3S1S

Characterization and crystal structure of the type IIG restriction endonuclease BpuSI

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0003677	molecular_function	DNA binding
A	0008168	molecular_function	methyltransferase activity
A	0008170	molecular_function	N-methyltransferase activity
A	0009007	molecular_function	site-specific DNA-methyltransferase (adenine-specific) activity
A	0009307	biological_process	DNA restriction-modification system
A	0016740	molecular_function	transferase activity
A	0032259	biological_process	methylation
A	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	BINDING SITE FOR RESIDUE SAH A 900

Chain	Residue
A	HIS293
A	ILE356
A	PHE360
A	ASP388
A	VAL389
A	ASN406
A	PRO408
A	IOD938
A	HOH1111
A	HOH1235
A	GLU294
A	ALA329
A	ALA330
A	GLY331
A	SER332
A	ASN334
A	LEU335
A	ASP355

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO A 901

Chain	Residue
A	TRP589
A	ASN592
A	ASN593
A	HOH1306

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 903

Chain	Residue
A	GLU135
A	ILE165
A	ALA170
A	GLY171
A	THR172
A	HOH1313

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE EDO A 904

Chain	Residue
A	MSE347
A	ARG349
A	HOH1209

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO A 905

Chain	Residue
A	GLU216
A	VAL376
A	SER377
A	HOH1112

---

site_id	AC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE EDO A 906

Chain	Residue
A	SER82
A	ASP277
A	ASN334
A	ALA337
A	THR338
A	ARG367
A	EDO917
A	HOH1244

---

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 907

Chain	Residue
A	ARG228
A	PRO229
A	GLY230
A	GLU395
A	IOD953

---

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 908

Chain	Residue
A	ARG98
A	HIS122
A	PRO127
A	LYS703
A	HOH1294

---

site_id	AC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 909

Chain	Residue
A	PRO138
A	ILE139
A	VAL140
A	ARG272
A	HOH1082
A	HOH1116
A	HOH1190

---

site_id	BC1
Number of Residues	8
Details	BINDING SITE FOR RESIDUE EDO A 910

Chain	Residue
A	GLU204
A	ASP221
A	ALA222
A	ILE223
A	LEU263
A	SER264
A	HOH1001
A	HOH1221

---

site_id	BC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE EDO A 911

Chain	Residue
A	ARG317
A	HOH1210

---

site_id	BC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE EDO A 912

Chain	Residue
A	ARG88
A	HOH1151
A	HOH1199

---

site_id	BC4
Number of Residues	10
Details	BINDING SITE FOR RESIDUE EDO A 913

Chain	Residue
A	ASP240
A	ASN242
A	VAL243
A	SER365
A	ALA381
A	PRO382
A	ILE384
A	HOH1037
A	HOH1038
A	HOH1108

---

site_id	BC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE EDO A 914

Chain	Residue
A	ARG433
A	TRP631
A	HOH1284

---

site_id	BC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE EDO A 915

Chain	Residue
A	GLU30
A	HIS50

---

site_id	BC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE EDO A 916

Chain	Residue
A	GLY486
A	ASN487

---

site_id	BC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO A 917

Chain	Residue
A	TYR17
A	LYS305
A	EDO906
A	IOD968
A	HOH1270

---

site_id	BC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE EDO A 918

Chain	Residue
A	GLY439
A	ASN475

---

site_id	CC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE EDO A 919

Chain	Residue
A	SER619
A	GLY617
A	ALA618

---

site_id	CC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE EDO A 920

Chain	Residue
A	GLU234
A	ARG237
A	PHE245

---

site_id	CC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO A 921

Chain	Residue
A	LEU53
A	GLY55
A	MSE852
A	HOH1072

---

site_id	CC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MN A 930

Chain	Residue
A	GLU21
A	ASP60
A	GLU76
A	VAL77
A	HOH1129
A	HOH1218

---

site_id	CC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE IOD A 931

Chain	Residue
A	ARG736
A	GLU791

---

site_id	CC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE IOD A 932

Chain	Residue
A	ASP415

---

site_id	CC7
Number of Residues	1
Details	BINDING SITE FOR RESIDUE IOD A 933

Chain	Residue
A	PRO218

---

site_id	CC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE IOD A 934

Chain	Residue
A	HIS107
A	HIS122
A	ASP694

---

site_id	CC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE IOD A 935

Chain	Residue
A	SER176
A	ASN179
A	ASN261

---

site_id	DC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE IOD A 936

Chain	Residue
A	GLU795
A	SER798

---

site_id	DC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE IOD A 937

Chain	Residue
A	GLU247
A	GLU249

---

site_id	DC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE IOD A 938

Chain	Residue
A	SAH900

---

site_id	DC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE IOD A 939

Chain	Residue
A	HOH1106

---

site_id	DC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE IOD A 940

Chain	Residue
A	LYS699

---

site_id	DC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE IOD A 941

Chain	Residue
A	TYR173

---

site_id	DC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE IOD A 942

Chain	Residue
A	VAL148
A	ASN344

---

site_id	DC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE IOD A 944

Chain	Residue
A	LYS541
A	ARG542

---

site_id	DC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE IOD A 945

Chain	Residue
A	LYS64
A	LYS103

---

site_id	EC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE IOD A 947

Chain	Residue
A	HOH1250

---

site_id	EC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE IOD A 948

Chain	Residue
A	ARG88

---

site_id	EC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE IOD A 949

Chain	Residue
A	ARG237
A	SER246

---

site_id	EC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE IOD A 950

Chain	Residue
A	GLU726

---

site_id	EC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE IOD A 951

Chain	Residue
A	ASP677

---

site_id	EC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE IOD A 952

Chain	Residue
A	HOH1248

---

site_id	EC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE IOD A 953

Chain	Residue
A	ARG228
A	ARG231
A	EDO907

---

site_id	EC8
Number of Residues	1
Details	BINDING SITE FOR RESIDUE IOD A 954

Chain	Residue
A	HIS826

---

site_id	EC9
Number of Residues	1
Details	BINDING SITE FOR RESIDUE IOD A 955

Chain	Residue
A	GLN374

---

site_id	FC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE IOD A 956

Chain	Residue
A	LYS796

---

site_id	FC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE IOD A 957

Chain	Residue
A	ARG666

---

site_id	FC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE IOD A 958

Chain	Residue
A	HIS424
A	GLN428

---

site_id	FC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE IOD A 959

Chain	Residue
A	ARG640
A	HOH1176

---

site_id	FC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE IOD A 960

Chain	Residue
A	VAL650

---

site_id	FC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE IOD A 961

Chain	Residue
A	TYR692
A	LYS707

---

site_id	FC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE IOD A 962

Chain	Residue
A	SER176
A	THR177

---

site_id	FC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE IOD A 963

Chain	Residue
A	GLU504
A	VAL505

---

site_id	FC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE IOD A 964

Chain	Residue
A	ASN539
A	ASN539

---

site_id	GC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE IOD A 967

Chain	Residue
A	SER12
A	SER545

---

site_id	GC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE IOD A 968

Chain	Residue
A	TYR17
A	SER82
A	EDO917

---

Functional Information from PROSITE/UniProt

site_id	PS00092
Number of Residues	7
Details	N6_MTASE N-6 Adenine-specific DNA methylases signature. VVMNPPY

Chain	Residue	Details
A	VAL403-TYR409

---