3PNS
Crystal Structure of Uridine Phosphorylase Complexed with Uracil from Vibrio cholerae O1 biovar El Tor
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004850 | molecular_function | uridine phosphorylase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0009116 | biological_process | nucleoside metabolic process |
| A | 0009164 | biological_process | nucleoside catabolic process |
| A | 0009166 | biological_process | nucleotide catabolic process |
| A | 0016757 | molecular_function | glycosyltransferase activity |
| A | 0016763 | molecular_function | pentosyltransferase activity |
| A | 0044206 | biological_process | UMP salvage |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004850 | molecular_function | uridine phosphorylase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0009116 | biological_process | nucleoside metabolic process |
| B | 0009164 | biological_process | nucleoside catabolic process |
| B | 0009166 | biological_process | nucleotide catabolic process |
| B | 0016757 | molecular_function | glycosyltransferase activity |
| B | 0016763 | molecular_function | pentosyltransferase activity |
| B | 0044206 | biological_process | UMP salvage |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004850 | molecular_function | uridine phosphorylase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0009116 | biological_process | nucleoside metabolic process |
| C | 0009164 | biological_process | nucleoside catabolic process |
| C | 0009166 | biological_process | nucleotide catabolic process |
| C | 0016757 | molecular_function | glycosyltransferase activity |
| C | 0016763 | molecular_function | pentosyltransferase activity |
| C | 0044206 | biological_process | UMP salvage |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004850 | molecular_function | uridine phosphorylase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0009116 | biological_process | nucleoside metabolic process |
| D | 0009164 | biological_process | nucleoside catabolic process |
| D | 0009166 | biological_process | nucleotide catabolic process |
| D | 0016757 | molecular_function | glycosyltransferase activity |
| D | 0016763 | molecular_function | pentosyltransferase activity |
| D | 0044206 | biological_process | UMP salvage |
| E | 0003824 | molecular_function | catalytic activity |
| E | 0004850 | molecular_function | uridine phosphorylase activity |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0005829 | cellular_component | cytosol |
| E | 0009116 | biological_process | nucleoside metabolic process |
| E | 0009164 | biological_process | nucleoside catabolic process |
| E | 0009166 | biological_process | nucleotide catabolic process |
| E | 0016757 | molecular_function | glycosyltransferase activity |
| E | 0016763 | molecular_function | pentosyltransferase activity |
| E | 0044206 | biological_process | UMP salvage |
| F | 0003824 | molecular_function | catalytic activity |
| F | 0004850 | molecular_function | uridine phosphorylase activity |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0005829 | cellular_component | cytosol |
| F | 0009116 | biological_process | nucleoside metabolic process |
| F | 0009164 | biological_process | nucleoside catabolic process |
| F | 0009166 | biological_process | nucleotide catabolic process |
| F | 0016757 | molecular_function | glycosyltransferase activity |
| F | 0016763 | molecular_function | pentosyltransferase activity |
| F | 0044206 | biological_process | UMP salvage |
| G | 0003824 | molecular_function | catalytic activity |
| G | 0004850 | molecular_function | uridine phosphorylase activity |
| G | 0005737 | cellular_component | cytoplasm |
| G | 0005829 | cellular_component | cytosol |
| G | 0009116 | biological_process | nucleoside metabolic process |
| G | 0009164 | biological_process | nucleoside catabolic process |
| G | 0009166 | biological_process | nucleotide catabolic process |
| G | 0016757 | molecular_function | glycosyltransferase activity |
