Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3OZE

Crystal Structure of human 5'-deoxy-5'-methyladenosine phosphorylase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004645	molecular_function	1,4-alpha-oligoglucan phosphorylase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006139	biological_process	nucleobase-containing compound metabolic process
A	0006166	biological_process	purine ribonucleoside salvage
A	0006738	biological_process	nicotinamide riboside catabolic process
A	0009116	biological_process	nucleoside metabolic process
A	0016757	molecular_function	glycosyltransferase activity
A	0016763	molecular_function	pentosyltransferase activity
A	0017061	molecular_function	S-methyl-5-thioadenosine phosphorylase activity
A	0019509	biological_process	L-methionine salvage from methylthioadenosine
A	0032259	biological_process	methylation
A	0033574	biological_process	response to testosterone
A	0070062	cellular_component	extracellular exosome
B	0003824	molecular_function	catalytic activity
B	0004645	molecular_function	1,4-alpha-oligoglucan phosphorylase activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006139	biological_process	nucleobase-containing compound metabolic process
B	0006166	biological_process	purine ribonucleoside salvage
B	0006738	biological_process	nicotinamide riboside catabolic process
B	0009116	biological_process	nucleoside metabolic process
B	0016757	molecular_function	glycosyltransferase activity
B	0016763	molecular_function	pentosyltransferase activity
B	0017061	molecular_function	S-methyl-5-thioadenosine phosphorylase activity
B	0019509	biological_process	L-methionine salvage from methylthioadenosine
B	0032259	biological_process	methylation
B	0033574	biological_process	response to testosterone
B	0070062	cellular_component	extracellular exosome
C	0003824	molecular_function	catalytic activity
C	0004645	molecular_function	1,4-alpha-oligoglucan phosphorylase activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006139	biological_process	nucleobase-containing compound metabolic process
C	0006166	biological_process	purine ribonucleoside salvage
C	0006738	biological_process	nicotinamide riboside catabolic process
C	0009116	biological_process	nucleoside metabolic process
C	0016757	molecular_function	glycosyltransferase activity
C	0016763	molecular_function	pentosyltransferase activity
C	0017061	molecular_function	S-methyl-5-thioadenosine phosphorylase activity
C	0019509	biological_process	L-methionine salvage from methylthioadenosine
C	0032259	biological_process	methylation
C	0033574	biological_process	response to testosterone
C	0070062	cellular_component	extracellular exosome
D	0003824	molecular_function	catalytic activity
D	0004645	molecular_function	1,4-alpha-oligoglucan phosphorylase activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006139	biological_process	nucleobase-containing compound metabolic process
D	0006166	biological_process	purine ribonucleoside salvage
D	0006738	biological_process	nicotinamide riboside catabolic process
D	0009116	biological_process	nucleoside metabolic process
D	0016757	molecular_function	glycosyltransferase activity
D	0016763	molecular_function	pentosyltransferase activity
D	0017061	molecular_function	S-methyl-5-thioadenosine phosphorylase activity
D	0019509	biological_process	L-methionine salvage from methylthioadenosine
D	0032259	biological_process	methylation
D	0033574	biological_process	response to testosterone
D	0070062	cellular_component	extracellular exosome
E	0003824	molecular_function	catalytic activity
E	0004645	molecular_function	1,4-alpha-oligoglucan phosphorylase activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006139	biological_process	nucleobase-containing compound metabolic process
E	0006166	biological_process	purine ribonucleoside salvage
E	0006738	biological_process	nicotinamide riboside catabolic process
E	0009116	biological_process	nucleoside metabolic process
E	0016757	molecular_function	glycosyltransferase activity
E	0016763	molecular_function	pentosyltransferase activity
E	0017061	molecular_function	S-methyl-5-thioadenosine phosphorylase activity
E	0019509	biological_process	L-methionine salvage from methylthioadenosine
E	0032259	biological_process	methylation
E	0033574	biological_process	response to testosterone
E	0070062	cellular_component	extracellular exosome
F	0003824	molecular_function	catalytic activity
F	0004645	molecular_function	1,4-alpha-oligoglucan phosphorylase activity
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006139	biological_process	nucleobase-containing compound metabolic process
F	0006166	biological_process	purine ribonucleoside salvage
F	0006738	biological_process	nicotinamide riboside catabolic process
F	0009116	biological_process	nucleoside metabolic process
F	0016757	molecular_function	glycosyltransferase activity
F	0016763	molecular_function	pentosyltransferase activity
F	0017061	molecular_function	S-methyl-5-thioadenosine phosphorylase activity
F	0019509	biological_process	L-methionine salvage from methylthioadenosine
F	0032259	biological_process	methylation
F	0033574	biological_process	response to testosterone
F	0070062	cellular_component	extracellular exosome

