3NUH

A domain insertion in E. coli GyrB adopts a novel fold that plays a critical role in gyrase function

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
A	0005524	molecular_function	ATP binding
A	0006259	biological_process	DNA metabolic process
A	0006265	biological_process	DNA topological change
B	0003677	molecular_function	DNA binding
B	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
B	0005524	molecular_function	ATP binding
B	0006265	biological_process	DNA topological change

Functional Information from PROSITE/UniProt

site_id	PS00177
Number of Residues	9
Details	TOPOISOMERASE_II DNA topoisomerase II signature. LVEGDSAGG

Chain	Residue	Details
B	LEU422-GLY430

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01898, ECO:0000305|PubMed:12051843, ECO:0000305|PubMed:18642932

Chain	Residue	Details
B	GLU424
B	ASP498
B	ASP500

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site_id	SWS_FT_FI2
Number of Residues	2
Details	SITE: Interaction with DNA => ECO:0000255|HAMAP-Rule:MF_01898

Chain	Residue	Details
B	LYS449
B	ASN452

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	3
Details	M-CSA 745

Chain	Residue	Details
A	ARG32	electrostatic stabiliser
A	HIS78	proton acceptor
A	TYR122	metal ligand, nucleofuge, nucleophile, proton acceptor, proton donor

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