3RHL
Crystal structure of the E673A/C707A double mutant of the C-Terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 38 |
| Details | BINDING SITE FOR RESIDUE NAP A 903 |
| Chain | Residue |
| A | VAL570 |
| A | GLY630 |
| A | GLY634 |
| A | GLN635 |
| A | PHE648 |
| A | THR649 |
| A | GLY650 |
| A | SER651 |
| A | VAL654 |
| A | HIS657 |
| A | ILE658 |
| A | ILE571 |
| A | ALA673 |
| A | LEU674 |
| A | GLY675 |
| A | ALA707 |
| A | GLU804 |
| A | PHE806 |
| A | PHE872 |
| A | HOH1056 |
| A | HOH1080 |
| A | HOH1089 |
| A | PRO572 |
| A | HOH1100 |
| A | HOH1119 |
| A | HOH1170 |
| A | HOH1193 |
| A | HOH1206 |
| A | HOH1244 |
| A | HOH1260 |
| A | HOH1284 |
| A | HOH1349 |
| A | TRP573 |
| A | ASN574 |
| A | LYS597 |
| A | ALA599 |
| A | GLN600 |
| A | SER629 |
| site_id | AC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 A 3001 |
| Chain | Residue |
| A | LYS852 |
| C | ARG554 |
| site_id | AC3 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 A 3006 |
| Chain | Residue |
| A | ARG636 |
| A | HOH1216 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 A 3011 |
| Chain | Residue |
| A | ARG764 |
| A | TYR799 |
| site_id | AC5 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE SO4 A 3014 |
| Chain | Residue |
| A | GLY881 |
| A | LYS882 |
| A | HOH1023 |
| A | HOH1175 |
| A | HOH1270 |
| B | LYS669 |
| B | LEU893 |
| B | ARG894 |
| B | ILE895 |
| B | HOH1089 |
| site_id | AC6 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE SO4 A 3015 |
| Chain | Residue |
| A | LYS669 |
| A | LEU893 |
| A | ARG894 |
| A | ILE895 |
| A | HOH1233 |
| B | GLY881 |
| B | LYS882 |
| B | HOH1041 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 3018 |
| Chain | Residue |
| A | ARG551 |
| A | ARG554 |
| A | HOH1224 |
| C | LYS852 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 3021 |
| Chain | Residue |
| A | ASN548 |
| A | HOH1091 |
| A | HOH1342 |
| B | GLN528 |
| site_id | AC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 3024 |
| Chain | Residue |
| A | ASN738 |
| A | GLU741 |
| A | ARG781 |
| A | HOH1354 |
| site_id | BC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 2000 |
| Chain | Residue |
| A | TYR575 |
| A | MET578 |
| A | ASN706 |
| A | THR866 |
| A | HOH1359 |
| site_id | BC2 |
| Number of Residues | 38 |
| Details | BINDING SITE FOR RESIDUE NAP B 903 |
| Chain | Residue |
| B | LEU674 |
| B | GLY675 |
| B | ALA707 |
| B | GLU804 |
| B | PHE806 |
| B | PHE872 |
| B | HOH1111 |
| B | HOH1124 |
| B | HOH1180 |
| B | HOH1216 |
| B | HOH1245 |
| B | HOH1263 |
| B | HOH1264 |
| B | HOH1278 |
| B | HOH1293 |
| B | HOH1308 |
| B | HOH1320 |
| B | HOH1347 |
| B | VAL570 |
| B | ILE571 |
| B | PRO572 |
| B | TRP573 |
| B | ASN574 |
| B | LYS597 |
| B | ALA599 |
| B | GLN600 |
| B | SER629 |
| B | GLY630 |
| B | GLY634 |
| B | GLN635 |
| B | PHE648 |
| B | THR649 |
| B | GLY650 |
| B | SER651 |
| B | VAL654 |
| B | HIS657 |
| B | ILE658 |
| B | ALA673 |
| site_id | BC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 B 3003 |
| Chain | Residue |
| B | ARG554 |
| D | ASP851 |
| D | LYS852 |
| site_id | BC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 B 3004 |
| Chain | Residue |
| B | LYS852 |
| B | HOH1036 |
| D | ARG554 |
| site_id | BC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 B 3005 |
| Chain | Residue |
| B | ARG636 |
| B | PRO641 |
| B | HOH1078 |
| B | HOH1322 |
| site_id | BC6 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE SO4 B 3009 |
