Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3L4G

Crystal structure of Homo Sapiens cytoplasmic Phenylalanyl-tRNA synthetase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000049	molecular_function	tRNA binding
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0003723	molecular_function	RNA binding
A	0004812	molecular_function	aminoacyl-tRNA ligase activity
A	0004826	molecular_function	phenylalanine-tRNA ligase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006412	biological_process	translation
A	0006432	biological_process	phenylalanyl-tRNA aminoacylation
A	0009328	cellular_component	phenylalanine-tRNA ligase complex
A	0016020	cellular_component	membrane
A	0016874	molecular_function	ligase activity
A	0043039	biological_process	tRNA aminoacylation
A	0046872	molecular_function	metal ion binding
A	0051290	biological_process	protein heterotetramerization
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0003723	molecular_function	RNA binding
B	0004812	molecular_function	aminoacyl-tRNA ligase activity
B	0004826	molecular_function	phenylalanine-tRNA ligase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006412	biological_process	translation
B	0006432	biological_process	phenylalanyl-tRNA aminoacylation
B	0009328	cellular_component	phenylalanine-tRNA ligase complex
B	0016020	cellular_component	membrane
B	0016874	molecular_function	ligase activity
B	0046872	molecular_function	metal ion binding
B	0051290	biological_process	protein heterotetramerization
C	0000049	molecular_function	tRNA binding
C	0000166	molecular_function	nucleotide binding
C	0000287	molecular_function	magnesium ion binding
C	0003723	molecular_function	RNA binding
C	0004812	molecular_function	aminoacyl-tRNA ligase activity
C	0004826	molecular_function	phenylalanine-tRNA ligase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006412	biological_process	translation
C	0006432	biological_process	phenylalanyl-tRNA aminoacylation
C	0009328	cellular_component	phenylalanine-tRNA ligase complex
C	0016020	cellular_component	membrane
C	0016874	molecular_function	ligase activity
C	0043039	biological_process	tRNA aminoacylation
C	0046872	molecular_function	metal ion binding
C	0051290	biological_process	protein heterotetramerization
D	0000166	molecular_function	nucleotide binding
D	0000287	molecular_function	magnesium ion binding
D	0003723	molecular_function	RNA binding
D	0004812	molecular_function	aminoacyl-tRNA ligase activity
D	0004826	molecular_function	phenylalanine-tRNA ligase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006412	biological_process	translation
D	0006432	biological_process	phenylalanyl-tRNA aminoacylation
D	0009328	cellular_component	phenylalanine-tRNA ligase complex
D	0016020	cellular_component	membrane
D	0016874	molecular_function	ligase activity
D	0046872	molecular_function	metal ion binding
D	0051290	biological_process	protein heterotetramerization
E	0000049	molecular_function	tRNA binding
E	0000166	molecular_function	nucleotide binding
E	0000287	molecular_function	magnesium ion binding
E	0003723	molecular_function	RNA binding
E	0004812	molecular_function	aminoacyl-tRNA ligase activity
E	0004826	molecular_function	phenylalanine-tRNA ligase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006412	biological_process	translation
E	0006432	biological_process	phenylalanyl-tRNA aminoacylation
E	0009328	cellular_component	phenylalanine-tRNA ligase complex
E	0016020	cellular_component	membrane
E	0016874	molecular_function	ligase activity
E	0043039	biological_process	tRNA aminoacylation
E	0046872	molecular_function	metal ion binding
E	0051290	biological_process	protein heterotetramerization
F	0000166	molecular_function	nucleotide binding
F	0000287	molecular_function	magnesium ion binding
F	0003723	molecular_function	RNA binding
F	0004812	molecular_function	aminoacyl-tRNA ligase activity
F	0004826	molecular_function	phenylalanine-tRNA ligase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006412	biological_process	translation
F	0006432	biological_process	phenylalanyl-tRNA aminoacylation
F	0009328	cellular_component	phenylalanine-tRNA ligase complex
F	0016020	cellular_component	membrane
F	0016874	molecular_function	ligase activity
F	0046872	molecular_function	metal ion binding
F	0051290	biological_process	protein heterotetramerization
G	0000049	molecular_function	tRNA binding
G	0000166	molecular_function	nucleotide binding
G	0000287	molecular_function	magnesium ion binding
G	0003723	molecular_function	RNA binding
G	0004812	molecular_function	aminoacyl-tRNA ligase activity
G	0004826	molecular_function	phenylalanine-tRNA ligase activity
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006412	biological_process	translation
G	0006432	biological_process	phenylalanyl-tRNA aminoacylation
G	0009328	cellular_component	phenylalanine-tRNA ligase complex
G	0016020	cellular_component	membrane
G	0016874	molecular_function	ligase activity
G	0043039	biological_process	tRNA aminoacylation
G	0046872	molecular_function	metal ion binding
G	0051290	biological_process	protein heterotetramerization
H	0000166	molecular_function	nucleotide binding
H	0000287	molecular_function	magnesium ion binding
H	0003723	molecular_function	RNA binding
H	0004812	molecular_function	aminoacyl-tRNA ligase activity
H	0004826	molecular_function	phenylalanine-tRNA ligase activity
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005654	cellular_component	nucleoplasm
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006412	biological_process	translation
H	0006432	biological_process	phenylalanyl-tRNA aminoacylation
H	0009328	cellular_component	phenylalanine-tRNA ligase complex
H	0016020	cellular_component	membrane
H	0016874	molecular_function	ligase activity
H	0046872	molecular_function	metal ion binding
H	0051290	biological_process	protein heterotetramerization
I	0000049	molecular_function	tRNA binding
I	0000166	molecular_function	nucleotide binding
I	0000287	molecular_function	magnesium ion binding
I	0003723	molecular_function	RNA binding
I	0004812	molecular_function	aminoacyl-tRNA ligase activity
I	0004826	molecular_function	phenylalanine-tRNA ligase activity
