3HO1

Crystal structure of T. thermophilus Argonaute N546 mutant protein complexed with DNA guide strand and 12-nt RNA target strand

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0003723	molecular_function	RNA binding
A	0004520	molecular_function	DNA endonuclease activity
A	0030145	molecular_function	manganese ion binding
A	0044355	biological_process	clearance of foreign intracellular DNA

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG X 22

Chain	Residue
A	VAL685
X	DT1
X	DA3

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	164
Details	Domain: {"description":"Piwi","evidences":[{"source":"PROSITE-ProRule","id":"PRU00150","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	76
Details	Region: {"description":"Linker L1","evidences":[{"source":"PubMed","id":"18754009","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	65
Details	Region: {"description":"Linker L2","evidences":[{"source":"PubMed","id":"18754009","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI4
Number of Residues	125
Details	Region: {"description":"Mid domain","evidences":[{"source":"PubMed","id":"18754009","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	Active site: {"evidences":[{"source":"PubMed","id":"18754009","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"24374628","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"28911094","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI6
Number of Residues	1
Details	Active site: {"evidences":[{"source":"PubMed","id":"24374628","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"28911094","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI7
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24374628","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19812667","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"28911094","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"4KPY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N76","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI8
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24374628","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19812667","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"28911094","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"4KPY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N76","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24374628","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18754009","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19812667","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"28911094","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"4KPY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N76","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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