Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3IE2

Crystal Structure of H400V mutant TTHA0252 from Thermus thermophilus HB8

Replaces: 2YVD

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004521	molecular_function	RNA endonuclease activity
A	0005737	cellular_component	cytoplasm
A	0006364	biological_process	rRNA processing
A	0016787	molecular_function	hydrolase activity
A	0046872	molecular_function	metal ion binding
B	0003723	molecular_function	RNA binding
B	0004518	molecular_function	nuclease activity
B	0004519	molecular_function	endonuclease activity
B	0004521	molecular_function	RNA endonuclease activity
B	0005737	cellular_component	cytoplasm
B	0006364	biological_process	rRNA processing
B	0016787	molecular_function	hydrolase activity
B	0046872	molecular_function	metal ion binding
C	0003723	molecular_function	RNA binding
C	0004518	molecular_function	nuclease activity
C	0004519	molecular_function	endonuclease activity
C	0004521	molecular_function	RNA endonuclease activity
C	0005737	cellular_component	cytoplasm
C	0006364	biological_process	rRNA processing
C	0016787	molecular_function	hydrolase activity
C	0046872	molecular_function	metal ion binding
D	0003723	molecular_function	RNA binding
D	0004518	molecular_function	nuclease activity
D	0004519	molecular_function	endonuclease activity
D	0004521	molecular_function	RNA endonuclease activity
D	0005737	cellular_component	cytoplasm
D	0006364	biological_process	rRNA processing
D	0016787	molecular_function	hydrolase activity
D	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 432

