Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3HJO

Crystal Structure of Glutathione Transferase Pi Y108V Mutant in Complex with the Glutathione Conjugate of Ethacrynic Acid

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0000302	biological_process	response to reactive oxygen species
A	0002674	biological_process	negative regulation of acute inflammatory response
A	0004364	molecular_function	glutathione transferase activity
A	0004602	molecular_function	glutathione peroxidase activity
A	0005504	molecular_function	fatty acid binding
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005615	cellular_component	extracellular space
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0006629	biological_process	lipid metabolic process
A	0006693	biological_process	prostaglandin metabolic process
A	0006749	biological_process	glutathione metabolic process
A	0006805	biological_process	xenobiotic metabolic process
A	0007417	biological_process	central nervous system development
A	0008432	molecular_function	JUN kinase binding
A	0009636	biological_process	response to toxic substance
A	0010804	biological_process	negative regulation of tumor necrosis factor-mediated signaling pathway
A	0014003	biological_process	oligodendrocyte development
A	0015643	molecular_function	toxic substance binding
A	0016740	molecular_function	transferase activity
A	0019901	molecular_function	protein kinase binding
A	0030291	molecular_function	protein serine/threonine kinase inhibitor activity
A	0031100	biological_process	animal organ regeneration
A	0031667	biological_process	response to nutrient levels
A	0031982	cellular_component	vesicle
A	0032355	biological_process	response to estradiol
A	0032691	biological_process	negative regulation of interleukin-1 beta production
A	0032720	biological_process	negative regulation of tumor necrosis factor production
A	0032869	biological_process	cellular response to insulin stimulus
A	0032872	biological_process	regulation of stress-activated MAPK cascade
A	0032873	biological_process	negative regulation of stress-activated MAPK cascade
A	0032930	biological_process	positive regulation of superoxide anion generation
A	0033591	biological_process	response to L-ascorbic acid
A	0034774	cellular_component	secretory granule lumen
A	0035726	biological_process	common myeloid progenitor cell proliferation
A	0035730	molecular_function	S-nitrosoglutathione binding
A	0035731	molecular_function	dinitrosyl-iron complex binding
A	0035732	biological_process	nitric oxide storage
A	0043066	biological_process	negative regulation of apoptotic process
A	0043124	biological_process	negative regulation of canonical NF-kappaB signal transduction
A	0043200	biological_process	response to amino acid
A	0043409	biological_process	negative regulation of MAPK cascade
A	0043651	biological_process	linoleic acid metabolic process
A	0045471	biological_process	response to ethanol
A	0046329	biological_process	negative regulation of JNK cascade
A	0048147	biological_process	negative regulation of fibroblast proliferation
A	0051122	biological_process	hepoxilin biosynthetic process
A	0070026	molecular_function	nitric oxide binding
A	0070062	cellular_component	extracellular exosome
A	0070372	biological_process	regulation of ERK1 and ERK2 cascade
A	0070373	biological_process	negative regulation of ERK1 and ERK2 cascade
A	0070664	biological_process	negative regulation of leukocyte proliferation
A	0071222	biological_process	cellular response to lipopolysaccharide
A	0071364	biological_process	cellular response to epidermal growth factor stimulus
A	0071385	biological_process	cellular response to glucocorticoid stimulus
A	0071460	biological_process	cellular response to cell-matrix adhesion
A	0071638	biological_process	negative regulation of monocyte chemotactic protein-1 production
A	0071672	biological_process	negative regulation of smooth muscle cell chemotaxis
A	0097057	cellular_component	TRAF2-GSTP1 complex
A	0098869	biological_process	cellular oxidant detoxification
A	0110076	biological_process	negative regulation of ferroptosis
A	1901687	biological_process	glutathione derivative biosynthetic process
A	1904706	biological_process	negative regulation of vascular associated smooth muscle cell proliferation
A	1904813	cellular_component	ficolin-1-rich granule lumen
A	2001237	biological_process	negative regulation of extrinsic apoptotic signaling pathway
B	0000122	biological_process	negative regulation of transcription by RNA polymerase II
B	0000302	biological_process	response to reactive oxygen species
B	0002674	biological_process	negative regulation of acute inflammatory response
B	0004364	molecular_function	glutathione transferase activity
B	0004602	molecular_function	glutathione peroxidase activity
B	0005504	molecular_function	fatty acid binding
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005615	cellular_component	extracellular space
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005829	cellular_component	cytosol
B	0006629	biological_process	lipid metabolic process
B	0006693	biological_process	prostaglandin metabolic process
B	0006749	biological_process	glutathione metabolic process
B	0006805	biological_process	xenobiotic metabolic process
B	0007417	biological_process	central nervous system development
B	0008432	molecular_function	JUN kinase binding
B	0009636	biological_process	response to toxic substance
B	0010804	biological_process	negative regulation of tumor necrosis factor-mediated signaling pathway
B	0014003	biological_process	oligodendrocyte development
B	0015643	molecular_function	toxic substance binding
B	0016740	molecular_function	transferase activity
B	0019901	molecular_function	protein kinase binding
B	0030291	molecular_function	protein serine/threonine kinase inhibitor activity
B	0031100	biological_process	animal organ regeneration
B	0031667	biological_process	response to nutrient levels
B	0031982	cellular_component	vesicle
B	0032355	biological_process	response to estradiol
B	0032691	biological_process	negative regulation of interleukin-1 beta production
B	0032720	biological_process	negative regulation of tumor necrosis factor production
B	0032869	biological_process	cellular response to insulin stimulus
B	0032872	biological_process	regulation of stress-activated MAPK cascade
B	0032873	biological_process	negative regulation of stress-activated MAPK cascade
B	0032930	biological_process	positive regulation of superoxide anion generation
B	0033591	biological_process	response to L-ascorbic acid
B	0034774	cellular_component	secretory granule lumen
B	0035726	biological_process	common myeloid progenitor cell proliferation
B	0035730	molecular_function	S-nitrosoglutathione binding
B	0035731	molecular_function	dinitrosyl-iron complex binding
B	0035732	biological_process	nitric oxide storage
B	0043066	biological_process	negative regulation of apoptotic process
B	0043124	biological_process	negative regulation of canonical NF-kappaB signal transduction
B	0043200	biological_process	response to amino acid
B	0043409	biological_process	negative regulation of MAPK cascade
B	0043651	biological_process	linoleic acid metabolic process
B	0045471	biological_process	response to ethanol
B	0046329	biological_process	negative regulation of JNK cascade
B	0048147	biological_process	negative regulation of fibroblast proliferation
B	0051122	biological_process	hepoxilin biosynthetic process
B	0070026	molecular_function	nitric oxide binding
B	0070062	cellular_component	extracellular exosome
B	0070372	biological_process	regulation of ERK1 and ERK2 cascade
B	0070373	biological_process	negative regulation of ERK1 and ERK2 cascade
B	0070664	biological_process	negative regulation of leukocyte proliferation
B	0071222	biological_process	cellular response to lipopolysaccharide
B	0071364	biological_process	cellular response to epidermal growth factor stimulus
B	0071385	biological_process	cellular response to glucocorticoid stimulus
B	0071460	biological_process	cellular response to cell-matrix adhesion
B	0071638	biological_process	negative regulation of monocyte chemotactic protein-1 production
B	0071672	biological_process	negative regulation of smooth muscle cell chemotaxis
B	0097057	cellular_component	TRAF2-GSTP1 complex
B	0098869	biological_process	cellular oxidant detoxification
B	0110076	biological_process	negative regulation of ferroptosis
B	1901687	biological_process	glutathione derivative biosynthetic process
B	1904706	biological_process	negative regulation of vascular associated smooth muscle cell proliferation
B	1904813	cellular_component	ficolin-1-rich granule lumen
B	2001237	biological_process	negative regulation of extrinsic apoptotic signaling pathway

