3R3E
The glutathione bound structure of YqjG, a glutathione transferase homolog from Escherichia coli K-12
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004364 | molecular_function | glutathione transferase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016672 | molecular_function | oxidoreductase activity, acting on a sulfur group of donors, quinone or similar compound as acceptor |
| A | 0042803 | molecular_function | protein homodimerization activity |
| B | 0004364 | molecular_function | glutathione transferase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016672 | molecular_function | oxidoreductase activity, acting on a sulfur group of donors, quinone or similar compound as acceptor |
| B | 0042803 | molecular_function | protein homodimerization activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE GSH A 400 |
| Chain | Residue |
| A | CYS63 |
| A | SO4330 |
| A | HOH464 |
| A | TRP65 |
| A | TRP96 |
| A | ARG130 |
| A | THR132 |
| A | VAL133 |
| A | PRO134 |
| A | GLU148 |
| A | SER149 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 329 |
| Chain | Residue |
| A | ARG73 |
| A | LYS74 |
| A | HOH471 |
| site_id | AC3 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE SO4 A 330 |
| Chain | Residue |
| A | TRP65 |
| A | ARG68 |
| A | GLU148 |
| A | ALA150 |
| A | TYR187 |
| A | ARG241 |
| A | GSH400 |
| A | HOH406 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 A 331 |
| Chain | Residue |
| A | THR35 |
| A | ALA36 |
| A | THR158 |
| A | ALA159 |
| A | HOH347 |
| A | HOH383 |
| A | HOH442 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 332 |
| Chain | Residue |
| A | LYS261 |
| A | HIS262 |
| A | HOH342 |
| A | HOH468 |
| site_id | AC6 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 333 |
| Chain | Residue |
| A | HIS262 |
| A | ASP266 |
| A | TYR267 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 334 |
| Chain | Residue |
| A | GLY168 |
| A | ASP169 |
| A | GLN234 |
| B | PRO80 |
| site_id | AC8 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE GSH B 400 |
| Chain | Residue |
| B | CYS63 |
| B | TRP65 |
| B | TRP96 |
| B | ARG130 |
| B | VAL131 |
| B | THR132 |
| B | VAL133 |
| B | PRO134 |
| B | ASN147 |
| B | GLU148 |
| B | SER149 |
| B | HOH415 |
| site_id | AC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 B 329 |
| Chain | Residue |
| B | LYS261 |
| B | HIS262 |
| B | HOH378 |
| site_id | BC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 B 330 |
| Chain | Residue |
| B | TRP65 |
| B | ARG68 |
| B | ALA150 |
| B | TYR187 |
| B | ARG241 |
| B | HOH415 |
| B | HOH423 |
| site_id | BC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 B 331 |
| Chain | Residue |
| B | ARG73 |
| B | HOH434 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Nucleophile => ECO:0000269|PubMed:22955277 |
| Chain | Residue | Details |
| A | CYS63 | |
| B | CYS63 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor/acceptor => ECO:0000305|PubMed:22955277 |
| Chain | Residue | Details |
| A | TYR195 | |
| B | TYR195 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:22955277, ECO:0000269|Ref.5 |
| Chain | Residue | Details |
| A | TRP96 | |
| A | ARG130 | |
| A | GLU148 | |
| B | TRP96 | |
| B | ARG130 | |
| B | GLU148 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | SITE: Lowers pKa of active site Cys => ECO:0000305 |
| Chain | Residue | Details |
| A | TYR253 | |
| A | TYR296 | |
| B | TYR253 | |
| B | TYR296 |
