3QFA
Crystal structure of the human thioredoxin reductase-thioredoxin complex
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0004791 | molecular_function | thioredoxin-disulfide reductase (NADPH) activity |
A | 0016491 | molecular_function | oxidoreductase activity |
A | 0016668 | molecular_function | oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor |
A | 0045454 | biological_process | cell redox homeostasis |
A | 0050660 | molecular_function | flavin adenine dinucleotide binding |
B | 0004791 | molecular_function | thioredoxin-disulfide reductase (NADPH) activity |
B | 0016491 | molecular_function | oxidoreductase activity |
B | 0016668 | molecular_function | oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor |
B | 0045454 | biological_process | cell redox homeostasis |
B | 0050660 | molecular_function | flavin adenine dinucleotide binding |
C | 0000122 | biological_process | negative regulation of transcription by RNA polymerase II |
C | 0003723 | molecular_function | RNA binding |
C | 0004791 | molecular_function | thioredoxin-disulfide reductase (NADPH) activity |
C | 0005515 | molecular_function | protein binding |
C | 0005576 | cellular_component | extracellular region |
C | 0005634 | cellular_component | nucleus |
C | 0005654 | cellular_component | nucleoplasm |
C | 0005737 | cellular_component | cytoplasm |
C | 0005829 | cellular_component | cytosol |
C | 0009314 | biological_process | response to radiation |
C | 0015035 | molecular_function | protein-disulfide reductase activity |
C | 0033138 | biological_process | positive regulation of peptidyl-serine phosphorylation |
C | 0042803 | molecular_function | protein homodimerization activity |
C | 0043388 | biological_process | positive regulation of DNA binding |
C | 0045454 | biological_process | cell redox homeostasis |
C | 0046826 | biological_process | negative regulation of protein export from nucleus |
C | 0047134 | molecular_function | protein-disulfide reductase (NAD(P)H) activity |
C | 0051897 | biological_process | positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction |
C | 0055114 | biological_process | obsolete oxidation-reduction process |
C | 0061692 | biological_process | cellular detoxification of hydrogen peroxide |
C | 0070062 | cellular_component | extracellular exosome |
C | 0071731 | biological_process | response to nitric oxide |
C | 2000170 | biological_process | positive regulation of peptidyl-cysteine S-nitrosylation |
D | 0000122 | biological_process | negative regulation of transcription by RNA polymerase II |
D | 0003723 | molecular_function | RNA binding |
D | 0004791 | molecular_function | thioredoxin-disulfide reductase (NADPH) activity |
D | 0005515 | molecular_function | protein binding |
D | 0005576 | cellular_component | extracellular region |
D | 0005634 | cellular_component | nucleus |
D | 0005654 | cellular_component | nucleoplasm |
D | 0005737 | cellular_component | cytoplasm |
D | 0005829 | cellular_component | cytosol |
D | 0009314 | biological_process | response to radiation |
D | 0015035 | molecular_function | protein-disulfide reductase activity |
D | 0033138 | biological_process | positive regulation of peptidyl-serine phosphorylation |
D | 0042803 | molecular_function | protein homodimerization activity |
D | 0043388 | biological_process | positive regulation of DNA binding |
D | 0045454 | biological_process | cell redox homeostasis |
D | 0046826 | biological_process | negative regulation of protein export from nucleus |
D | 0047134 | molecular_function | protein-disulfide reductase (NAD(P)H) activity |
D | 0051897 | biological_process | positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction |
D | 0055114 | biological_process | obsolete oxidation-reduction process |
D | 0061692 | biological_process | cellular detoxification of hydrogen peroxide |
D | 0070062 | cellular_component | extracellular exosome |
D | 0071731 | biological_process | response to nitric oxide |
D | 2000170 | biological_process | positive regulation of peptidyl-cysteine S-nitrosylation |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 38 |
Details | BINDING SITE FOR RESIDUE FAD A 600 |
