3RQS
Crystal Structure of human L-3- Hydroxyacyl-CoA dehydrogenase (EC1.1.1.35) from mitochondria at the resolution 2.0 A, Northeast Structural Genomics Consortium Target HR487, Mitochondrial Protein Partnership
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0003857 | molecular_function | 3-hydroxyacyl-CoA dehydrogenase activity |
A | 0005654 | cellular_component | nucleoplasm |
A | 0005737 | cellular_component | cytoplasm |
A | 0005739 | cellular_component | mitochondrion |
A | 0005759 | cellular_component | mitochondrial matrix |
A | 0006631 | biological_process | fatty acid metabolic process |
A | 0006635 | biological_process | fatty acid beta-oxidation |
A | 0009410 | biological_process | response to xenobiotic stimulus |
A | 0009725 | biological_process | response to hormone |
A | 0014823 | biological_process | response to activity |
A | 0016491 | molecular_function | oxidoreductase activity |
A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
A | 0016740 | molecular_function | transferase activity |
A | 0032868 | biological_process | response to insulin |
A | 0042802 | molecular_function | identical protein binding |
A | 0046676 | biological_process | negative regulation of insulin secretion |
A | 0050796 | biological_process | regulation of insulin secretion |
A | 0070403 | molecular_function | NAD+ binding |
A | 0120162 | biological_process | positive regulation of cold-induced thermogenesis |
B | 0003857 | molecular_function | 3-hydroxyacyl-CoA dehydrogenase activity |
B | 0005654 | cellular_component | nucleoplasm |
B | 0005737 | cellular_component | cytoplasm |
B | 0005739 | cellular_component | mitochondrion |
B | 0005759 | cellular_component | mitochondrial matrix |
B | 0006631 | biological_process | fatty acid metabolic process |
B | 0006635 | biological_process | fatty acid beta-oxidation |
B | 0009410 | biological_process | response to xenobiotic stimulus |
B | 0009725 | biological_process | response to hormone |
B | 0014823 | biological_process | response to activity |
B | 0016491 | molecular_function | oxidoreductase activity |
B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
B | 0016740 | molecular_function | transferase activity |
B | 0032868 | biological_process | response to insulin |
B | 0042802 | molecular_function | identical protein binding |
B | 0046676 | biological_process | negative regulation of insulin secretion |
B | 0050796 | biological_process | regulation of insulin secretion |
B | 0070403 | molecular_function | NAD+ binding |
B | 0120162 | biological_process | positive regulation of cold-induced thermogenesis |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE GOL A 315 |
Chain | Residue |
A | ASN220 |
A | VAL224 |
A | VAL265 |
A | THR269 |
A | THR270 |
A | ILE273 |
A | GOL316 |
A | HOH373 |
site_id | AC2 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE GOL A 316 |
Chain | Residue |
A | TYR264 |
A | GOL315 |
A | GOL317 |
A | HOH371 |
B | GLY251 |
A | ASN173 |
site_id | AC3 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE GOL A 317 |
Chain | Residue |
A | MSE178 |
A | PRO255 |
A | MSE256 |
A | LEU261 |
A | GOL316 |
A | HOH385 |
B | ALA252 |
B | HOH423 |
site_id | AC4 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE GOL A 318 |
Chain | Residue |
A | PRO86 |
A | LYS87 |
A | GLU140 |
A | ARG165 |
A | LYS192 |
A | HOH382 |