| G | 0016763 | molecular_function | pentosyltransferase activity |
| G | 0044206 | biological_process | UMP salvage |
| H | 0003824 | molecular_function | catalytic activity |
| H | 0004850 | molecular_function | uridine phosphorylase activity |
| H | 0005737 | cellular_component | cytoplasm |
| H | 0005829 | cellular_component | cytosol |
| H | 0009116 | biological_process | nucleoside metabolic process |
| H | 0009164 | biological_process | nucleoside catabolic process |
| H | 0009166 | biological_process | nucleotide catabolic process |
| H | 0016757 | molecular_function | glycosyltransferase activity |
| H | 0016763 | molecular_function | pentosyltransferase activity |
| H | 0044206 | biological_process | UMP salvage |
| I | 0003824 | molecular_function | catalytic activity |
| I | 0004850 | molecular_function | uridine phosphorylase activity |
| I | 0005737 | cellular_component | cytoplasm |
| I | 0005829 | cellular_component | cytosol |
| I | 0009116 | biological_process | nucleoside metabolic process |
| I | 0009164 | biological_process | nucleoside catabolic process |
| I | 0009166 | biological_process | nucleotide catabolic process |
| I | 0016757 | molecular_function | glycosyltransferase activity |
| I | 0016763 | molecular_function | pentosyltransferase activity |
| I | 0044206 | biological_process | UMP salvage |
| J | 0003824 | molecular_function | catalytic activity |
| J | 0004850 | molecular_function | uridine phosphorylase activity |
| J | 0005737 | cellular_component | cytoplasm |
| J | 0005829 | cellular_component | cytosol |
| J | 0009116 | biological_process | nucleoside metabolic process |
| J | 0009164 | biological_process | nucleoside catabolic process |
| J | 0009166 | biological_process | nucleotide catabolic process |
| J | 0016757 | molecular_function | glycosyltransferase activity |
| J | 0016763 | molecular_function | pentosyltransferase activity |
| J | 0044206 | biological_process | UMP salvage |
| K | 0003824 | molecular_function | catalytic activity |
| K | 0004850 | molecular_function | uridine phosphorylase activity |
| K | 0005737 | cellular_component | cytoplasm |
| K | 0005829 | cellular_component | cytosol |
| K | 0009116 | biological_process | nucleoside metabolic process |
| K | 0009164 | biological_process | nucleoside catabolic process |
| K | 0009166 | biological_process | nucleotide catabolic process |
| K | 0016757 | molecular_function | glycosyltransferase activity |
| K | 0016763 | molecular_function | pentosyltransferase activity |
| K | 0044206 | biological_process | UMP salvage |
| L | 0003824 | molecular_function | catalytic activity |
| L | 0004850 | molecular_function | uridine phosphorylase activity |
| L | 0005737 | cellular_component | cytoplasm |
| L | 0005829 | cellular_component | cytosol |
| L | 0009116 | biological_process | nucleoside metabolic process |
| L | 0009164 | biological_process | nucleoside catabolic process |
| L | 0009166 | biological_process | nucleotide catabolic process |
| L | 0016757 | molecular_function | glycosyltransferase activity |
| L | 0016763 | molecular_function | pentosyltransferase activity |
| L | 0044206 | biological_process | UMP salvage |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL A 260 |
| Chain | Residue |
| A | HIS12 |
| A | ARG52 |
| A | HOH280 |
| B | PHE166 |
| B | GLU202 |
| B | URA271 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL A 270 |
| Chain | Residue |
| K | PRO129 |
| K | GLU131 |
| L | ARG182 |
| A | ARG183 |
| A | GLU190 |
| A | HOH892 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL B 261 |
| Chain | Residue |
| A | PHE166 |
| A | GLU202 |
| B | HIS12 |
| B | HOH400 |
| B | HOH508 |
| B | HOH1470 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL B 270 |
| Chain | Residue |
| B | ARG183 |
| B | GLU190 |
| G | ARG182 |
| H | GLU131 |
| H | HOH614 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE URA B 271 |
| Chain | Residue |
| A | GOL260 |
| B | GLN170 |
| B | ARG172 |
| B | MSE201 |
