3PGJ

2.49 Angstrom resolution crystal structure of shikimate 5-dehydrogenase (aroE) from Vibrio cholerae O1 biovar eltor str. N16961 in complex with shikimate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003674	molecular_function	molecular_function
A	0004764	molecular_function	shikimate 3-dehydrogenase (NADP+) activity
A	0005575	cellular_component	cellular_component
A	0005829	cellular_component	cytosol
A	0008150	biological_process	biological_process
A	0008652	biological_process	amino acid biosynthetic process
A	0009073	biological_process	aromatic amino acid family biosynthetic process
A	0009423	biological_process	chorismate biosynthetic process
A	0016491	molecular_function	oxidoreductase activity
A	0019632	biological_process	shikimate metabolic process
A	0050661	molecular_function	NADP binding
B	0003674	molecular_function	molecular_function
B	0004764	molecular_function	shikimate 3-dehydrogenase (NADP+) activity
B	0005575	cellular_component	cellular_component
B	0005829	cellular_component	cytosol
B	0008150	biological_process	biological_process
B	0008652	biological_process	amino acid biosynthetic process
B	0009073	biological_process	aromatic amino acid family biosynthetic process
B	0009423	biological_process	chorismate biosynthetic process
B	0016491	molecular_function	oxidoreductase activity
B	0019632	biological_process	shikimate metabolic process
B	0050661	molecular_function	NADP binding
C	0003674	molecular_function	molecular_function
C	0004764	molecular_function	shikimate 3-dehydrogenase (NADP+) activity
C	0005575	cellular_component	cellular_component
C	0005829	cellular_component	cytosol
C	0008150	biological_process	biological_process
C	0008652	biological_process	amino acid biosynthetic process
C	0009073	biological_process	aromatic amino acid family biosynthetic process
C	0009423	biological_process	chorismate biosynthetic process
C	0016491	molecular_function	oxidoreductase activity
C	0019632	biological_process	shikimate metabolic process
C	0050661	molecular_function	NADP binding
D	0003674	molecular_function	molecular_function
D	0004764	molecular_function	shikimate 3-dehydrogenase (NADP+) activity
D	0005575	cellular_component	cellular_component
D	0005829	cellular_component	cytosol
D	0008150	biological_process	biological_process
D	0008652	biological_process	amino acid biosynthetic process
D	0009073	biological_process	aromatic amino acid family biosynthetic process
D	0009423	biological_process	chorismate biosynthetic process
D	0016491	molecular_function	oxidoreductase activity
D	0019632	biological_process	shikimate metabolic process
D	0050661	molecular_function	NADP binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SKM A 279

Chain	Residue
A	SER18
A	SER20
A	ASN63
A	THR65
A	LYS69
A	ASN90
A	ASP106
A	GLN248
A	HOH287

---

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE SKM B 279

Chain	Residue
B	VAL10
B	SER18
B	SER20
B	ASN63
B	THR65
B	LYS69
B	ASN90
B	ASP106
B	GLN248
B	HOH297

---

site_id	AC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SKM C 279

Chain	Residue
C	VAL10
C	SER18
C	SER20
C	ASN63
C	THR65
C	LYS69
C	ASN90
C	ASP106
C	GLN248

---

site_id	AC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE SKM D 279

Chain	Residue
D	VAL10
D	SER18
D	SER20
D	THR65
D	LYS69
D	ASN90
D	ASP106
D	GLN248

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_00222, ECO:0000305|Ref.2, ECO:0000305|Ref.3

Chain	Residue	Details
A	LYS69
B	LYS69
C	LYS69
D	LYS69

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00222, ECO:0000269|Ref.2, ECO:0000269|Ref.3

Chain	Residue	Details
A	SER18
A	ASP106
B	SER18
B	ASP106
C	SER18
C	ASP106
D	SER18
D	ASP106

---

site_id	SWS_FT_FI3
Number of Residues	20
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00222

Chain	Residue	Details
A	THR65
B	GLY241
C	THR65
C	GLU81
C	GLY130
C	TYR223
C	GLY241
D	THR65
D	GLU81
D	GLY130
D	TYR223
A	GLU81
D	GLY241
A	GLY130
A	TYR223
A	GLY241
B	THR65
B	GLU81
B	GLY130
B	TYR223

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00222, ECO:0000269|Ref.2

Chain	Residue	Details
A	ASN90
A	GLN248
B	ASN90
B	GLN248
C	ASN90
C	GLN248
D	ASN90
D	GLN248

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00222, ECO:0000269|Ref.3

Chain	Residue	Details
A	ASN154
B	ASN154
C	ASN154
D	ASN154

---