site_id | AC5 |
Number of Residues | 10 |
Details | BINDING SITE FOR RESIDUE GOL B 315 |
Chain | Residue |
B | ASN220 |
B | VAL224 |
B | VAL265 |
B | THR269 |
B | THR270 |
B | ILE273 |
B | GOL317 |
B | HOH368 |
B | HOH531 |
B | HOH552 |
site_id | AC6 |
Number of Residues | 9 |
Details | BINDING SITE FOR RESIDUE GOL B 316 |
Chain | Residue |
B | MSE38 |
B | ALA119 |
B | ILE120 |
B | GLU122 |
B | ASN147 |
B | HOH332 |
B | HOH354 |
B | HOH449 |
B | HOH524 |
site_id | AC7 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE GOL B 317 |
Chain | Residue |
A | GLY251 |
A | HOH394 |
B | PHE172 |
B | ASN173 |
B | LEU223 |
B | VAL265 |
B | GOL315 |
B | HOH356 |
Functional Information from PROSITE/UniProt
site_id | PS00067 |
Number of Residues | 25 |
Details | 3HCDH 3-hydroxyacyl-CoA dehydrogenase signature. DtpGFIvNRllvPYLmeair.LYerG |
Chain | Residue | Details |
A | ASP213-GLY237 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 14 |
Details | BINDING: BINDING => ECO:0000269|PubMed:10231530, ECO:0000269|PubMed:10840044 |
Chain | Residue | Details |
A | GLY34 | |
B | GLU122 | |
B | LYS127 | |
B | SER149 | |
B | ASN173 | |
B | LYS305 | |
A | ASP57 | |
A | GLU122 | |
A | LYS127 | |
A | SER149 | |
A | ASN173 | |
A | LYS305 | |
B | GLY34 | |
B | ASP57 |
site_id | SWS_FT_FI2 |
Number of Residues | 4 |
Details | BINDING: BINDING => ECO:0000269|PubMed:10840044 |
Chain | Residue | Details |
A | SER73 | |
A | LYS80 | |
B | SER73 | |
B | LYS80 |
site_id | SWS_FT_FI3 |
Number of Residues | 2 |
Details | SITE: Important for catalytic activity => ECO:0000305 |
Chain | Residue | Details |
A | HIS170 | |
B | HIS170 |
site_id | SWS_FT_FI4 |
Number of Residues | 4 |
Details | MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:Q61425 |
Chain | Residue | Details |
A | LYS80 | |
A | LYS206 | |
B | LYS80 | |
B | LYS206 |
site_id | SWS_FT_FI5 |
Number of Residues | 18 |
Details | MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:Q61425 |
Chain | Residue | Details |
A | LYS81 | |
B | LYS81 | |
B | LYS87 | |
B | LYS136 | |
B | LYS185 | |
B | LYS192 | |
B | LYS202 | |
B | LYS212 | |
B | LYS241 | |
B | LYS312 | |
A | LYS87 | |
A | LYS136 | |
A | LYS185 | |
A | LYS192 | |
A | LYS202 | |
A | LYS212 | |
A | LYS241 | |
A | LYS312 |
site_id | SWS_FT_FI6 |
Number of Residues | 4 |
Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q61425 |
Chain | Residue | Details |
A | LYS125 | |
A | LYS179 | |
B | LYS125 | |
B | LYS179 |
site_id | SWS_FT_FI7 |
Number of Residues | 2 |
Details | MOD_RES: N6-(2-hydroxyisobutyryl)lysine => ECO:0000269|PubMed:29192674 |
Chain | Residue | Details |
A | LYS127 | |
B | LYS127 |
Catalytic Information from CSA
site_id | MCSA1 |
Number of Residues | 4 |
Details | M-CSA 336 |
Chain | Residue | Details |
A | SER149 | electrostatic stabiliser, hydrogen bond donor |
A | HIS170 | proton acceptor, proton donor |
A | GLU182 | electrostatic stabiliser, hydrogen bond acceptor, increase basicity |
A | ASN220 | electrostatic stabiliser, hydrogen bond donor |
site_id | MCSA2 |
Number of Residues | 4 |
Details | M-CSA 336 |
Chain | Residue | Details |
B | SER149 | electrostatic stabiliser, hydrogen bond donor |
B | HIS170 | proton acceptor, proton donor |
B | GLU182 | electrostatic stabiliser, hydrogen bond acceptor, increase basicity |
B | ASN220 | electrostatic stabiliser, hydrogen bond donor |