3PGJ
2.49 Angstrom resolution crystal structure of shikimate 5-dehydrogenase (aroE) from Vibrio cholerae O1 biovar eltor str. N16961 in complex with shikimate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004764 | molecular_function | shikimate 3-dehydrogenase (NADP+) activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0008652 | biological_process | amino acid biosynthetic process |
| A | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| A | 0009423 | biological_process | chorismate biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0019632 | biological_process | shikimate metabolic process |
| A | 0050661 | molecular_function | NADP binding |
| B | 0004764 | molecular_function | shikimate 3-dehydrogenase (NADP+) activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0008652 | biological_process | amino acid biosynthetic process |
| B | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| B | 0009423 | biological_process | chorismate biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0019632 | biological_process | shikimate metabolic process |
| B | 0050661 | molecular_function | NADP binding |
| C | 0004764 | molecular_function | shikimate 3-dehydrogenase (NADP+) activity |
| C | 0005829 | cellular_component | cytosol |
| C | 0008652 | biological_process | amino acid biosynthetic process |
| C | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| C | 0009423 | biological_process | chorismate biosynthetic process |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0019632 | biological_process | shikimate metabolic process |
| C | 0050661 | molecular_function | NADP binding |
| D | 0004764 | molecular_function | shikimate 3-dehydrogenase (NADP+) activity |
| D | 0005829 | cellular_component | cytosol |
| D | 0008652 | biological_process | amino acid biosynthetic process |
| D | 0009073 | biological_process | aromatic amino acid family biosynthetic process |
| D | 0009423 | biological_process | chorismate biosynthetic process |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0019632 | biological_process | shikimate metabolic process |
| D | 0050661 | molecular_function | NADP binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE SKM A 279 |
| Chain | Residue |
| A | SER18 |
| A | SER20 |
| A | ASN63 |
| A | THR65 |
| A | LYS69 |
| A | ASN90 |
| A | ASP106 |
| A | GLN248 |
| A | HOH287 |
| site_id | AC2 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE SKM B 279 |
| Chain | Residue |
| B | VAL10 |
| B | SER18 |
| B | SER20 |
| B | ASN63 |
| B | THR65 |
| B | LYS69 |
| B | ASN90 |
| B | ASP106 |
| B | GLN248 |
| B | HOH297 |
| site_id | AC3 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE SKM C 279 |
| Chain | Residue |
| C | VAL10 |
| C | SER18 |
| C | SER20 |
| C | ASN63 |
| C | THR65 |
| C | LYS69 |
| C | ASN90 |
| C | ASP106 |
| C | GLN248 |
| site_id | AC4 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE SKM D 279 |
| Chain | Residue |
| D | VAL10 |
| D | SER18 |
| D | SER20 |
| D | THR65 |
| D | LYS69 |
| D | ASN90 |
| D | ASP106 |
| D | GLN248 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_00222, ECO:0000305|Ref.2, ECO:0000305|Ref.3 |
| Chain | Residue | Details |
| A | LYS69 | |
| B | LYS69 | |
| C | LYS69 | |
| D | LYS69 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00222, ECO:0000269|Ref.2, ECO:0000269|Ref.3 |
| Chain | Residue | Details |
| A | SER18 | |
| A | ASP106 | |
| B | SER18 | |
| B | ASP106 | |
| C | SER18 | |
| C | ASP106 | |
| D | SER18 | |
| D | ASP106 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 20 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00222 |
| Chain | Residue | Details |
| A | THR65 | |
| B | GLY241 | |
| C | THR65 | |
| C | GLU81 | |
| C | GLY130 | |
| C | TYR223 | |
| C | GLY241 | |
| D | THR65 | |
| D | GLU81 | |
| D | GLY130 | |
| D | TYR223 | |
| A | GLU81 | |
| D | GLY241 | |
| A | GLY130 | |
| A | TYR223 | |
| A | GLY241 | |
| B | THR65 | |
| B | GLU81 | |
| B | GLY130 | |
| B | TYR223 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00222, ECO:0000269|Ref.2 |
| Chain | Residue | Details |
| A | ASN90 | |
| A | GLN248 | |
| B | ASN90 | |
| B | GLN248 | |
| C | ASN90 | |
| C | GLN248 | |
| D | ASN90 | |
| D | GLN248 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00222, ECO:0000269|Ref.3 |
| Chain | Residue | Details |
| A | ASN154 | |
| B | ASN154 | |
| C | ASN154 | |
| D | ASN154 |