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	BINDING SITE FOR RESIDUE GSH A 210

Chain	Residue
A	TYR7
A	SER65
A	EAA211
A	HOH245
A	HOH308
A	HOH366
A	HOH374
B	ASP98
A	PHE8
A	ARG13
A	TRP38
A	LYS44
A	GLN51
A	LEU52
A	PRO53
A	GLN64

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EAA A 211

Chain	Residue
A	TYR7
A	PHE8
A	VAL10
A	VAL35
A	ILE104
A	GLY205
A	GSH210

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA A 212

Chain	Residue
A	TRP28
A	HOH391
A	HOH392
A	HOH425
A	HOH426
A	HOH427
B	ASP171

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 213

Chain	Residue
A	GLY77
A	GLN147
A	HOH327
A	HOH414
A	HOH415
A	HOH416

site_id	AC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA A 214

Chain	Residue
A	ASP171
A	HOH310
A	HOH407
A	HOH418
B	GLU30
B	HOH286
B	HOH364

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA A 215

Chain	Residue
A	ASP171
B	TRP28
B	HOH391
B	HOH405

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CO3 A 216

Chain	Residue
A	PHE142
A	GLN147
A	ILE148
A	ARG186
A	HOH343
A	HOH394

site_id	AC8
Number of Residues	17
Details	BINDING SITE FOR RESIDUE GSH B 210

Chain	Residue
A	ASP98
B	TYR7
B	PHE8
B	ARG13
B	TRP38
B	LYS44
B	GLN51
B	LEU52
B	PRO53
B	GLN64
B	SER65
B	EAA211
B	HOH236
B	HOH245
B	HOH274
B	HOH287
B	HOH401

site_id	AC9
Number of Residues	8
Details	BINDING SITE FOR RESIDUE EAA B 211

Chain	Residue
B	PHE8
B	VAL10
B	VAL35
B	ASN204
B	GLY205
B	GSH210
B	HOH260
B	HOH319

site_id	BC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA B 212

Chain	Residue
B	GLY77
B	GLN147
B	HOH223
B	HOH285
B	HOH348
B	HOH377
B	HOH415

site_id	BC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA B 213

Chain	Residue
A	GLU30
A	HOH434
B	ASP171
B	HOH238
B	HOH240
B	HOH295
B	HOH473

site_id	BC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CO3 B 214

Chain	Residue
B	PHE142
B	GLN147
B	ILE148
B	ARG186
B	HOH417
B	HOH469

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	158
Details	Domain: {"description":"GST N-terminal"}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	242
Details	Domain: {"description":"GST C-terminal"}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"1522586","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19396894","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19808963","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9012673","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9245401","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9351803","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9398518","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	4
Details	Modified residue: {"description":"Phosphotyrosine; by EGFR","evidences":[{"source":"PubMed","id":"19254954","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	4
Details	Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"P19157","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1oe8

Chain	Residue	Details
A	TYR7

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1oe8

Chain	Residue	Details
B	TYR7

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)