3Q8E
Crystal structure of Protective Antigen W346F (pH 8.5)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005515 | molecular_function | protein binding |
| A | 0005576 | cellular_component | extracellular region |
| A | 0016020 | cellular_component | membrane |
| A | 0020002 | cellular_component | host cell plasma membrane |
| A | 0033644 | cellular_component | host cell membrane |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0043409 | biological_process | negative regulation of MAPK cascade |
| A | 0044164 | cellular_component | host cell cytosol |
| A | 0044174 | cellular_component | host cell endosome |
| A | 0044175 | cellular_component | host cell endosome membrane |
| A | 0044533 | biological_process | positive regulation of apoptotic process in another organism |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0051260 | biological_process | protein homooligomerization |
| A | 0090729 | molecular_function | toxin activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 736 |
| Chain | Residue |
| A | ASP179 |
| A | ASP181 |
| A | GLU188 |
| A | SER222 |
| A | LYS225 |
| A | ASP235 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 737 |
| Chain | Residue |
| A | ILE183 |
| A | GLU188 |
| A | HOH744 |
| A | ASP177 |
| A | ASP179 |
| A | ASP181 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 20 |
| Details | TRANSMEM: Beta stranded => ECO:0000269|PubMed:25778700, ECO:0000269|PubMed:32047164, ECO:0007744|PDB:3J9C, ECO:0007744|PDB:6PSN, ECO:0007744|PDB:6UZB, ECO:0007744|PDB:6UZD, ECO:0007744|PDB:6UZE |
| Chain | Residue | Details |
| A | GLU302-PHE313 | |
| A | ILE316-SER325 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 3 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15243628, ECO:0000269|PubMed:15326297, ECO:0000269|PubMed:21037566, ECO:0000269|PubMed:25778700, ECO:0000269|PubMed:32047164, ECO:0000269|PubMed:9039918, ECO:0007744|PDB:1ACC, ECO:0007744|PDB:1T6B, ECO:0007744|PDB:1TZN, ECO:0007744|PDB:1TZO, ECO:0007744|PDB:3J9C, ECO:0007744|PDB:3KWV, ECO:0007744|PDB:6PSN, ECO:0007744|PDB:6UZB, ECO:0007744|PDB:6UZD, ECO:0007744|PDB:6UZE |
| Chain | Residue | Details |
| A | ASP177 | |
| A | ASP181 | |
| A | ILE183 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15243628, ECO:0000269|PubMed:15326297, ECO:0000269|PubMed:21037566, ECO:0000269|PubMed:25778700, ECO:0000269|PubMed:32047164, ECO:0000269|PubMed:9039918, ECO:0007744|PDB:1ACC, ECO:0007744|PDB:1T6B, ECO:0007744|PDB:1TZO, ECO:0007744|PDB:3J9C, ECO:0007744|PDB:3KWV, ECO:0007744|PDB:6PSN, ECO:0007744|PDB:6UZB, ECO:0007744|PDB:6UZD, ECO:0007744|PDB:6UZE |
| Chain | Residue | Details |
| A | ASP179 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15326297, ECO:0000269|PubMed:21037566, ECO:0000269|PubMed:25778700, ECO:0000269|PubMed:32047164, ECO:0007744|PDB:1ACC, ECO:0007744|PDB:1TZN, ECO:0007744|PDB:1TZO, ECO:0007744|PDB:3J9C, ECO:0007744|PDB:3KWV, ECO:0007744|PDB:6PSN, ECO:0007744|PDB:6UZB, ECO:0007744|PDB:6UZD, ECO:0007744|PDB:6UZE |
| Chain | Residue | Details |
| A | GLU188 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15243628, ECO:0000269|PubMed:15326297, ECO:0000269|PubMed:21037566, ECO:0000269|PubMed:25778700, ECO:0000269|PubMed:32047164, ECO:0007744|PDB:1ACC, ECO:0007744|PDB:1T6B, ECO:0007744|PDB:1TZN, ECO:0007744|PDB:1TZO, ECO:0007744|PDB:3J9C, ECO:0007744|PDB:3KWV, ECO:0007744|PDB:6PSN, ECO:0007744|PDB:6UZB, ECO:0007744|PDB:6UZD, ECO:0007744|PDB:6UZE |
| Chain | Residue | Details |
| A | SER222 | |
| A | LYS225 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15243628, ECO:0000269|PubMed:15326297, ECO:0000269|PubMed:21037566, ECO:0000269|PubMed:32047164, ECO:0007744|PDB:1ACC, ECO:0007744|PDB:1T6B, ECO:0007744|PDB:1TZN, ECO:0007744|PDB:1TZO, ECO:0007744|PDB:3KWV, ECO:0007744|PDB:6PSN, ECO:0007744|PDB:6UZB, ECO:0007744|PDB:6UZD, ECO:0007744|PDB:6UZE |
| Chain | Residue | Details |
| A | ASP235 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | SITE: Cleavage; by FURIN => ECO:0000269|PubMed:11207581, ECO:0000269|PubMed:1438214, ECO:0000269|PubMed:1644824 |
| Chain | Residue | Details |
| A | ARG167 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | SITE: Alpha-clamp => ECO:0000269|PubMed:21037566 |
| Chain | Residue | Details |
| A | ARG178 | |
| A | LEU187 | |
| A | PHE236 | |
| A | PHE464 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | SITE: Cleavage; by chymotrypsin; required for translocation of LF and EF => ECO:0000269|PubMed:7961869 |
| Chain | Residue | Details |
| A | PHE314 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | SITE: Phi-clamp => ECO:0000269|PubMed:16051798, ECO:0000269|PubMed:25778700 |
| Chain | Residue | Details |
| A | PHE427 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | SITE: Essential for binding to cell receptor => ECO:0000269|PubMed:12771151 |
| Chain | Residue | Details |
| A | ASP683 |
