Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3H3F

Rabbit muscle L-lactate dehydrogenase in complex with NADH and oxamate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004459	molecular_function	L-lactate dehydrogenase activity
A	0005737	cellular_component	cytoplasm
A	0006089	biological_process	lactate metabolic process
A	0006090	biological_process	pyruvate metabolic process
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0019752	biological_process	carboxylic acid metabolic process
B	0003824	molecular_function	catalytic activity
B	0004459	molecular_function	L-lactate dehydrogenase activity
B	0005737	cellular_component	cytoplasm
B	0006089	biological_process	lactate metabolic process
B	0006090	biological_process	pyruvate metabolic process
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0019752	biological_process	carboxylic acid metabolic process
C	0003824	molecular_function	catalytic activity
C	0004459	molecular_function	L-lactate dehydrogenase activity
C	0005737	cellular_component	cytoplasm
C	0006089	biological_process	lactate metabolic process
C	0006090	biological_process	pyruvate metabolic process
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0019752	biological_process	carboxylic acid metabolic process
D	0003824	molecular_function	catalytic activity
D	0004459	molecular_function	L-lactate dehydrogenase activity
D	0005737	cellular_component	cytoplasm
D	0006089	biological_process	lactate metabolic process
D	0006090	biological_process	pyruvate metabolic process
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0019752	biological_process	carboxylic acid metabolic process
E	0003824	molecular_function	catalytic activity
E	0004459	molecular_function	L-lactate dehydrogenase activity
E	0005737	cellular_component	cytoplasm
E	0006089	biological_process	lactate metabolic process
E	0006090	biological_process	pyruvate metabolic process
E	0016491	molecular_function	oxidoreductase activity
E	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
E	0019752	biological_process	carboxylic acid metabolic process
F	0003824	molecular_function	catalytic activity
F	0004459	molecular_function	L-lactate dehydrogenase activity
F	0005737	cellular_component	cytoplasm
F	0006089	biological_process	lactate metabolic process
F	0006090	biological_process	pyruvate metabolic process
F	0016491	molecular_function	oxidoreductase activity
F	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
F	0019752	biological_process	carboxylic acid metabolic process
G	0003824	molecular_function	catalytic activity
G	0004459	molecular_function	L-lactate dehydrogenase activity
G	0005737	cellular_component	cytoplasm
G	0006089	biological_process	lactate metabolic process
G	0006090	biological_process	pyruvate metabolic process
G	0016491	molecular_function	oxidoreductase activity
G	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
G	0019752	biological_process	carboxylic acid metabolic process
H	0003824	molecular_function	catalytic activity
H	0004459	molecular_function	L-lactate dehydrogenase activity
H	0005737	cellular_component	cytoplasm
H	0006089	biological_process	lactate metabolic process
H	0006090	biological_process	pyruvate metabolic process
H	0016491	molecular_function	oxidoreductase activity
H	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
H	0019752	biological_process	carboxylic acid metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	22
Details	BINDING SITE FOR RESIDUE NAI A 332

Chain	Residue
A	GLY28
A	ILE115
A	VAL135
A	SER136
A	ASN137
A	HIS192
A	ILE251
A	OXM333
A	HOH335
A	HOH359
A	HOH1075
A	ALA29
A	HOH1095
A	HOH1117
E	GLY102
A	VAL30
A	ASP51
A	VAL52
A	MET53
A	THR94
A	ALA95
A	GLY96

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE OXM A 333

Chain	Residue
A	ASN137
A	ARG168
A	HIS192
A	ALA237
A	THR247
A	NAI332

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ACT A 334

Chain	Residue
A	ASP42
A	PHE70
A	ARG72

site_id	AC4
Number of Residues	26
Details	BINDING SITE FOR RESIDUE NAI B 332

Chain	Residue
B	GLY28
B	ALA29
B	VAL30
B	ASP51
B	VAL52
B	MET53
B	LYS56
B	THR94
B	ALA95
B	GLY96
B	ALA97
B	ARG98
B	VAL135
B	ASN137
B	LEU164
B	HIS192
B	THR247
B	ILE251
B	OXM333
B	HOH336
B	HOH339
B	HOH344
B	HOH347
B	HOH364
B	HOH365
B	HOH422

site_id	AC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE OXM B 333

Chain	Residue
B	GLN99
B	ARG105
B	ASN137
B	ARG168
B	HIS192
B	ALA237
B	THR247
B	NAI332

