Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3IE1

Crystal structure of H380A mutant TTHA0252 from Thermus thermophilus HB8 complexed with RNA

Replaces: 2ZDE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003723	molecular_function	RNA binding
A	0004519	molecular_function	endonuclease activity
A	0004521	molecular_function	RNA endonuclease activity
A	0005737	cellular_component	cytoplasm
A	0006364	biological_process	rRNA processing
A	0046872	molecular_function	metal ion binding
B	0003723	molecular_function	RNA binding
B	0004519	molecular_function	endonuclease activity
B	0004521	molecular_function	RNA endonuclease activity
B	0005737	cellular_component	cytoplasm
B	0006364	biological_process	rRNA processing
B	0046872	molecular_function	metal ion binding
C	0003723	molecular_function	RNA binding
C	0004519	molecular_function	endonuclease activity
C	0004521	molecular_function	RNA endonuclease activity
C	0005737	cellular_component	cytoplasm
C	0006364	biological_process	rRNA processing
C	0046872	molecular_function	metal ion binding
D	0003723	molecular_function	RNA binding
D	0004519	molecular_function	endonuclease activity
D	0004521	molecular_function	RNA endonuclease activity
D	0005737	cellular_component	cytoplasm
D	0006364	biological_process	rRNA processing
D	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 432

