3KJM

Leu492Ala mutant of Maize cytokinin oxidase/dehydrogenase complexed with phenylurea inhibitor CPPU

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0005576	cellular_component	extracellular region
A	0009690	biological_process	cytokinin metabolic process
A	0016491	molecular_function	oxidoreductase activity
A	0019139	molecular_function	cytokinin dehydrogenase activity
A	0050660	molecular_function	flavin adenine dinucleotide binding
A	0071949	molecular_function	FAD binding

Functional Information from PROSITE/UniProt

site_id	PS00862
Number of Residues	37
Details	OX2_COVAL_FAD Oxygen oxidoreductases covalent FAD-binding site. PaavlypsStaDLvalLsaanstpgwpytIafrGRGH

Chain	Residue	Details
A	PRO69-HIS105

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	13
Details	BINDING: BINDING => ECO:0000269|PubMed:15321719, ECO:0007744|PDB:1W1O, ECO:0007744|PDB:1W1Q

Chain	Residue	Details
A	PHE100
A	ILE235
A	TYR491
A	SER527
A	GLN530
A	GLY102
A	ARG103
A	GLY104
A	SER106
A	GLN110
A	THR174
A	SER180
A	ILE184

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site_id	SWS_FT_FI2
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269|PubMed:15321719, ECO:0007744|PDB:1W1R

Chain	Residue	Details
A	ASP169
A	GLU381
A	SER456

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site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Pros-8alpha-FAD histidine => ECO:0000269|PubMed:15321719, ECO:0007744|PDB:1W1O, ECO:0007744|PDB:1W1Q

Chain	Residue	Details
A	HIS105

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site_id	SWS_FT_FI4
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
A	ASN52
A	ASN434

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site_id	SWS_FT_FI5
Number of Residues	4
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:15321719, ECO:0007744|PDB:1W1O, ECO:0007744|PDB:1W1Q

Chain	Residue	Details
A	ASN63
A	ASN89
A	ASN134
A	ASN294

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site_id	SWS_FT_FI6
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:18571199, ECO:0007744|PDB:3BW7

Chain	Residue	Details
A	ASN323
A	ASN338

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	3
Details	M-CSA 115

Chain	Residue	Details
A	HIS105	alter redox potential, covalently attached
A	ASP169	electrostatic stabiliser, hydrogen bond acceptor
A	GLU288	activator, hydrogen bond donor

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