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ACT B 334

Chain	Residue
B	LYS304
B	VAL305
B	HOH392
B	HOH394

site_id	AC7
Number of Residues	28
Details	BINDING SITE FOR RESIDUE NAI C 332

Chain	Residue
C	GLY28
C	ALA29
C	VAL30
C	ASP51
C	VAL52
C	MET53
C	LYS56
C	THR94
C	ALA95
C	GLY96
C	ALA97
C	ARG98
C	ILE119
C	VAL135
C	ASN137
C	SER160
C	HIS192
C	THR247
C	ILE251
C	OXM333
C	HOH351
C	HOH352
C	HOH366
C	HOH368
C	HOH555
C	HOH729
C	HOH730
C	HOH776

site_id	AC8
Number of Residues	8
Details	BINDING SITE FOR RESIDUE OXM C 333

Chain	Residue
C	ASN137
C	ARG168
C	HIS192
C	ALA237
C	THR247
C	NAI332
C	GLN99
C	ARG105

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ACT C 334

Chain	Residue
C	ASN87
C	SER88
C	SER127
C	PRO128
C	HIS129

site_id	BC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE ACT C 335

Chain	Residue
C	LEU243

site_id	BC2
Number of Residues	20
Details	BINDING SITE FOR RESIDUE NAI D 332

Chain	Residue
D	GLY26
D	GLY28
D	ALA29
D	VAL30
D	ASP51
D	VAL52
D	MET53
D	TYR82
D	THR94
D	ALA95
D	PHE118
D	ILE119
D	VAL135
D	SER136
D	ASN137
D	VAL139
D	SER160
D	HOH567
D	HOH645
D	HOH731

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE OXM D 333

Chain	Residue
D	ASN137
D	ARG168
D	HIS192
D	ALA237

site_id	BC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE ACT D 334

Chain	Residue
D	LYS316

site_id	BC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE ACT D 335

Chain	Residue
D	GLY281
D	GLU312

site_id	BC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE ACT D 336

Chain	Residue
D	LYS316
D	ACT337

site_id	BC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ACT D 337

Chain	Residue
D	SER309
D	GLU312
D	ALA313
D	ACT336

site_id	BC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ACT D 338

Chain	Residue
D	MET40
D	ASP42
D	PHE70

site_id	BC9
Number of Residues	19
Details	BINDING SITE FOR RESIDUE NAI E 332

Chain	Residue
E	GLY28
E	ALA29
E	VAL30
E	ASP51
E	VAL52
E	MET53
E	THR94
E	ALA95
E	GLY96
E	ILE115
E	VAL135
E	ASN137
E	HIS192
E	THR247
E	ILE251
E	OXM333
E	HOH433
E	HOH568
E	HOH1054

site_id	CC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE OXM E 333

Chain	Residue
E	ASN137
E	LEU164
E	ARG168
E	HIS192
E	ALA237
E	THR247
E	NAI332

site_id	CC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ACT E 334

Chain	Residue
E	LEU182
E	HIS185
E	HOH357
E	HOH532
G	ARG170

site_id	CC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE ACT E 335

Chain	Residue
E	ASP42
E	PHE70

site_id	CC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE ACT E 336

Chain	Residue
E	ARG267
E	HOH884

site_id	CC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE ACT E 337

Chain	Residue
E	LYS242

site_id	CC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ACT E 338

Chain	Residue
E	ASP42
H	ASP42
H	ACT337

site_id	CC7
Number of Residues	29
Details	BINDING SITE FOR RESIDUE NAI F 332

Chain	Residue
B	ASN107
F	GLY28
F	ALA29
F	VAL30
F	ASP51
F	VAL52
F	MET53
F	LYS56
F	THR94
F	ALA95
F	GLY96
F	ALA97
F	ARG98
F	VAL135
F	ASN137
F	SER160
F	HIS192
F	THR247
F	ILE251
F	OXM333
F	HOH341
F	HOH343
F	HOH351
F	HOH352
F	HOH353
F	HOH390
F	HOH395
F	HOH447
F	HOH1065

