3RKU

Substrate Fingerprint and the Structure of NADP+ Dependent Serine Dehydrogenase from Saccharomyces cerevisiae complexed with NADP+

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004090	molecular_function	carbonyl reductase (NADPH) activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0031132	molecular_function	serine 3-dehydrogenase activity
A	0045149	biological_process	acetoin metabolic process
A	0052588	molecular_function	diacetyl reductase ((S)-acetoin forming) (NAD+) activity
B	0004090	molecular_function	carbonyl reductase (NADPH) activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0031132	molecular_function	serine 3-dehydrogenase activity
B	0045149	biological_process	acetoin metabolic process
B	0052588	molecular_function	diacetyl reductase ((S)-acetoin forming) (NAD+) activity
C	0004090	molecular_function	carbonyl reductase (NADPH) activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0031132	molecular_function	serine 3-dehydrogenase activity
C	0045149	biological_process	acetoin metabolic process
C	0052588	molecular_function	diacetyl reductase ((S)-acetoin forming) (NAD+) activity
D	0004090	molecular_function	carbonyl reductase (NADPH) activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0031132	molecular_function	serine 3-dehydrogenase activity
D	0045149	biological_process	acetoin metabolic process
D	0052588	molecular_function	diacetyl reductase ((S)-acetoin forming) (NAD+) activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	34
Details	BINDING SITE FOR RESIDUE NAP A 268

Chain	Residue
A	GLY20
A	ASP75
A	ILE76
A	ASN102
A	ALA103
A	GLY104
A	LYS105
A	GLY154
A	SER155
A	TYR168
A	LYS172
A	SER22
A	PRO198
A	GLY199
A	VAL201
A	THR203
A	GLU204
A	PHE205
A	HOH274
A	HOH296
A	HOH297
A	HOH303
A	ALA23
A	HOH330
A	HOH331
A	HOH348
A	HOH551
A	HOH555
A	GLY24
A	ILE25
A	ALA47
A	ARG48
A	ARG49
A	LEU74

---

site_id	AC2
Number of Residues	34
Details	BINDING SITE FOR RESIDUE NAP B 268

Chain	Residue
B	GLY20
B	SER22
B	ALA23
B	GLY24
B	ILE25
B	ALA47
B	ARG48
B	ARG49
B	LEU74
B	ASP75
B	ILE76
B	ASN102
B	ALA103
B	GLY104
B	LYS105
B	LEU153
B	GLY154
B	SER155
B	TYR168
B	LYS172
B	PRO198
B	GLY199
B	VAL201
B	THR203
B	GLU204
B	PHE205
B	HOH275
B	HOH277
B	HOH290
B	HOH291
B	HOH319
B	HOH334
B	HOH417
B	HOH554

---

site_id	AC3
Number of Residues	33
Details	BINDING SITE FOR RESIDUE NAP C 268

Chain	Residue
C	HOH419
C	HOH544
C	GLY20
C	SER22
C	ALA23
C	GLY24
C	ILE25
C	ALA47
C	ARG48
C	ARG49
C	LEU74
C	ASP75
C	ILE76
C	ASN102
C	ALA103
C	GLY104
C	LYS105
C	LEU153
C	GLY154
C	TYR168
C	LYS172
C	PRO198
C	GLY199
C	VAL201
C	THR203
C	GLU204
C	PHE205
C	HOH269
C	HOH274
C	HOH279
C	HOH284
C	HOH296
C	HOH351

---

site_id	AC4
Number of Residues	34
Details	BINDING SITE FOR RESIDUE NAP D 268

Chain	Residue
D	GLY20
D	SER22
D	ALA23
D	GLY24
D	ILE25
D	ALA47
D	ARG48
D	ARG49
D	LEU74
D	ASP75
D	ILE76
D	ASN102
D	ALA103
D	GLY104
D	LYS105
D	LEU153
D	GLY154
D	TYR168
D	LYS172
D	PRO198
D	GLY199
D	VAL201
D	THR203
D	GLU204
D	PHE205
D	HOH269
D	HOH271
D	HOH273
D	HOH283
D	HOH285
D	HOH297
D	HOH336
D	HOH375
D	HOH553

---

Functional Information from PROSITE/UniProt

site_id	PS00061
Number of Residues	29
Details	ADH_SHORT Short-chain dehydrogenases/reductases family signature. SiagrdayptGsiYCASKFAVgAFTdSLR

Chain	Residue	Details
A	SER155-ARG183

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"Proton donor","evidences":[{"source":"UniProtKB","id":"O93868","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Lowers pKa of active site Tyr","evidences":[{"source":"UniProtKB","id":"O93868","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"L0E2Z4","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"APR-2011","submissionDatabase":"PDB data bank","title":"Substrate fingerprint and the structure of NADP(+)-dependent serine dehydrogenase from Saccharomyces cerevisiae complexed with NADP(+).","authors":["Huether R.","Pacheco C.M.","Duax W.L."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"O93868","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"19779198","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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