Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3PQD

Crystal structure of L-lactate dehydrogenase from Bacillus subtilis complexed with FBP and NAD+

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004459	molecular_function	L-lactate dehydrogenase activity
A	0005737	cellular_component	cytoplasm
A	0006089	biological_process	lactate metabolic process
A	0006090	biological_process	pyruvate metabolic process
A	0006096	biological_process	glycolytic process
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0019752	biological_process	carboxylic acid metabolic process
B	0003824	molecular_function	catalytic activity
B	0004459	molecular_function	L-lactate dehydrogenase activity
B	0005737	cellular_component	cytoplasm
B	0006089	biological_process	lactate metabolic process
B	0006090	biological_process	pyruvate metabolic process
B	0006096	biological_process	glycolytic process
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0019752	biological_process	carboxylic acid metabolic process
C	0003824	molecular_function	catalytic activity
C	0004459	molecular_function	L-lactate dehydrogenase activity
C	0005737	cellular_component	cytoplasm
C	0006089	biological_process	lactate metabolic process
C	0006090	biological_process	pyruvate metabolic process
C	0006096	biological_process	glycolytic process
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0019752	biological_process	carboxylic acid metabolic process
D	0003824	molecular_function	catalytic activity
D	0004459	molecular_function	L-lactate dehydrogenase activity
D	0005737	cellular_component	cytoplasm
D	0006089	biological_process	lactate metabolic process
D	0006090	biological_process	pyruvate metabolic process
D	0006096	biological_process	glycolytic process
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0019752	biological_process	carboxylic acid metabolic process
E	0003824	molecular_function	catalytic activity
E	0004459	molecular_function	L-lactate dehydrogenase activity
E	0005737	cellular_component	cytoplasm
E	0006089	biological_process	lactate metabolic process
E	0006090	biological_process	pyruvate metabolic process
E	0006096	biological_process	glycolytic process
E	0016491	molecular_function	oxidoreductase activity
E	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
E	0019752	biological_process	carboxylic acid metabolic process
F	0003824	molecular_function	catalytic activity
F	0004459	molecular_function	L-lactate dehydrogenase activity
F	0005737	cellular_component	cytoplasm
F	0006089	biological_process	lactate metabolic process
F	0006090	biological_process	pyruvate metabolic process
F	0006096	biological_process	glycolytic process
F	0016491	molecular_function	oxidoreductase activity
F	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
F	0019752	biological_process	carboxylic acid metabolic process
G	0003824	molecular_function	catalytic activity
G	0004459	molecular_function	L-lactate dehydrogenase activity
G	0005737	cellular_component	cytoplasm
G	0006089	biological_process	lactate metabolic process
G	0006090	biological_process	pyruvate metabolic process
G	0006096	biological_process	glycolytic process
G	0016491	molecular_function	oxidoreductase activity
G	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
G	0019752	biological_process	carboxylic acid metabolic process
H	0003824	molecular_function	catalytic activity
H	0004459	molecular_function	L-lactate dehydrogenase activity
H	0005737	cellular_component	cytoplasm
H	0006089	biological_process	lactate metabolic process
H	0006090	biological_process	pyruvate metabolic process
H	0006096	biological_process	glycolytic process
H	0016491	molecular_function	oxidoreductase activity
H	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
H	0019752	biological_process	carboxylic acid metabolic process

Functional Information from PROSITE/UniProt

site_id	PS00064
Number of Residues	7
Details	L_LDH L-lactate dehydrogenase active site. IGEHGDT

Chain	Residue	Details
A	ILE175-THR181

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Proton acceptor => ECO:0000255\|HAMAP-Rule:MF_00488, ECO:0000305\|Ref.7, ECO:0007744\|PDB:3PQD

Chain	Residue	Details
A	HIS178
B	HIS178
C	HIS178
D	HIS178
E	HIS178
F	HIS178
G	HIS178
H	HIS178

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269\|Ref.7, ECO:0007744\|PDB:3PQD, ECO:0007744\|PDB:3PQF

Chain	Residue	Details
A	PHE15
B	PHE15
C	PHE15
D	PHE15
E	PHE15
F	PHE15
G	PHE15
H	PHE15

site_id	SWS_FT_FI3
Number of Residues	16
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00488, ECO:0000269\|Ref.7, ECO:0007744\|PDB:3PQF

Chain	Residue	Details
A	ASP37
E	GLY82
F	ASP37
F	GLY82
G	ASP37
G	GLY82
H	ASP37
H	GLY82
A	GLY82
B	ASP37
B	GLY82
C	ASP37
C	GLY82
D	ASP37
D	GLY82
E	ASP37

site_id	SWS_FT_FI4
Number of Residues	64
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00488

Chain	Residue	Details
A	LYS42
B	TYR68
B	GLN85
B	ARG91
B	ASN123
B	SER146
B	ASP151
B	THR232
C	LYS42
C	TYR68
C	GLN85
A	TYR68
C	ARG91
C	ASN123
C	SER146
C	ASP151
C	THR232
D	LYS42
D	TYR68
D	GLN85
D	ARG91
D	ASN123
A	GLN85
D	SER146
D	ASP151
D	THR232
E	LYS42
E	TYR68
E	GLN85
E	ARG91
E	ASN123
E	SER146
E	ASP151
A	ARG91
E	THR232
F	LYS42
F	TYR68
F	GLN85
F	ARG91
F	ASN123
F	SER146
F	ASP151
F	THR232
G	LYS42
A	ASN123
G	TYR68
G	GLN85
G	ARG91
G	ASN123
G	SER146
G	ASP151
G	THR232
H	LYS42
H	TYR68
H	GLN85
A	SER146
H	ARG91
H	ASN123
H	SER146
H	ASP151
H	THR232
A	ASP151
A	THR232
B	LYS42

site_id	SWS_FT_FI5
Number of Residues	8
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00488, ECO:0000305\|Ref.7, ECO:0007744\|PDB:3PQD

Chain	Residue	Details
A	ALA121
B	ALA121
C	ALA121
D	ALA121
E	ALA121
F	ALA121
G	ALA121
H	ALA121

site_id	SWS_FT_FI6
Number of Residues	8
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00488, ECO:0000269\|Ref.7, ECO:0007744\|PDB:3PQD

Chain	Residue	Details
A	ARG156
B	ARG156
C	ARG156
D	ARG156
E	ARG156
F	ARG156
G	ARG156
H	ARG156

site_id	SWS_FT_FI7
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269\|Ref.7, ECO:0007744\|PDB:3PQD

Chain	Residue	Details
A	GLN168
B	GLN168
C	GLN168
D	GLN168
E	GLN168
F	GLN168
G	GLN168
H	GLN168

site_id	SWS_FT_FI8
Number of Residues	8
Details	MOD_RES: Phosphotyrosine => ECO:0000255\|HAMAP-Rule:MF_00488, ECO:0000269\|PubMed:17218307

Chain	Residue	Details
A	TYR223
B	TYR223
C	TYR223
D	TYR223
E	TYR223
F	TYR223
G	TYR223
H	TYR223

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)