3RZP
Crystal Structure of the C194A mutant of 7-cyano-7-deazaguanine reductase, QueF from Vibrio cholerae complexed with preQ1
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006400 | biological_process | tRNA modification |
| A | 0008616 | biological_process | tRNA queuosine(34) biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0033739 | molecular_function | preQ1 synthase activity |
| A | 0046857 | molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006400 | biological_process | tRNA modification |
| B | 0008616 | biological_process | tRNA queuosine(34) biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0033739 | molecular_function | preQ1 synthase activity |
| B | 0046857 | molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006400 | biological_process | tRNA modification |
| C | 0008616 | biological_process | tRNA queuosine(34) biosynthetic process |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0033739 | molecular_function | preQ1 synthase activity |
| C | 0046857 | molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006400 | biological_process | tRNA modification |
| D | 0008616 | biological_process | tRNA queuosine(34) biosynthetic process |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0033739 | molecular_function | preQ1 synthase activity |
| D | 0046857 | molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE PRF A 290 |
| Chain | Residue |
| A | TRP62 |
| A | ILE93 |
| A | GLU94 |
| A | SER95 |
| A | ASP201 |
| A | PHE232 |
| A | HIS233 |
| A | GLU234 |
| A | ILE267 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL A 291 |
| Chain | Residue |
| A | ARG262 |
| A | GLY263 |
| B | GLY263 |
| B | HOH344 |
| site_id | AC3 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE PRF B 290 |
| Chain | Residue |
| B | TRP62 |
| B | LEU92 |
| B | ILE93 |
| B | GLU94 |
| B | SER95 |
| B | ASP201 |
| B | PHE232 |
| B | HIS233 |
| B | GLU234 |
| B | ILE267 |
| site_id | AC4 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE PRF C 290 |
| Chain | Residue |
| C | TRP62 |
| C | LEU92 |
| C | ILE93 |
| C | GLU94 |
| C | SER95 |
| C | ASP201 |
| C | PHE232 |
| C | HIS233 |
| C | GLU234 |
| C | ILE267 |
| C | HOH293 |
| site_id | AC5 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE PRF D 290 |
| Chain | Residue |
| D | TRP62 |
| D | ILE93 |
| D | GLU94 |
| D | SER95 |
| D | ASP201 |
| D | PHE232 |
| D | HIS233 |
| D | GLU234 |
| D | ILE267 |
| D | HOH320 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL D 291 |
| Chain | Residue |
| C | GLN199 |
| C | ARG262 |
| C | GLY263 |
| D | ARG262 |
| D | GLY263 |
| D | HOH315 |
| D | HOH331 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Thioimide intermediate","evidences":[{"source":"HAMAP-Rule","id":"MF_00817","evidenceCode":"ECO:0000255"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"JAN-2013","submissionDatabase":"PDB data bank","title":"Crystal structures of 7-cyano-7-deazaguanine reductase, QueF, from Vibrio cholerae.","authoringGroup":["Center for structural genomics of infectious diseases (CSGID)"]}}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Proton donor","evidences":[{"source":"HAMAP-Rule","id":"MF_00817","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 20 |
| Details | Binding site: {} |
| Chain | Residue | Details |
