3QPG

Crystal Structures of Escherichia coli Aspartate Aminotransferase Reconstituted with 1-Deaza-Pyridoxal 5'-Phosphate: Internal Aldimine and Stable L-Aspartate External Aldimine

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004069	molecular_function	L-aspartate:2-oxoglutarate aminotransferase activity
A	0004838	molecular_function	L-tyrosine-2-oxoglutarate transaminase activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006520	biological_process	amino acid metabolic process
A	0008483	molecular_function	transaminase activity
A	0009058	biological_process	biosynthetic process
A	0009094	biological_process	L-phenylalanine biosynthetic process
A	0016740	molecular_function	transferase activity
A	0030170	molecular_function	pyridoxal phosphate binding
A	0033585	biological_process	L-phenylalanine biosynthetic process from chorismate via phenylpyruvate
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 409

Chain	Residue
A	ARG327
A	GLN328
A	HOH699
A	HOH720
A	HOH732

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 410

Chain	Residue
A	LYS133
A	ARG134
A	ARG372
A	ALA406

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 411

Chain	Residue
A	LYS146
A	ASN150
A	GLU155
A	VAL156
A	ARG157
A	HOH726

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 412

Chain	Residue
A	ALA127
A	VAL132
A	GLY153
A	LEU154
A	HOH568

---

site_id	AC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 413

Chain	Residue
A	ARG348
A	ASP349

---

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 414

Chain	Residue
A	LYS49
A	THR55
A	THR74
A	ASN76
A	HOH662

---

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 415

Chain	Residue
A	PRO84
A	ARG88
A	HOH577
A	HOH690

---

site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 416

Chain	Residue
A	GLY232
A	LEU233
A	GLU234
A	GLU235
A	HOH473
A	HOH618
A	HOH723

---

site_id	AC9
Number of Residues	9
Details	BINDING SITE FOR RESIDUE EDO A 417

Chain	Residue
A	PRO84
A	GLY87
A	ARG88
A	GLN91
A	ARG108
A	THR109
A	HOH489
A	HOH495
A	HOH692

---

site_id	BC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO A 418

Chain	Residue
A	HOH6
A	THR90
A	LEU94
A	GLN227
A	SER254
A	TYR256
A	ASN259

---

site_id	BC2
Number of Residues	12
Details	BINDING SITE FOR RESIDUE EDO A 419

Chain	Residue
A	PRO196
A	THR197
A	GLY198
A	ARG334
A	GLN356
A	ASN357
A	GLY358
A	MET359
A	PHE360
A	SER361
A	PHE362
A	HOH485

---

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO A 420

Chain	Residue
A	HIS247
A	LYS248
A	GLU249
A	LYS367

---

site_id	BC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL A 421

Chain	Residue
A	ARG108
A	HOH670

---

site_id	BC5
Number of Residues	17
Details	BINDING SITE FOR RESIDUE 3QP A 422

Chain	Residue
A	HOH11
A	ILE25
A	GLY46
A	TYR77
A	GLY114
A	GLY115
A	THR116
A	TRP142
A	ASN195
A	ASP223
A	TYR226
A	SER255
A	SER257
A	LYS258
A	ARG266
A	ARG292
A	ARG386

---

Functional Information from PROSITE/UniProt

site_id	PS00105
Number of Residues	14
Details	AA_TRANSFER_CLASS_1 Aminotransferases class-I pyridoxal-phosphate attachment site. SYSKnfGLyNERVG

Chain	Residue	Details
A	SER255-GLY268

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PDB","id":"1ART","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1CZE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QPG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4DBC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PDB","id":"1AIB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ART","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1CZE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1SPA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QPG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4DBC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PDB","id":"1AIB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ART","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1CZE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1SPA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QPG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4DBC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PDB","id":"1AHG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1AIB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ARG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ART","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1CZE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3QPG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4DBC","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Modified residue: {"description":"N6-(pyridoxal phosphate)lysine","evidences":[{"source":"PubMed","id":"11148029","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"1993208","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"3298240","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9891001","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1AAW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1AHE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1AHF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1AHX","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1AHY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ARI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ARS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASB","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASF","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ASN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1B4X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1CZC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1CZE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1G4V","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1G4X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1G7W","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1G7X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1IX6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1IX7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1IX8","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1QIR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1QIS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1QIT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1YOO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2D5Y","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2D61","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2D63","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2D7Y","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3AAT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3ZZJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5EAA","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	3
Details	M-CSA 777

Chain	Residue	Details
A	TRP142	steric role
A	ASP223	proton shuttle (general acid/base)
A	LYS258	proton shuttle (general acid/base)

---