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 A 284

Chain	Residue
A	GLY17
A	HOH313
A	THR18
A	ARG60
A	HIS61
A	THR93
A	ALA94
A	THR197
A	HOH286
A	HOH294

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PO4 B 284

Chain	Residue
B	GLY17
B	THR18
B	ARG60
B	HIS61
B	THR93
B	ALA94
B	THR197
B	HOH289
B	HOH302
B	HOH313

site_id	AC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PO4 C 284

Chain	Residue
C	GLY17
C	THR18
C	ARG60
C	HIS61
C	THR93
C	ALA94
C	THR197
C	HOH307
C	HOH309

site_id	AC4
Number of Residues	11
Details	BINDING SITE FOR RESIDUE PO4 D 284

Chain	Residue
D	GLY17
D	THR18
D	ARG60
D	HIS61
D	THR93
D	ALA94
D	THR197
D	HOH290
D	HOH298
D	HOH305
D	HOH317

site_id	AC5
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PO4 E 284

Chain	Residue
E	GLY17
E	THR18
E	ARG60
E	HIS61
E	THR93
E	ALA94
E	THR197
E	HOH303
E	HOH325

site_id	AC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PO4 F 284

Chain	Residue
F	GLY17
F	THR18
F	ARG60
F	HIS61
F	THR93
F	ALA94
F	THR197
F	HOH303
F	HOH321

Functional Information from PROSITE/UniProt

site_id	PS01240
Number of Residues	41
Details	PNP_MTAP_2 Purine and other phosphorylases family 2 signature. LarhGrqHtImpskVnyqAn.IwAlkeeGcth.VIvtTAcGSL

Chain	Residue	Details
A	LEU58-LEU98

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	36
Details	BINDING:

Chain	Residue	Details
A	THR18
B	MET196
B	THR197
B	ASP220
C	THR18
C	ARG60
C	THR93
C	MET196
C	THR197
C	ASP220
D	THR18
A	ARG60
D	ARG60
D	THR93
D	MET196
D	THR197
D	ASP220
E	THR18
E	ARG60
E	THR93
E	MET196
E	THR197
A	THR93
E	ASP220
F	THR18
F	ARG60
F	THR93
F	MET196
F	THR197
F	ASP220
A	MET196
A	THR197
A	ASP220
B	THR18
B	ARG60
B	THR93

site_id	SWS_FT_FI2
Number of Residues	12
Details	SITE: Important for substrate specificity

Chain	Residue	Details
A	SER178
E	VAL233
F	SER178
F	VAL233
A	VAL233
B	SER178
B	VAL233
C	SER178
C	VAL233
D	SER178
D	VAL233
E	SER178

site_id	SWS_FT_FI3
Number of Residues	6
Details	MOD_RES: N6-acetyllysine => ECO:0000250\|UniProtKB:Q9CQ65

Chain	Residue	Details
A	LYS51
B	LYS51
C	LYS51
D	LYS51
E	LYS51
F	LYS51

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	2
Details	M-CSA 244

Chain	Residue	Details
A	ASP220	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	ASP222	electrostatic stabiliser

site_id	MCSA2
Number of Residues	2
Details	M-CSA 244

Chain	Residue	Details
B	ASP220	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
B	ASP222	electrostatic stabiliser

site_id	MCSA3
Number of Residues	2
Details	M-CSA 244

Chain	Residue	Details
C	ASP220	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
C	ASP222	electrostatic stabiliser

site_id	MCSA4
Number of Residues	2
Details	M-CSA 244

Chain	Residue	Details
D	ASP220	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
D	ASP222	electrostatic stabiliser

site_id	MCSA5
Number of Residues	2
Details	M-CSA 244

Chain	Residue	Details
E	ASP220	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
E	ASP222	electrostatic stabiliser

site_id	MCSA6
Number of Residues	2
Details	M-CSA 244

Chain	Residue	Details
F	ASP220	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
F	ASP222	electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)