893	biological_process	positive regulation of DNA-templated transcription
D	0051443	biological_process	positive regulation of ubiquitin-protein transferase activity
D	0061631	molecular_function	ubiquitin conjugating enzyme activity
D	0070979	biological_process	protein K11-linked ubiquitination
D	0071383	biological_process	cellular response to steroid hormone stimulus
D	0071385	biological_process	cellular response to glucocorticoid stimulus
D	0097027	molecular_function	ubiquitin-protein transferase activator activity
D	0098793	cellular_component	presynapse
D	1903955	biological_process	positive regulation of protein targeting to mitochondrion

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 2

Chain	Residue
A	GLY486
A	MET487
A	GLN488
A	GLU489

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 4

Chain	Residue
A	HIS463
A	PRO464
A	GLY465

site_id	AC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 A 5

Chain	Residue
A	ASN217

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 6

Chain	Residue
A	TRP187
B	SER212
B	ASP232
A	ALA183

site_id	AC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 A 7

Chain	Residue
A	ASP322

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL A 783

Chain	Residue
A	LYS283
A	THR305
A	LYS324
B	LYS283

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 1

Chain	Residue
B	HIS463
B	PRO464
B	GLY465

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 3

Chain	Residue
B	GLY486
B	MET487
B	GLN488
B	GLU489

site_id	AC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 9

Chain	Residue
B	SER391
B	LEU392
B	ASP393

site_id	BC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE GOL C 155

Chain	Residue
C	GLY-1

Functional Information from PROSITE/UniProt

site_id	PS00183
Number of Residues	16
Details	UBC_1 Ubiquitin-conjugating (UBC) active site signature. YHPNIdek.GqVCLpvI

Chain	Residue	Details
C	TYR75-ILE90

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Glycyl thioester intermediate => ECO:0000255\|PROSITE-ProRule:PRU00388, ECO:0000255\|PROSITE-ProRule:PRU10133

Chain	Residue	Details
C	CYS86
D	CYS86

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0007744\|PubMed:19608861

Chain	Residue	Details
C	LYS131
D	LYS131

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	1
Details	M-CSA 438

Chain	Residue	Details
C	CYS86	nucleofuge

site_id	MCSA2
Number of Residues	1
Details	M-CSA 438

Chain	Residue	Details
D	CYS86	nucleofuge

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)