| Chain | Residue |
| B | ARG764 |
| site_id | BC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 B 3019 |
| Chain | Residue |
| B | ARG551 |
| B | ARG554 |
| D | TYR848 |
| site_id | BC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 B 3022 |
| Chain | Residue |
| A | GLN528 |
| B | ASN548 |
| B | HOH1102 |
| B | HOH1274 |
| site_id | BC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 B 3025 |
| Chain | Residue |
| B | ASN738 |
| B | GLU741 |
| B | ARG781 |
| B | HOH1028 |
| site_id | CC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL B 2001 |
| Chain | Residue |
| B | TYR575 |
| B | ASN706 |
| B | THR866 |
| B | HOH1351 |
| site_id | CC2 |
| Number of Residues | 36 |
| Details | BINDING SITE FOR RESIDUE NAP C 903 |
| Chain | Residue |
| C | VAL570 |
| C | ILE571 |
| C | PRO572 |
| C | TRP573 |
| C | ASN574 |
| C | LYS597 |
| C | ALA599 |
| C | GLN600 |
| C | SER629 |
| C | GLY630 |
| C | GLY634 |
| C | GLN635 |
| C | PHE648 |
| C | THR649 |
| C | GLY650 |
| C | SER651 |
| C | VAL654 |
| C | HIS657 |
| C | ILE658 |
| C | ALA673 |
| C | LEU674 |
| C | GLY675 |
| C | ALA707 |
| C | GLU804 |
| C | PHE806 |
| C | PHE872 |
| C | HOH1061 |
| C | HOH1117 |
| C | HOH1173 |
| C | HOH1228 |
| C | HOH1252 |
| C | HOH1255 |
| C | HOH1256 |
| C | HOH1259 |
| C | HOH1262 |
| C | HOH1352 |
| site_id | CC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 C 3002 |
| Chain | Residue |
| A | ARG554 |
| C | LYS852 |
| C | HOH1321 |
| site_id | CC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 C 3007 |
| Chain | Residue |
| C | ARG636 |
| C | PRO641 |
| site_id | CC5 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE SO4 C 3010 |
| Chain | Residue |
| C | ARG764 |
| site_id | CC6 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE SO4 C 3013 |
| Chain | Residue |
| C | LYS669 |
| C | LEU893 |
| C | ARG894 |
| C | ILE895 |
| C | HOH1190 |
| C | HOH1201 |
| D | GLY881 |
| D | LYS882 |
| D | HOH1050 |
| site_id | CC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 C 3017 |
| Chain | Residue |
| A | TYR848 |
| C | ARG551 |
| C | ARG554 |
| C | HOH1112 |
| site_id | CC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 C 3023 |
| Chain | Residue |
| C | ASN548 |
| C | HOH1191 |
| C | HOH1223 |
| D | GLN528 |
| site_id | CC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 C 3026 |
| Chain | Residue |
| A | HOH1277 |
| C | ASN738 |
| C | ARG781 |
| C | HOH1085 |
| site_id | DC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 C 3028 |
| Chain | Residue |
| A | LEU416 |
| A | THR417 |
| C | LYS415 |
| C | ARG742 |
| C | HOH1102 |
| site_id | DC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL C 2002 |
| Chain | Residue |
| C | TYR575 |
| C | ASN706 |
| C | THR866 |
| C | HOH1363 |
| site_id | DC3 |
| Number of Residues | 36 |
| Details | BINDING SITE FOR RESIDUE NAP D 903 |
| Chain | Residue |
| D | VAL570 |
| D | ILE571 |
| D | PRO572 |
| D | TRP573 |
| D | ASN574 |
| D | LYS597 |
| D | ALA599 |
| D | GLN600 |
| D | SER629 |
| D | GLY630 |
| D | GLY634 |
| D | GLN635 |
| D | PHE648 |
| D | THR649 |
| D | GLY650 |
| D | SER651 |
| D | VAL654 |
| D | HIS657 |
| D | ILE658 |
| D | ALA673 |
| D | LEU674 |
| D | GLY675 |
| D | ALA707 |
| D | GLU804 |
| D | PHE806 |
| D | PHE872 |
| D | HOH1054 |
| D | HOH1151 |
| D | HOH1178 |
| D | HOH1200 |
| D | HOH1231 |
| D | HOH1258 |
| D | HOH1260 |
| D | HOH1263 |
| D | HOH1334 |
| D | HOH1355 |
| site_id | DC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 D 3008 |
