3RIS
Crystal structure of the catalytic domain of UCHL5, a proteasome-associated human deubiquitinating enzyme, reveals an unproductive form of the enzyme
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004843 | molecular_function | cysteine-type deubiquitinase activity |
| A | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| A | 0016579 | biological_process | protein deubiquitination |
| A | 0031011 | cellular_component | Ino80 complex |
| A | 0070628 | molecular_function | proteasome binding |
| B | 0004843 | molecular_function | cysteine-type deubiquitinase activity |
| B | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| B | 0016579 | biological_process | protein deubiquitination |
| B | 0031011 | cellular_component | Ino80 complex |
| B | 0070628 | molecular_function | proteasome binding |
| C | 0004843 | molecular_function | cysteine-type deubiquitinase activity |
| C | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| C | 0016579 | biological_process | protein deubiquitination |
| C | 0031011 | cellular_component | Ino80 complex |
| C | 0070628 | molecular_function | proteasome binding |
| D | 0004843 | molecular_function | cysteine-type deubiquitinase activity |
| D | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| D | 0016579 | biological_process | protein deubiquitination |
| D | 0031011 | cellular_component | Ino80 complex |
| D | 0070628 | molecular_function | proteasome binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 A 241 |
| Chain | Residue |
| A | ARG137 |
| A | GLN138 |
| A | HOH252 |
| A | HOH291 |
| C | GLN146 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 B 241 |
| Chain | Residue |
| D | GLN146 |
| B | ARG137 |
| B | GLN138 |
| B | HOH248 |
| B | HOH269 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE GOL B 242 |
| Chain | Residue |
| B | ARG174 |
| B | GLY189 |
| B | ALA190 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 D 241 |
| Chain | Residue |
| B | GLN146 |
| D | ARG137 |
| D | GLN138 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Nucleophile => ECO:0000305 |
| Chain | Residue | Details |
| A | CYS88 | |
| B | CYS88 | |
| C | CYS88 | |
| D | CYS88 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton donor => ECO:0000250 |
| Chain | Residue | Details |
| A | HIS164 | |
| B | HIS164 | |
| C | HIS164 | |
| D | HIS164 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | SITE: Important for enzyme activity => ECO:0000250 |
| Chain | Residue | Details |
| A | ASP179 | |
| B | ASP179 | |
| C | ASP179 | |
| D | ASP179 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:Q9WUP7 |
| Chain | Residue | Details |
| A | LYS47 | |
| B | LYS47 | |
| C | LYS47 | |
| D | LYS47 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| A | LYS158 | |
| B | LYS158 | |
| C | LYS158 | |
| D | LYS158 |