Chain | Residue |
A | ILE18 |
A | CYS59 |
A | VAL62 |
A | GLY63 |
A | CYS64 |
A | LYS67 |
A | ALA130 |
A | TYR131 |
A | GLY132 |
A | ALA160 |
A | THR161 |
A | GLY19 |
A | GLY162 |
A | SER180 |
A | TYR200 |
A | VAL201 |
A | ARG293 |
A | ILE300 |
A | GLY333 |
A | ASP334 |
A | GLU341 |
A | LEU342 |
A | GLY21 |
A | THR343 |
A | PRO344 |
A | HOH504 |
A | HOH524 |
A | HOH549 |
A | HOH572 |
A | HOH585 |
A | HOH591 |
B | HIS472 |
A | SER22 |
A | GLY23 |
A | LEU41 |
A | ASP42 |
A | PHE43 |
A | THR58 |
site_id | AC2 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE GOL A 801 |
Chain | Residue |
A | ASP231 |
A | LYS235 |
A | TYR405 |
A | TYR422 |
site_id | AC3 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE GOL A 802 |
Chain | Residue |
A | GLU368 |
A | ASN369 |
A | GLY384 |
A | LEU385 |
A | HOH545 |
A | HOH690 |
site_id | AC4 |
Number of Residues | 7 |
Details | BINDING SITE FOR RESIDUE GOL A 804 |
Chain | Residue |
A | GLN258 |
A | ASN428 |
A | LYS430 |
A | ASP431 |
A | ARG434 |
A | HOH578 |
A | HOH598 |
site_id | AC5 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE GOL A 803 |
Chain | Residue |
A | GLU387 |
A | ILE399 |
A | LYS486 |
A | ARG487 |
site_id | AC6 |
Number of Residues | 38 |
Details | BINDING SITE FOR RESIDUE FAD B 600 |
Chain | Residue |
A | HIS472 |
B | ILE18 |
B | GLY19 |
B | GLY21 |
B | SER22 |
B | GLY23 |
B | LEU41 |
B | ASP42 |
B | PHE43 |
B | GLY57 |
B | THR58 |
B | CYS59 |
B | VAL62 |
B | GLY63 |
B | CYS64 |
B | LYS67 |
B | TYR131 |
B | GLY132 |
B | ALA160 |
B | THR161 |
B | GLY162 |
B | TYR200 |
B | VAL201 |
B | ARG293 |
B | ILE300 |
B | GLY333 |
B | ASP334 |
B | GLU341 |
B | LEU342 |
B | THR343 |
B | PRO344 |
B | HOH509 |
B | HOH520 |
B | HOH521 |
B | HOH536 |
B | HOH542 |
B | HOH591 |
B | HOH602 |
site_id | AC7 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE GOL B 804 |
Chain | Residue |
B | ASN432 |
B | ARG434 |
B | LYS430 |
B | ASP431 |
site_id | AC8 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE GOL D 803 |
Chain | Residue |
D | ASP26 |
D | SER28 |
D | ALA29 |
D | THR30 |
D | SER35 |
D | LYS36 |
D | LYS39 |
D | GLU56 |
Functional Information from PROSITE/UniProt
site_id | PS00076 |
Number of Residues | 11 |
Details | PYRIDINE_REDOX_1 Pyridine nucleotide-disulphide oxidoreductases class-I active site. GGtCVnvGCIP |
Chain | Residue | Details |
A | GLY56-PRO66 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 4 |
Details | ACT_SITE: Nucleophile => ECO:0000269|PubMed:9108029 |
Chain | Residue | Details |
C | CYS32 | |
A | LEU465 | |
A | VAL484 | |
B | GLY172 | |
B | LYS192 | |
B | PHE208 | |
B | GLY213 | |
B | GLU281 | |
B | ILE316 | |
B | GLN348 | |
B | PRO371 | |
C | SER35 | |
B | GLY442 | |
B | LEU465 | |
B | VAL484 | |
D | CYS32 | |
D | SER35 | |
A | GLU281 | |
A | ILE316 | |
A | GLN348 | |
A | PRO371 | |
A | GLY442 |
site_id | SWS_FT_FI2 |
Number of Residues | 2 |
Details | SITE: Deprotonates C-terminal active site Cys => ECO:0000305 |
Chain | Residue | Details |
C | ASP26 | |
D | ASP26 | |
B | GLU311 | |
B | SER491 |
site_id | SWS_FT_FI3 |
Number of Residues | 4 |
Details | SITE: Contributes to redox potential value => ECO:0000305 |
Chain | Residue | Details |
C | GLY33 | |
C | PRO34 | |
D | GLY33 | |
D | PRO34 |
site_id | SWS_FT_FI4 |
Number of Residues | 4 |
Details | MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861 |
Chain | Residue | Details |
C | LYS3 | |
C | LYS39 | |
D | LYS3 | |
D | LYS39 |
site_id | SWS_FT_FI5 |
Number of Residues | 2 |
Details | MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:P10639 |
Chain | Residue | Details |
C | LYS8 | |
D | LYS8 |
site_id | SWS_FT_FI6 |
Number of Residues | 4 |
Details | MOD_RES: S-nitrosocysteine => ECO:0000269|PubMed:17260951 |
Chain | Residue | Details |
C | CYS62 | |
C | CYS69 | |
D | CYS62 | |
D | CYS69 |
site_id | SWS_FT_FI7 |
Number of Residues | 2 |
Details | MOD_RES: S-nitrosocysteine; alternate => ECO:0000269|PubMed:16408020, ECO:0000269|PubMed:17606900 |
Chain | Residue | Details |
C | SER73 | |
D | SER73 |
site_id | SWS_FT_FI8 |
Number of Residues | 2 |
Details | MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P10639 |
Chain | Residue | Details |
C | LYS94 | |
D | LYS94 |