Chain	Residue
A	TYR189
A	ARG227
A	GLY340
A	TYR341
A	SER378
A	GLY379

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 433

Chain	Residue
A	MET1
A	ARG2
A	LEU20

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 434

Chain	Residue
A	GLU292
A	HIS293
A	THR294
A	BTB456

site_id	AC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 435

Chain	Residue
A	ARG2
A	ARG25

site_id	AC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 A 436

Chain	Residue
A	SO4453

site_id	AC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 437

Chain	Residue
A	GLU199
A	ARG202

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 438

Chain	Residue
A	PRO243
A	ARG244
B	ARG302

site_id	AC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 439

Chain	Residue
A	ARG284
A	HOH460

site_id	AC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 440

Chain	Residue
A	LEU407
A	GLY410
A	LYS411
A	ALA414
B	GLY410
B	LYS411
B	ALA414

site_id	BC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 441

Chain	Residue
A	ARG24
A	LEU129
A	GLY130

site_id	BC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 442

Chain	Residue
A	ARG2
A	GLY426
A	GLY428

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 443

Chain	Residue
A	THR222
A	PHE223
A	ALA224
A	SER313
A	GLY314

site_id	BC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 444

Chain	Residue
A	HIS238
A	HIS240
A	ARG241
B	ARG368

site_id	BC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 A 445

Chain	Residue
A	ARG354

site_id	BC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 A 446

Chain	Residue
A	ARG333

site_id	BC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 447

Chain	Residue
A	PRO366
A	ARG368

site_id	BC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 448

Chain	Residue
A	LYS298
A	ASN301
A	HIS323
A	LYS326
A	HIS327

site_id	BC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 449

Chain	Residue
A	PRO172
A	ASP173
A	SER269
A	GLU270
D	ARG84

site_id	CC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 A 450

Chain	Residue
A	HIS293

site_id	CC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 A 451

Chain	Residue
A	HIS323

site_id	CC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 452

Chain	Residue
A	ARG73
C	ARG10

site_id	CC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 453

Chain	Residue
A	LEU88
A	ARG263
A	SO4436

site_id	CC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 A 454

Chain	Residue
A	ARG368

site_id	CC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 455

Chain	Residue
A	LYS99
A	PRO252
A	ARG256
A	BTB456

site_id	CC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE BTB A 456

Chain	Residue
A	PRO252
A	THR294
A	GLY319
A	ARG320
A	SO4434
A	SO4455

site_id	CC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 457

Chain	Residue
A	HIS59
A	HIS61
A	HIS141
A	ASP162

site_id	CC9
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 B 432

Chain	Residue
B	ARG354

site_id	DC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 B 433

Chain	Residue
B	ARG359
B	GLY362

site_id	DC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 434

Chain	Residue
B	ARG24
B	LEU129
B	GLY130

site_id	DC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 B 435

Chain	Residue
B	ARG24

site_id	DC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 436

Chain	Residue
B	ARG10
B	ARG424
B	PHE425

site_id	DC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 437

Chain	Residue
B	PHE223
B	ALA224
B	SER313
B	GLY314

site_id	DC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 438

Chain	Residue
B	PRO172
B	ASP173
B	SER269
B	GLU270

site_id	DC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 B 439

Chain	Residue
A	ARG244
B	LYS298
B	HIS323
B	LYS326
B	HIS327

site_id	DC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 440

Chain	Residue
B	ARG302
B	PRO304
B	ARG333

site_id	DC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 441

Chain	Residue
B	ARG192
B	PRO193
B	ARG195

site_id	EC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 B 442

Chain	Residue
B	PRO243
B	ARG244

site_id	EC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 443

Chain	Residue
B	ARG84
B	LEU88
B	ARG263

site_id	EC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 B 444

Chain	Residue
B	ARG73

site_id	EC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 445

Chain	Residue
B	HIS293
B	THR294
B	ARG320

site_id	EC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 B 446

Chain	Residue
B	ARG2
B	GLY428

site_id	EC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 447

Chain	Residue
B	ARG25
B	PHE47
B	ASP48

site_id	EC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 448

Chain	Residue
B	HIS61
B	LEU62
B	ASP63

site_id	EC8
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 B 449

Chain	Residue
B	ARG368

site_id	EC9
Number of Residues	8
Details	BINDING SITE FOR RESIDUE SO4 B 450

Chain	Residue
B	TYR189
B	PHE223
B	ARG227
B	GLY340
B	TYR341
B	SER378
B	GLY379
B	HIS380

site_id	FC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 451

Chain	Residue
B	HIS59
B	HIS61
B	HIS141
B	ASP162

site_id	FC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 C 432

Chain	Residue
C	TYR189
C	ARG227
C	GLY340
C	TYR341
C	SER378
C	GLY379
C	HIS380

site_id	FC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 C 433

Chain	Residue
C	PRO172
C	ASP173
C	SER269
C	GLU270

site_id	FC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 C 434

Chain	Residue
C	LYS298
C	ASN301
C	HIS323
C	HIS327

site_id	FC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C 435

Chain	Residue
C	ARG84
C	ARG263
C	HOH455

site_id	FC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 C 436

Chain	Residue
C	ARG155

site_id	FC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 C 437

Chain	Residue
C	GLU292
C	HIS293

site_id	FC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 C 438

Chain	Residue
C	ARG25
C	GLY46
C	PHE47
C	ASP48

site_id	FC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C 439

Chain	Residue
C	ARG24
C	LEU129
C	GLY130

site_id	GC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 C 440

Chain	Residue
C	MET1
C	ARG2

site_id	GC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C 441

Chain	Residue
C	HIS61
C	LEU62
C	VAL225

site_id	GC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 C 442

Chain	Residue
C	ARG2
C	GLY428

site_id	GC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 C 443

Chain	Residue
C	ARG24

site_id	GC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 C 444

Chain	Residue
B	ARG266
B	GLN273
B	LEU277
C	VAL265
C	ARG266
C	GLN273
C	LEU277

site_id	GC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 C 445

Chain	Residue
C	ARG10
C	ARG424

site_id	GC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN C 446

Chain	Residue
C	HIS59
C	HIS61
C	HIS141
C	ASP162
C	HOH447

site_id	GC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 D 432

Chain	Residue
D	TYR189
D	ARG227
D	GLY340
D	TYR341
D	SER378
D	GLY379
D	HIS380

site_id	GC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 D 433

Chain	Residue
D	ARG24
D	ARG128
D	LEU129
D	GLY130

site_id	HC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 D 434

Chain	Residue
D	PRO252
D	MET253
D	ARG256

site_id	HC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 D 435

Chain	Residue
D	PRO172
D	ASP173
D	SER269
D	GLU270

site_id	HC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 D 436

Chain	Residue
D	ARG2
D	LYS50
D	GLY428

site_id	HC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 D 437

Chain	Residue
D	ARG77
D	HIS117

site_id	HC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 D 438

Chain	Residue
D	ARG24

site_id	HC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 D 439

Chain	Residue
D	ARG263

site_id	HC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 440

Chain	Residue
D	HIS59
D	HIS61
D	HIS141
D	ASP162

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	28
Details	Binding site: {}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1qh5

Chain	Residue	Details
A	ASP63

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1qh5

Chain	Residue	Details
B	ASP63

site_id	CSA3
Number of Residues	1
Details	Annotated By Reference To The Literature 1qh5

Chain	Residue	Details
C	ASP63

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1qh5

Chain	Residue	Details
D	ASP63

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)