3LI5
Diisopropyl fluorophosphatase (DFPase), E21Q,N120D,N175D,D229N mutant
Functional Information from GO Data
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 315 |
| Chain | Residue |
| A | ASP232 |
| A | LEU273 |
| A | HIS274 |
| A | HOH317 |
| A | HOH324 |
| A | HOH449 |
| site_id | AC2 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE MES A 411 |
| Chain | Residue |
| A | ASN67 |
| A | GLY68 |
| A | TYR69 |
| A | ARG264 |
| A | PHE311 |
| A | HOH366 |
| A | HOH549 |
| A | GLU7 |
| A | LYS43 |
| A | PRO44 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MES A 412 |
| Chain | Residue |
| A | GLU65 |
| A | THR102 |
| A | PHE103 |
| A | GLN258 |
| A | HOH384 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton acceptor => ECO:0000269|PubMed:15966726 |
| Chain | Residue | Details |
| A | HIS287 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000305 |
| Chain | Residue | Details |
| A | GLN21 | |
| A | ASP120 | |
| A | ASP175 | |
| A | ASN229 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 3 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11435114, ECO:0000269|PubMed:14501113 |
| Chain | Residue | Details |
| A | ASP232 | |
| A | LEU273 | |
| A | HIS274 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 686 |
| Chain | Residue | Details |
| A | GLN21 | increase nucleophilicity, metal ligand, proton acceptor |
| A | GLU37 | electrostatic stabiliser, increase basicity |
| A | ASP120 | metal ligand |
| A | ASP175 | metal ligand |
| A | ASN229 | covalently attached, electrofuge, electrophile, increase nucleophilicity, metal ligand, nucleophile, proton acceptor |
| A | HIS287 | increase nucleophilicity, proton acceptor |
