3QIM
Histidine 416 of the periplamsic binding protein NikA is essential for nickel uptake in Escherichia coli
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005515 | molecular_function | protein binding |
| A | 0015675 | biological_process | nickel cation transport |
| A | 0015833 | biological_process | peptide transport |
| A | 0016020 | cellular_component | membrane |
| A | 0016151 | molecular_function | nickel cation binding |
| A | 0020037 | molecular_function | heme binding |
| A | 0030288 | cellular_component | outer membrane-bounded periplasmic space |
| A | 0042597 | cellular_component | periplasmic space |
| A | 0043190 | cellular_component | ATP-binding cassette (ABC) transporter complex |
| A | 0046914 | molecular_function | transition metal ion binding |
| A | 0050919 | biological_process | negative chemotaxis |
| A | 0051540 | molecular_function | metal cluster binding |
| A | 0055085 | biological_process | transmembrane transport |
| A | 0098716 | biological_process | nickel cation import across plasma membrane |
| A | 1904680 | molecular_function | peptide transmembrane transporter activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0015675 | biological_process | nickel cation transport |
| B | 0015833 | biological_process | peptide transport |
| B | 0016020 | cellular_component | membrane |
| B | 0016151 | molecular_function | nickel cation binding |
| B | 0020037 | molecular_function | heme binding |
| B | 0030288 | cellular_component | outer membrane-bounded periplasmic space |
| B | 0042597 | cellular_component | periplasmic space |
| B | 0043190 | cellular_component | ATP-binding cassette (ABC) transporter complex |
| B | 0046914 | molecular_function | transition metal ion binding |
| B | 0050919 | biological_process | negative chemotaxis |
| B | 0051540 | molecular_function | metal cluster binding |
| B | 0055085 | biological_process | transmembrane transport |
| B | 0098716 | biological_process | nickel cation import across plasma membrane |
| B | 1904680 | molecular_function | peptide transmembrane transporter activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 503 |
| Chain | Residue |
| A | PRO159 |
| A | ILE166 |
| A | LEU167 |
| A | HOH529 |
| B | HOH624 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 504 |
| Chain | Residue |
| A | TYR310 |
| A | VAL273 |
| A | ASN274 |
| A | LYS275 |
| A | GLN309 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL A 505 |
| Chain | Residue |
| A | PHE147 |
| A | LYS148 |
| A | ASN149 |
| A | LYS157 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL A 506 |
| Chain | Residue |
| A | PRO48 |
| A | LYS52 |
| A | SER53 |
| A | TRP54 |
| site_id | AC5 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE ACT A 507 |
| Chain | Residue |
| A | ARG457 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 508 |
| Chain | Residue |
| A | GLU221 |
| A | SER353 |
| A | PHE394 |
| A | ARG396 |
| A | SO4518 |
| site_id | AC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE ACT A 509 |
| Chain | Residue |
| A | ASN482 |
| A | GLN496 |
| site_id | AC8 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL A 510 |
| Chain | Residue |
| A | GLU86 |
| A | ARG89 |
| A | PRO144 |
| A | PHE147 |
| A | HIS150 |
| A | GLN366 |
| A | HOH757 |
| A | HOH771 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ACT A 511 |
| Chain | Residue |
| A | ASN261 |
| A | GLU262 |
| A | LEU263 |
| A | HOH620 |
| A | HOH629 |
| site_id | BC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ACT A 512 |
| Chain | Residue |
| A | ASN235 |
| A | ALA237 |
| A | GLN423 |
| A | HOH647 |
| site_id | BC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE ACT A 513 |
| Chain | Residue |
| A | ALA486 |
| A | HOH540 |
| A | HOH689 |
| site_id | BC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE ACT A 514 |
| Chain | Residue |
| A | ARG89 |
| A | ARG95 |
| A | VAL108 |
| A | ASP109 |
| A | VAL110 |
| A | ASN281 |
| A | HOH616 |
| site_id | BC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL A 515 |
| Chain | Residue |
| A | THR23 |
| A | ARG97 |
| A | HOH666 |
| A | HOH737 |
| B | GLN309 |
| B | LYS314 |
| site_id | BC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ACT A 516 |
| Chain | Residue |
| A | GLY219 |
| A | ASN220 |
| A | GLY222 |
| A | LEU223 |
| site_id | BC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 A 517 |
| Chain | Residue |
| A | THR23 |
| A | PRO24 |
| A | GLN26 |
| A | HOH581 |
| A | HOH591 |
| site_id | BC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 A 518 |
| Chain | Residue |
| A | GLN385 |
| A | PHE394 |
| A | HIS395 |
| A | ARG396 |
| A | GOL508 |
| A | HOH592 |
| site_id | BC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 A 519 |
| Chain | Residue |
| A | LYS330 |
| A | ASP331 |
| A | ILE332 |
| A | ARG365 |
| B | LYS148 |
| site_id | BC9 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL B 503 |
| Chain | Residue |
| B | TRP10 |
| B | PRO11 |
| B | GLY219 |
| B | ASN220 |
| B | GLY222 |
| B | LEU223 |
| site_id | CC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE ACT B 504 |
| Chain | Residue |
| A | LYS335 |
| A | HOH775 |
| B | ARG341 |
| site_id | CC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ACT B 505 |
| Chain | Residue |
| B | TYR22 |
| B | ARG97 |
| B | TRP100 |
| B | ACT506 |
| B | HOH580 |
| site_id | CC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE ACT B 506 |
| Chain | Residue |
| B | HOH620 |
| B | TYR22 |
| B | TRP100 |
| B | ARG137 |
| B | ACT505 |
| B | ACT507 |
| B | HOH580 |
| site_id | CC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ACT B 507 |
| Chain | Residue |
| B | TRP398 |
| B | ACT506 |
| B | HOH580 |
| B | HOH582 |
| site_id | CC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ACT B 508 |
| Chain | Residue |
| B | GLN385 |
| B | HIS395 |
| B | ARG396 |
| B | HOH554 |
| site_id | CC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ACT B 509 |
| Chain | Residue |
| B | THR23 |
| B | GLN26 |
| B | GLU378 |
| B | HOH698 |
| site_id | CC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 B 510 |
| Chain | Residue |
| A | GLU343 |
| A | ARG384 |
| A | ARG389 |
| B | LEU430 |
| B | ARG457 |
| B | HOH595 |
Functional Information from PROSITE/UniProt
| site_id | PS01040 |
| Number of Residues | 23 |
| Details | SBP_BACTERIAL_5 Bacterial extracellular solute-binding proteins, family 5 signature. AkswthseDgkTWtFtLRDDVKF |
| Chain | Residue | Details |
| A | ALA51-PHE73 |
