3RGW
Crystal structure at 1.5 A resolution of an H2-reduced, O2-tolerant hydrogenase from Ralstonia eutropha unmasks a novel iron-sulfur cluster
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| L | 0005515 | molecular_function | protein binding |
| L | 0005886 | cellular_component | plasma membrane |
| L | 0008901 | molecular_function | ferredoxin hydrogenase activity |
| L | 0016020 | cellular_component | membrane |
| L | 0016151 | molecular_function | nickel cation binding |
| L | 0016491 | molecular_function | oxidoreductase activity |
| L | 0033748 | molecular_function | hydrogenase (acceptor) activity |
| L | 0046872 | molecular_function | metal ion binding |
| S | 0005515 | molecular_function | protein binding |
| S | 0005886 | cellular_component | plasma membrane |
| S | 0008901 | molecular_function | ferredoxin hydrogenase activity |
| S | 0009055 | molecular_function | electron transfer activity |
| S | 0009061 | biological_process | anaerobic respiration |
| S | 0009375 | cellular_component | ferredoxin hydrogenase complex |
| S | 0016020 | cellular_component | membrane |
| S | 0016491 | molecular_function | oxidoreductase activity |
| S | 0033748 | molecular_function | hydrogenase (acceptor) activity |
| S | 0044569 | cellular_component | [Ni-Fe] hydrogenase complex |
| S | 0046872 | molecular_function | metal ion binding |
| S | 0051536 | molecular_function | iron-sulfur cluster binding |
| S | 0051538 | molecular_function | 3 iron, 4 sulfur cluster binding |
| S | 0051539 | molecular_function | 4 iron, 4 sulfur cluster binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE NFU L 1004 |
| Chain | Residue |
| L | CYS75 |
| L | PRO552 |
| L | THR553 |
| L | CYS597 |
| L | CYS600 |
| L | CYS78 |
| L | CYS81 |
| L | HIS82 |
| L | ALA528 |
| L | PRO529 |
| L | ARG530 |
| L | LEU533 |
| L | VAL551 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG L 1005 |
| Chain | Residue |
| L | GLU56 |
| L | CYS549 |
| L | HIS603 |
| L | HOH668 |
| L | HOH726 |
| L | HOH877 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SF4 S 1001 |
| Chain | Residue |
| S | HIS187 |
| S | CYS190 |
| S | ARG193 |
| S | CYS215 |
| S | LEU216 |
| S | CYS221 |
| S | ILE243 |
| site_id | AC4 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE F3S S 1002 |
| Chain | Residue |
| L | LYS226 |
| S | THR226 |
| S | ASN228 |
| S | CYS230 |
| S | TRP235 |
| S | PRO242 |
| S | CYS249 |
| S | ILE250 |
| S | CYS252 |
| site_id | AC5 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE F4S S 1003 |
| Chain | Residue |
| S | CYS17 |
| S | THR18 |
| S | CYS19 |
| S | CYS20 |
| S | SER114 |
| S | CYS115 |
| S | CYS120 |
| S | CYS149 |
Functional Information from PROSITE/UniProt
| site_id | PS00507 |
| Number of Residues | 26 |
| Details | NI_HGENASE_L_1 Nickel-dependent hydrogenases large subunit signature 1. RGLEvilkgrdprdawafvERiCGVC |
| Chain | Residue | Details |
| L | ARG53-CYS78 |
| site_id | PS00508 |
| Number of Residues | 10 |
| Details | NI_HGENASE_L_2 Nickel-dependent hydrogenases large subunit signature 2. FDPCLACst.H |
| Chain | Residue | Details |
| L | PHE594-HIS603 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 11 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P21853","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