I	0005515	molecular_function	protein binding
I	0005524	molecular_function	ATP binding
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006412	biological_process	translation
I	0006432	biological_process	phenylalanyl-tRNA aminoacylation
I	0009328	cellular_component	phenylalanine-tRNA ligase complex
I	0016020	cellular_component	membrane
I	0016874	molecular_function	ligase activity
I	0043039	biological_process	tRNA aminoacylation
I	0046872	molecular_function	metal ion binding
I	0051290	biological_process	protein heterotetramerization
J	0000166	molecular_function	nucleotide binding
J	0000287	molecular_function	magnesium ion binding
J	0003723	molecular_function	RNA binding
J	0004812	molecular_function	aminoacyl-tRNA ligase activity
J	0004826	molecular_function	phenylalanine-tRNA ligase activity
J	0005515	molecular_function	protein binding
J	0005524	molecular_function	ATP binding
J	0005654	cellular_component	nucleoplasm
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0006412	biological_process	translation
J	0006432	biological_process	phenylalanyl-tRNA aminoacylation
J	0009328	cellular_component	phenylalanine-tRNA ligase complex
J	0016020	cellular_component	membrane
J	0016874	molecular_function	ligase activity
J	0046872	molecular_function	metal ion binding
J	0051290	biological_process	protein heterotetramerization
K	0000049	molecular_function	tRNA binding
K	0000166	molecular_function	nucleotide binding
K	0000287	molecular_function	magnesium ion binding
K	0003723	molecular_function	RNA binding
K	0004812	molecular_function	aminoacyl-tRNA ligase activity
K	0004826	molecular_function	phenylalanine-tRNA ligase activity
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006412	biological_process	translation
K	0006432	biological_process	phenylalanyl-tRNA aminoacylation
K	0009328	cellular_component	phenylalanine-tRNA ligase complex
K	0016020	cellular_component	membrane
K	0016874	molecular_function	ligase activity
K	0043039	biological_process	tRNA aminoacylation
K	0046872	molecular_function	metal ion binding
K	0051290	biological_process	protein heterotetramerization
L	0000166	molecular_function	nucleotide binding
L	0000287	molecular_function	magnesium ion binding
L	0003723	molecular_function	RNA binding
L	0004812	molecular_function	aminoacyl-tRNA ligase activity
L	0004826	molecular_function	phenylalanine-tRNA ligase activity
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0005654	cellular_component	nucleoplasm
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0006412	biological_process	translation
L	0006432	biological_process	phenylalanyl-tRNA aminoacylation
L	0009328	cellular_component	phenylalanine-tRNA ligase complex
L	0016020	cellular_component	membrane
L	0016874	molecular_function	ligase activity
L	0046872	molecular_function	metal ion binding
L	0051290	biological_process	protein heterotetramerization
M	0000049	molecular_function	tRNA binding
M	0000166	molecular_function	nucleotide binding
M	0000287	molecular_function	magnesium ion binding
M	0003723	molecular_function	RNA binding
M	0004812	molecular_function	aminoacyl-tRNA ligase activity
M	0004826	molecular_function	phenylalanine-tRNA ligase activity
M	0005515	molecular_function	protein binding
M	0005524	molecular_function	ATP binding
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0006412	biological_process	translation
M	0006432	biological_process	phenylalanyl-tRNA aminoacylation
M	0009328	cellular_component	phenylalanine-tRNA ligase complex
M	0016020	cellular_component	membrane
M	0016874	molecular_function	ligase activity
M	0043039	biological_process	tRNA aminoacylation
M	0046872	molecular_function	metal ion binding
M	0051290	biological_process	protein heterotetramerization
N	0000166	molecular_function	nucleotide binding
N	0000287	molecular_function	magnesium ion binding
N	0003723	molecular_function	RNA binding
N	0004812	molecular_function	aminoacyl-tRNA ligase activity
N	0004826	molecular_function	phenylalanine-tRNA ligase activity
N	0005515	molecular_function	protein binding
N	0005524	molecular_function	ATP binding
N	0005654	cellular_component	nucleoplasm
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0006412	biological_process	translation
N	0006432	biological_process	phenylalanyl-tRNA aminoacylation
N	0009328	cellular_component	phenylalanine-tRNA ligase complex
N	0016020	cellular_component	membrane
N	0016874	molecular_function	ligase activity
N	0046872	molecular_function	metal ion binding
N	0051290	biological_process	protein heterotetramerization
O	0000049	molecular_function	tRNA binding
O	0000166	molecular_function	nucleotide binding
O	0000287	molecular_function	magnesium ion binding
O	0003723	molecular_function	RNA binding
O	0004812	molecular_function	aminoacyl-tRNA ligase activity
O	0004826	molecular_function	phenylalanine-tRNA ligase activity
O	0005515	molecular_function	protein binding
O	0005524	molecular_function	ATP binding
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0006412	biological_process	translation
O	0006432	biological_process	phenylalanyl-tRNA aminoacylation
O	0009328	cellular_component	phenylalanine-tRNA ligase complex
O	0016020	cellular_component	membrane
O	0016874	molecular_function	ligase activity
O	0043039	biological_process	tRNA aminoacylation
O	0046872	molecular_function	metal ion binding
O	0051290	biological_process	protein heterotetramerization
P	0000166	molecular_function	nucleotide binding
P	0000287	molecular_function	magnesium ion binding
P	0003723	molecular_function	RNA binding
P	0004812	molecular_function	aminoacyl-tRNA ligase activity
P	0004826	molecular_function	phenylalanine-tRNA ligase activity
P	0005515	molecular_function	protein binding
P	0005524	molecular_function	ATP binding
P	0005654	cellular_component	nucleoplasm
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0006412	biological_process	translation
P	0006432	biological_process	phenylalanyl-tRNA aminoacylation
P	0009328	cellular_component	phenylalanine-tRNA ligase complex
P	0016020	cellular_component	membrane
P	0016874	molecular_function	ligase activity
P	0046872	molecular_function	metal ion binding
P	0051290	biological_process	protein heterotetramerization