Chain	Residue
A	ARG192
A	ARG195
A	GLY375
A	GLY376
A	HOH458

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 433

Chain	Residue
A	HIS238
A	HIS240
A	ARG241
B	ARG368

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 434

Chain	Residue
A	ARG24
A	ARG128
A	LEU129
A	GLY130

site_id	AC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 A 435

Chain	Residue
A	FLC450

site_id	AC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 A 436

Chain	Residue
A	ARG333

site_id	AC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 437

Chain	Residue
A	MET1
A	ARG2

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 438

Chain	Residue
A	PRO172
A	ASP173
A	SER269
A	GLU270

site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 439

Chain	Residue
A	SER330
A	PRO366
A	ARG368

site_id	AC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 440

Chain	Residue
A	ARG359
A	GLY362

site_id	BC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 A 441

Chain	Residue
A	ARG368

site_id	BC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 442

Chain	Residue
A	HIS293
B	ARG284

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 443

Chain	Residue
A	LYS298
A	ASN301
A	HIS323
A	LYS326
A	HIS327

site_id	BC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 444

Chain	Residue
A	HIS293
A	THR294
A	GLU295
B	GLY287

site_id	BC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 445

Chain	Residue
A	ASN281
A	ARG284
A	FLC450
A	HOH461

site_id	BC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 446

Chain	Residue
A	ARG2
A	GLU427
A	GLY428

site_id	BC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 447

Chain	Residue
A	TYR189
A	ARG227
A	GLY340
A	TYR341
A	SER378
A	GLY379

site_id	BC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 448

Chain	Residue
A	ARG284
A	VAL290
A	FLC450
B	GLU292

site_id	BC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 449

Chain	Residue
A	ARG84
A	LEU88
A	ARG263

site_id	CC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE FLC A 450

Chain	Residue
A	SER259
A	PRO262
A	ARG284
A	SO4435
A	SO4445
A	SO4448

site_id	CC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FLC A 451

Chain	Residue
A	LEU407
A	LYS411
A	ALA414
B	LEU407
B	LYS411

site_id	CC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN A 452

Chain	Residue
A	ASP63
A	HIS64
A	ASP162
A	HIS400
A	ZN453
E	U1

site_id	CC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN A 453

Chain	Residue
A	HIS59
A	HIS61
A	HIS141
A	ASP162
A	ZN452
E	U1

site_id	CC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 B 432

Chain	Residue
B	LEU387

site_id	CC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 B 433

Chain	Residue
B	ARG359
B	GLY362

site_id	CC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 B 434

Chain	Residue
B	PRO243
B	ARG244

site_id	CC8
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 B 435

Chain	Residue
B	ARG24

site_id	CC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 436

Chain	Residue
B	ARG284
B	VAL290
B	GLU292

site_id	DC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 B 437

Chain	Residue
A	LEU415
B	ARG424

site_id	DC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 B 438

Chain	Residue
A	PRO243
A	ARG244

site_id	DC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 B 439

Chain	Residue
B	ARG354

site_id	DC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 440

Chain	Residue
B	PRO172
B	ASP173
B	SER269
B	GLU270

site_id	DC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 441

Chain	Residue
B	ARG24
B	LEU129
B	GLY130

site_id	DC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 B 442

Chain	Residue
B	GLU292
B	HIS293
B	THR294
B	ARG320
F	U4

site_id	DC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 B 443

Chain	Residue
B	ARG84
B	ARG263

site_id	DC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 B 444

Chain	Residue
A	ARG244
B	LYS298
B	HIS323
B	LYS326
B	HIS327

site_id	DC9
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 B 445

Chain	Residue
B	ARG368

site_id	EC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 446

Chain	Residue
B	ARG2
B	GLY426
B	GLY428

site_id	EC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 447

Chain	Residue
B	ARG25
B	PHE47
B	ASP48
B	GLU51

site_id	EC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 B 448

Chain	Residue
B	TYR189
B	ARG227
B	GLY340
B	TYR341
B	SER378
B	GLY379

site_id	EC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN B 449

Chain	Residue
B	ASP63
B	HIS64
B	ASP162
B	HIS400
B	ZN450
F	U1

site_id	EC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN B 450

Chain	Residue
B	HIS59
B	HIS61
B	HIS141
B	ASP162
B	ZN449
F	U1

site_id	EC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 C 432

Chain	Residue
C	TYR189
C	ARG227
C	TYR341
C	SER378
C	GLY379
C	ALA380

site_id	EC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 C 433

Chain	Residue
C	PRO172
C	ASP173
C	SER269
C	GLU270

site_id	EC8
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 C 434

Chain	Residue
C	ARG25

site_id	EC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 C 435

Chain	Residue
C	ARG24
C	LYS50

site_id	FC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C 436

Chain	Residue
C	SER251
C	PRO252
C	MET253

site_id	FC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 C 437

Chain	Residue
A	ARG73
C	ARG424

site_id	FC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 C 438

Chain	Residue
C	ARG84
C	ARG263

site_id	FC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 C 439

Chain	Residue
C	ARG24
C	ARG128
C	LEU129
C	GLY130

site_id	FC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 C 440

Chain	Residue
C	ARG2
C	GLY428

site_id	FC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 C 441

Chain	Residue
C	ARG25
C	PHE47
C	ASP48

site_id	FC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN C 442

Chain	Residue
C	ASP63
C	HIS64
C	ASP162
C	HIS400
C	ZN443
G	U1

site_id	FC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN C 443

Chain	Residue
C	HIS59
C	HIS61
C	HIS141
C	ASP162
C	ZN442
G	U1

site_id	FC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 D 432

Chain	Residue
D	GLY35
D	LYS36
D	GLU38

site_id	GC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 D 433

Chain	Residue
D	ARG24

site_id	GC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 D 434

Chain	Residue
D	ARG84
D	ARG263

site_id	GC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 D 435

Chain	Residue
D	ARG155

site_id	GC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 D 436

Chain	Residue
D	PRO172
D	ASP173
D	SER269
D	GLU270

site_id	GC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 D 437

Chain	Residue
D	ARG2
D	GLY428

site_id	GC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 D 438

Chain	Residue
D	ARG24
D	ARG128
D	LEU129
D	GLY130

site_id	GC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 D 439

Chain	Residue
D	TYR189
D	ARG227
D	GLY340
D	TYR341
D	SER378
D	GLY379

site_id	GC8
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 D 440

Chain	Residue
D	MET1

site_id	GC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN D 441

Chain	Residue
D	ASP63
D	HIS64
D	ASP162
D	HIS400
D	ZN442
H	U1

site_id	HC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE ZN D 442

Chain	Residue
D	HIS59
D	HIS61
D	HIS141
D	ASP162
D	ZN441
H	U1

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	28
Details	BINDING:

Chain	Residue	Details
A	HIS59
B	ASP63
B	HIS64
B	HIS141
B	ASP162
B	HIS400
C	HIS59
C	HIS61
C	ASP63
C	HIS64
C	HIS141
A	HIS61
C	ASP162
C	HIS400
D	HIS59
D	HIS61
D	ASP63
D	HIS64
D	HIS141
D	ASP162
D	HIS400
A	ASP63
A	HIS64
A	HIS141
A	ASP162
A	HIS400
B	HIS59
B	HIS61

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1qh5

Chain	Residue	Details
A	ASP63

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1qh5

Chain	Residue	Details
B	ASP63

site_id	CSA3
Number of Residues	1
Details	Annotated By Reference To The Literature 1qh5

Chain	Residue	Details
C	ASP63

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1qh5

Chain	Residue	Details
D	ASP63

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)