3OW5

Crystal structure of the Y200A mutant of gamma carbonic anhydrase from Methanosarcina thermophila

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004089	molecular_function	carbonate dehydratase activity
A	0005576	cellular_component	extracellular region
A	0008270	molecular_function	zinc ion binding
A	0016829	molecular_function	lyase activity
A	0043199	molecular_function	sulfate binding
A	0046872	molecular_function	metal ion binding
A	0050897	molecular_function	cobalt ion binding
A	0071890	molecular_function	bicarbonate binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN A 214

Chain	Residue
A	HIS81
A	HIS117
A	HIS122
A	HOH282
A	BCT301
A	SO4302

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FE A 215

Chain	Residue
A	HOH226
A	HOH226
A	HOH226
A	MET55
A	MET55
A	MET55

---

site_id	AC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 A 302

Chain	Residue
A	GLU62
A	GLN75
A	HIS81
A	HIS122
A	VAL198
A	ASN202
A	ZN214
A	HOH219
A	HOH282

---

site_id	AC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE BCT A 301

Chain	Residue
A	GLU62
A	GLN75
A	HIS81
A	ALA82
A	LEU83
A	HIS117
A	HIS122
A	ZN214
A	HOH282

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton donor/acceptor => ECO:0000305|PubMed:10924115

Chain	Residue	Details
A	GLU62

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: ACT_SITE => ECO:0000305|PubMed:10924115

Chain	Residue	Details
A	GLU84

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269|PubMed:10924115

Chain	Residue	Details
A	ARG59
A	GLN75
A	ASN202

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269|PubMed:10924115, ECO:0000269|PubMed:8665839, ECO:0007744|PDB:1QRG, ECO:0007744|PDB:1QRL, ECO:0007744|PDB:1QRM, ECO:0007744|PDB:1THJ

Chain	Residue	Details
A	HIS81
A	HIS117
A	HIS122

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	7
Details	M-CSA 516

Chain	Residue	Details
A	GLU62	electrostatic destabiliser, electrostatic stabiliser, proton acceptor, proton donor
A	GLN75	electrostatic stabiliser
A	HIS81	metal ligand
A	GLU84	proton acceptor
A	HIS117	metal ligand
A	HIS122	metal ligand
A	ASN202	electrostatic stabiliser, increase electrophilicity

---