| B | HOH445 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL C 261 |
| Chain | Residue |
| C | GLU202 |
| C | URA276 |
| C | HOH1866 |
| D | HIS12 |
| D | ARG52 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL C 271 |
| Chain | Residue |
| C | ARG183 |
| C | GLU190 |
| C | HOH1561 |
| I | GLU131 |
| J | ARG182 |
| J | HOH1203 |
| site_id | AC8 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CL C 274 |
| Chain | Residue |
| C | GLY214 |
| C | LYS216 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL C 275 |
| Chain | Residue |
| C | ARG172 |
| C | TYR173 |
| C | ASP174 |
| C | HOH1557 |
| C | HOH1689 |
| site_id | BC1 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE URA C 276 |
| Chain | Residue |
| C | GLY100 |
| C | PHE166 |
| C | GLN170 |
| C | ARG172 |
| C | GLU200 |
| C | MSE201 |
| C | GOL261 |
| C | HOH553 |
| site_id | BC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 C 259 |
| Chain | Residue |
| C | ARG182 |
| C | HOH1611 |
| F | ARG182 |
| J | ARG182 |
| site_id | BC3 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL D 260 |
| Chain | Residue |
| C | HIS12 |
| C | ARG52 |
| C | HOH1869 |
| D | GLU202 |
| D | URA270 |
| D | HOH308 |
| D | HOH1323 |
| D | HOH1870 |
| site_id | BC4 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE URA D 270 |
| Chain | Residue |
| D | GLY100 |
| D | PHE166 |
| D | GLN170 |
| D | ARG172 |
| D | GLU200 |
| D | MSE201 |
| D | GOL260 |
| D | HOH426 |
| D | HOH1169 |
| site_id | BC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL D 271 |
| Chain | Residue |
| D | ARG183 |
| D | GLU190 |
| D | HOH644 |
| E | ARG182 |
| F | GLU131 |
| site_id | BC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CL E 270 |
| Chain | Residue |
| E | ARG179 |
| F | PHE127 |
| F | HOH259 |
| F | HOH674 |
| F | HOH827 |
| site_id | BC7 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE URA E 271 |
| Chain | Residue |
| E | THR99 |
| E | GLY100 |
| E | PHE166 |
| E | GLN170 |
| E | ARG172 |
| E | GLU200 |
| E | MSE201 |
| E | GOL272 |
| E | HOH367 |
| site_id | BC8 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE FMT E 273 |
| Chain | Residue |
| E | HOH1940 |
| F | ARG52 |
| E | PRO29 |
| E | GLY30 |
| E | ASP31 |
| E | VAL96 |
| E | GLY97 |
| site_id | BC9 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL E 272 |
| Chain | Residue |
| E | ILE73 |
| E | THR98 |
| E | GLU202 |
| E | URA271 |
| E | HOH557 |
| F | HIS12 |
| F | ARG52 |
| site_id | CC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL E 259 |
| Chain | Residue |
| E | ARG183 |
| E | GLU190 |
| E | HOH324 |
| E | HOH704 |
| I | ARG182 |
| J | GLU131 |
| site_id | CC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL F 270 |
| Chain | Residue |
| C | ARG182 |
| D | GLU131 |
| D | HOH1889 |
| D | HOH1890 |
| F | ARG183 |
| F | GLU190 |
| site_id | CC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL F 273 |
| Chain | Residue |
| E | HIS12 |
| F | MSE201 |
| F | GLU202 |
| F | URA274 |
| site_id | CC4 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE URA F 274 |
| Chain | Residue |
| F | THR99 |
| F | GLY100 |
| F | GLN170 |
| F | ARG172 |
| F | GLU200 |
| F | MSE201 |
| F | ILE225 |
| F | GOL273 |
| F | HOH2009 |
| site_id | CC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ACY F 275 |
| Chain | Residue |
| F | ARG172 |
| F | TYR173 |
| F | ASP174 |
| F | HOH1131 |
| site_id | CC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 G 270 |
| Chain | Residue |
| B | ARG182 |
| G | ARG182 |
| G | HOH1964 |
| K | ARG182 |
| site_id | CC7 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL G 271 |
| Chain | Residue |
| G | THR98 |
| G | PHE166 |
| G | MSE201 |
| G | GLU202 |
| G | URA273 |
| G | HOH2004 |
| H | HIS12 |
| site_id | CC8 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL G 272 |
| Chain | Residue |
| G | PRO129 |
| G | GLU131 |
| G | HOH420 |
| G | HOH1273 |
| H | ARG182 |
| L | ARG183 |
| L | GLU190 |
| site_id | CC9 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE URA G 273 |
