3O61

Structure of the E100A E.coli GDP-mannose hydrolase (yffh) in complex with GDP-mannose and Mg++

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003824	molecular_function	catalytic activity
A	0005829	cellular_component	cytosol
A	0006753	biological_process	nucleoside phosphate metabolic process
A	0016787	molecular_function	hydrolase activity
A	0016818	molecular_function	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
A	0019693	biological_process	ribose phosphate metabolic process
A	0042803	molecular_function	protein homodimerization activity
A	0046872	molecular_function	metal ion binding
A	0052751	molecular_function	GDP-mannose hydrolase activity
B	0000287	molecular_function	magnesium ion binding
B	0003824	molecular_function	catalytic activity
B	0005829	cellular_component	cytosol
B	0006753	biological_process	nucleoside phosphate metabolic process
B	0016787	molecular_function	hydrolase activity
B	0016818	molecular_function	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
B	0019693	biological_process	ribose phosphate metabolic process
B	0042803	molecular_function	protein homodimerization activity
B	0046872	molecular_function	metal ion binding
B	0052751	molecular_function	GDP-mannose hydrolase activity
C	0000287	molecular_function	magnesium ion binding
C	0003824	molecular_function	catalytic activity
C	0005829	cellular_component	cytosol
C	0006753	biological_process	nucleoside phosphate metabolic process
C	0016787	molecular_function	hydrolase activity
C	0016818	molecular_function	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
C	0019693	biological_process	ribose phosphate metabolic process
C	0042803	molecular_function	protein homodimerization activity
C	0046872	molecular_function	metal ion binding
C	0052751	molecular_function	GDP-mannose hydrolase activity
D	0000287	molecular_function	magnesium ion binding
D	0003824	molecular_function	catalytic activity
D	0005829	cellular_component	cytosol
D	0006753	biological_process	nucleoside phosphate metabolic process
D	0016787	molecular_function	hydrolase activity
D	0016818	molecular_function	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
D	0019693	biological_process	ribose phosphate metabolic process
D	0042803	molecular_function	protein homodimerization activity
D	0046872	molecular_function	metal ion binding
D	0052751	molecular_function	GDP-mannose hydrolase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	20
Details	BINDING SITE FOR RESIDUE GDD A 3846

Chain	Residue
A	TYR17
A	ILE129
A	GLU151
A	LYS176
A	MG202
A	HOH206
A	HOH210
B	LYS38
B	ARG39
B	GLU40
B	SER121
A	GLY47
B	PRO122
A	ALA48
A	ARG67
A	ALA85
A	GLY86
A	LEU87
A	GLU104
A	GLU127

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG A 202

Chain	Residue
A	ALA85
A	GLU104
A	HOH206
A	GDD3846

---

site_id	AC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE NA A 204

Chain	Residue
A	THR19

---

site_id	AC4
Number of Residues	24
Details	BINDING SITE FOR RESIDUE GDD B 3846

Chain	Residue
A	LYS38
A	ARG39
A	GLU40
A	SER121
A	PRO122
A	GLY123
A	HOH199
B	TYR17
B	PHE18
B	ARG44
B	GLY47
B	ARG67
B	ALA85
B	GLY86
B	LEU87
B	GLU104
B	GLU127
B	ILE129
B	GLU151
B	LYS176
B	HOH196
B	MG202
B	HOH204
B	HOH219

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG B 202

Chain	Residue
B	ALA85
B	GLU104
B	HOH218
B	HOH219
B	GDD3846

---

site_id	AC6
Number of Residues	24
Details	BINDING SITE FOR RESIDUE GDD C 3846

Chain	Residue
C	TYR17
C	PHE18
C	ARG44
C	GLY47
C	ALA48
C	ARG67
C	ALA85
C	GLY86
C	LEU87
C	GLU104
C	GLU127
C	ILE129
C	LYS176
C	MG202
C	HOH209
C	HOH215
C	HOH217
C	NA230
D	LYS38
D	ARG39
D	GLU40
D	SER121
D	PRO122
D	GLY123

---

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG C 202

Chain	Residue
C	ALA85
C	GLU104
C	HOH215
C	HOH217
C	GDD3846

---

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NA C 230

Chain	Residue
C	ARG67
C	GLU82
C	SER83
C	ALA85
C	HOH217
C	GDD3846

---

site_id	AC9
Number of Residues	19
Details	BINDING SITE FOR RESIDUE GDD D 3846

Chain	Residue
D	LEU87
D	GLU104
D	GLU127
D	ILE129
D	ASP150
D	GLU151
D	LYS176
D	MG202
D	HOH223
C	LYS38
C	ARG39
C	GLU40
C	SER121
C	PRO122
D	TYR17
D	ARG44
D	ARG67
D	ALA85
D	GLY86

---

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG D 202

Chain	Residue
D	ALA85
D	GLU104
D	HOH223
D	GDD3846

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	108
Details	Motif: {"description":"Nudix box","evidences":[{"source":"PROSITE-ProRule","id":"PRU00794","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	25
Details	Binding site: {"description":"in other chain","evidences":[{"source":"PubMed","id":"21638333","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3O61","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"21638333","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3O61","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"21638333","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3O6Z","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	137
Details	Domain: {"description":"Nudix hydrolase","evidences":[{"source":"PROSITE-ProRule","id":"PRU00794","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---