Functional Information from PDB Data

site_id	AC1
Number of Residues	12
Details	BINDING SITE FOR RESIDUE PHE A 509

Chain	Residue
A	THR329
A	PHE438
A	TRP457
A	GLY458
A	GLN372
A	GLU374
A	ASN410
A	TYR412
A	THR413
A	ASN434
A	SER435
A	GLY436

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE PHE C 509

Chain	Residue
C	THR329
C	GLN372
C	GLU374
C	ASN434
C	SER435
C	GLY436
C	GLY458

site_id	AC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PHE E 509

Chain	Residue
E	GLN372
E	GLU374
E	ASN410
E	TYR412
E	ASN434
E	ALA456
E	TRP457
E	GLY458
E	LEU459

site_id	AC4
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PHE G 509

Chain	Residue
G	THR329
G	GLN372
G	GLU374
G	ASN410
G	ASN434
G	SER435
G	GLY436
G	ALA456
G	TRP457
G	GLY458

site_id	AC5
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PHE I 509

Chain	Residue
I	HIS327
I	THR329
I	GLN372
I	GLU374
I	ASN410
I	ASN434
I	SER435
I	GLY436
I	ALA456

site_id	AC6
Number of Residues	12
Details	BINDING SITE FOR RESIDUE PHE K 509

Chain	Residue
K	THR329
K	GLN372
K	GLU374
K	ASN410
K	TYR412
K	THR413
K	ASN434
K	SER435
K	GLY436
K	PHE438
K	TRP457
K	GLY458

site_id	AC7
Number of Residues	11
Details	BINDING SITE FOR RESIDUE PHE M 509

Chain	Residue
M	THR329
M	GLN372
M	GLU374
M	TYR412
M	THR413
M	ASN434
M	SER435
M	GLY436
M	PHE438
M	TRP457
M	GLY458

site_id	AC8
Number of Residues	9
Details	BINDING SITE FOR RESIDUE PHE O 509

Chain	Residue
O	HIS327
O	THR329
O	GLN372
O	GLU374
O	ASN434
O	SER435
O	GLY436
O	PHE438
O	ALA456

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	40
Details	Binding site: {"evidences":[{"source":"PubMed","id":"20223217","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3L4G","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"A5K9S0","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	8
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18691976","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"Q8C0C7","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	8
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	2
Details	Modified residue: {"description":"N-acetylalanine","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"OCT-2004","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V."]}},{"source":"PubMed","id":"19413330","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22223895","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"25944712","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	616
Details	Domain: {"description":"B5","evidences":[{"source":"PROSITE-ProRule","id":"PRU00816","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	32
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00816","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)