| Chain | Residue |
| G | THR99 |
| G | GLY100 |
| G | PHE166 |
| G | GLN170 |
| G | ARG172 |
| G | GLU200 |
| G | MSE201 |
| G | ILE225 |
| G | GOL271 |
| G | HOH281 |
| site_id | DC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL G 274 |
| Chain | Residue |
| G | ARG183 |
| G | GLU190 |
| G | HOH607 |
| K | ARG182 |
| L | PRO129 |
| L | GLU131 |
| site_id | DC2 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE URA H 270 |
| Chain | Residue |
| H | THR98 |
| H | THR99 |
| H | GLY100 |
| H | PHE166 |
| H | GLN170 |
| H | ARG172 |
| H | GLU200 |
| H | MSE201 |
| H | ILE225 |
| H | GOL271 |
| H | HOH362 |
| site_id | DC3 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL H 271 |
| Chain | Residue |
| G | HIS12 |
| G | ARG52 |
| H | ILE73 |
| H | THR98 |
| H | PHE166 |
| H | GLU202 |
| H | URA270 |
| H | HOH1049 |
| H | HOH1054 |
| site_id | DC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE FMT H 272 |
| Chain | Residue |
| G | HOH2004 |
| H | PHE11 |
| H | HIS51 |
| H | ARG52 |
| site_id | DC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL I 270 |
| Chain | Residue |
| C | GLU131 |
| D | ARG182 |
| I | ARG183 |
| I | GLU190 |
| I | HOH282 |
| I | HOH774 |
| site_id | DC6 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE URA I 259 |
| Chain | Residue |
| I | THR99 |
| I | GLY100 |
| I | PHE166 |
| I | GLN170 |
| I | ARG172 |
| I | GLU200 |
| I | MSE201 |
| I | ILE225 |
| I | HOH266 |
| I | GOL272 |
| site_id | DC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 I 271 |
| Chain | Residue |
| D | ARG182 |
| E | ARG182 |
| I | ARG182 |
| I | HOH1931 |
| site_id | DC8 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL I 272 |
| Chain | Residue |
| I | THR98 |
| I | PHE166 |
| I | MSE201 |
| I | GLU202 |
| I | URA259 |
| I | HOH819 |
| J | HIS12 |
| site_id | DC9 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CL I 273 |
| Chain | Residue |
| I | ARG52 |
| site_id | EC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CL I 274 |
| Chain | Residue |
| I | TYR173 |
| I | HOH1006 |
| site_id | EC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL J 270 |
| Chain | Residue |
| E | PRO129 |
| E | GLU131 |
| F | ARG182 |
| J | ARG183 |
| J | GLU190 |
| J | HOH260 |
| J | HOH1920 |
| site_id | EC3 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE URA J 271 |
| Chain | Residue |
| J | GLY100 |
| J | PHE166 |
| J | GLN170 |
| J | ARG172 |
| J | GLU200 |
| J | MSE201 |
| J | ILE225 |
| J | GOL273 |
| J | HOH431 |
| site_id | EC4 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL J 273 |
| Chain | Residue |
| I | HIS12 |
| I | ARG52 |
| J | ILE73 |
| J | THR98 |
| J | PHE166 |
| J | GLU202 |
| J | URA271 |
| J | HOH1803 |
| site_id | EC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL K 271 |
| Chain | Residue |
| A | GLU131 |
| B | ARG182 |
| K | ARG183 |
| K | GLU190 |
| K | HOH823 |
| K | HOH1041 |
| site_id | EC6 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE URA K 272 |
| Chain | Residue |
| K | THR98 |
| K | THR99 |
| K | GLY100 |
| K | PHE166 |
| K | GLN170 |
| K | ARG172 |
| K | GLU200 |
| K | MSE201 |
| K | ILE225 |
| K | GOL270 |
| K | HOH281 |
| site_id | EC7 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL K 270 |
| Chain | Residue |
| K | THR98 |
| K | MSE201 |
| K | GLU202 |
| K | URA272 |
| K | HOH1149 |
| K | HOH1428 |
| L | HIS12 |
| site_id | EC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL L 270 |
| Chain | Residue |
| K | HIS12 |
| L | PHE166 |
| L | GLU202 |
| L | URA271 |
| L | HOH989 |
| site_id | EC9 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE URA L 271 |
| Chain | Residue |
| L | GLY100 |
| L | PHE166 |
| L | GLN170 |
| L | ARG172 |
| L | GLU200 |
| L | MSE201 |
| L | GOL270 |
| L | HOH593 |
Functional Information from PROSITE/UniProt
| site_id | PS01232 |
| Number of Residues | 16 |
| Details | PNP_UDP_1 Purine and other phosphorylases family 1 signature. StGIGgPStSIaveEL |
| Chain | Residue | Details |
| A | SER70-LEU85 |