| Chain | Residue |
| D | ARG636 |
| D | PRO641 |
| D | HOH1246 |
| site_id | DC5 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE SO4 D 3012 |
| Chain | Residue |
| D | ARG764 |
| site_id | DC6 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE SO4 D 3016 |
| Chain | Residue |
| C | GLY881 |
| C | LYS882 |
| C | HOH1016 |
| D | LYS669 |
| D | LEU893 |
| D | ARG894 |
| D | ILE895 |
| D | HOH1053 |
| D | HOH1158 |
| D | HOH1184 |
| D | HOH1318 |
| site_id | DC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 D 3020 |
| Chain | Residue |
| B | TYR848 |
| D | ARG551 |
| D | ARG554 |
| site_id | DC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 D 3027 |
| Chain | Residue |
| D | THR445 |
| D | ASN738 |
| D | ARG781 |
| D | HOH1104 |
| site_id | DC9 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE GOL D 2003 |
| Chain | Residue |
| D | ASN706 |
| D | THR866 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton acceptor => ECO:0000305|PubMed:17302434 |
| Chain | Residue | Details |
| A | ALA673 | |
| B | ALA673 | |
| C | ALA673 | |
| D | ALA673 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton donor => ECO:0000305|PubMed:17302434, ECO:0000305|PubMed:21540484, ECO:0007744|PDB:2O2Q, ECO:0007744|PDB:3RHO |
| Chain | Residue | Details |
| A | ALA707 | |
| B | ALA707 | |
| C | ALA707 | |
| D | ALA707 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 16 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:17302434, ECO:0000269|PubMed:21540484, ECO:0007744|PDB:2O2Q, ECO:0007744|PDB:2O2R, ECO:0007744|PDB:3RHJ, ECO:0007744|PDB:3RHL, ECO:0007744|PDB:3RHO, ECO:0007744|PDB:3RHQ, ECO:0007744|PDB:3RHR |
| Chain | Residue | Details |
| A | ILE571 | |
| C | LYS597 | |
| C | GLY630 | |
| C | GLY650 | |
| D | ILE571 | |
| D | LYS597 | |
| D | GLY630 | |
| D | GLY650 | |
| A | LYS597 | |
| A | GLY630 | |
| A | GLY650 | |
| B | ILE571 | |
| B | LYS597 | |
| B | GLY630 | |
| B | GLY650 | |
| C | ILE571 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:17302434, ECO:0000269|PubMed:21540484, ECO:0007744|PDB:2O2Q, ECO:0007744|PDB:3RHJ, ECO:0007744|PDB:3RHL, ECO:0007744|PDB:3RHO, ECO:0007744|PDB:3RHQ, ECO:0007744|PDB:3RHR |
| Chain | Residue | Details |
| A | ALA673 | |
| A | GLU804 | |
| B | ALA673 | |
| B | GLU804 | |
| C | ALA673 | |
| C | GLU804 | |
| D | ALA673 | |
| D | GLU804 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:21540484, ECO:0007744|PDB:3RHO |
| Chain | Residue | Details |
| A | LYS757 | |
| B | LYS757 | |
| C | LYS757 | |
| D | LYS757 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 12 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:O75891 |
| Chain | Residue | Details |
| A | SER629 | |
| D | SER629 | |
| D | SER631 | |
| D | SER825 | |
| A | SER631 | |
| A | SER825 | |
| B | SER629 | |
| B | SER631 | |
| B | SER825 | |
| C | SER629 | |
| C | SER631 | |
| C | SER825 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:Q8K009 |
| Chain | Residue | Details |
| A | LYS660 | |
| B | LYS660 | |
| C | LYS660 | |
| D | LYS660 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:Q8R0Y6 |
| Chain | Residue | Details |
| A | LYS767 | |
| B | LYS767 | |
| C | LYS767 | |
| D | LYS767 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 4 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q3SY69 |
| Chain | Residue | Details |
| A | LYS882 | |
| B | LYS882 | |
| C | LYS882 | |
| D | LYS882 |
Catalytic Information from CSA