site_id	CC8
Number of Residues	8
Details	BINDING SITE FOR RESIDUE OXM F 333

Chain	Residue
F	GLN99
F	ARG105
F	ASN137
F	ARG168
F	HIS192
F	ALA237
F	THR247
F	NAI332

site_id	CC9
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ACT F 334

Chain	Residue
F	MET40
F	PHE70
F	ARG72

site_id	DC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE ACT F 335

Chain	Residue
F	HIS16
F	HOH1191

site_id	DC2
Number of Residues	27
Details	BINDING SITE FOR RESIDUE NAI G 332

Chain	Residue
G	GLY28
G	ALA29
G	VAL30
G	ASP51
G	MET53
G	LYS56
G	THR94
G	ALA95
G	GLY96
G	ALA97
G	ARG98
G	ILE119
G	VAL135
G	SER136
G	ASN137
G	SER160
G	HIS192
G	THR247
G	ILE251
G	OXM333
G	HOH347
G	HOH350
G	HOH354
G	HOH367
G	HOH369
G	HOH378
G	HOH717

site_id	DC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE OXM G 333

Chain	Residue
G	GLN99
G	ARG105
G	ASN137
G	ARG168
G	HIS192
G	ALA237
G	THR247
G	NAI332

site_id	DC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ACT G 334

Chain	Residue
G	ASP42
G	PHE70
G	LEU71
G	ARG72

site_id	DC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ACT G 335

Chain	Residue
G	LEU213
G	HIS214
G	PRO215
G	LYS223
H	GLN6

site_id	DC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ACT G 336

Chain	Residue
G	LEU329
G	GLN330
G	PHE331

site_id	DC7
Number of Residues	23
Details	BINDING SITE FOR RESIDUE NAI H 332

Chain	Residue
D	GLN330
H	GLY28
H	ALA29
H	VAL30
H	ASP51
H	VAL52
H	MET53
H	THR94
H	ALA95
H	GLY96
H	ARG98
H	ILE115
H	VAL135
H	ASN137
H	HIS192
H	THR247
H	OXM333
H	HOH341
H	HOH344
H	HOH369
H	HOH372
H	HOH572
H	HOH849

site_id	DC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE OXM H 333

Chain	Residue
H	ASN137
H	ARG168
H	HIS192
H	ALA237
H	THR247
H	NAI332

site_id	DC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE ACT H 334

Chain	Residue
H	VAL305
H	HOH853

site_id	EC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE ACT H 335

Chain	Residue
H	MET40
H	ASP42

site_id	EC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE ACT H 336

Chain	Residue
H	LYS4

site_id	EC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE ACT H 337

Chain	Residue
E	ACT338
H	LYS41
H	GLU260

Functional Information from PROSITE/UniProt

site_id	PS00064
Number of Residues	7
Details	L_LDH L-lactate dehydrogenase active site. LGEHGDS

Chain	Residue	Details
A	LEU189-SER195

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Proton acceptor => ECO:0000269\|PubMed:19715328

Chain	Residue	Details
A	HIS192
B	HIS192
C	HIS192
D	HIS192
E	HIS192
F	HIS192
G	HIS192
H	HIS192

site_id	SWS_FT_FI2
Number of Residues	48
Details	BINDING:

Chain	Residue	Details
A	GLY28
A	ARG168
B	ARG105
B	ASN137
B	ARG168
B	THR247
C	GLY28
C	ARG98
C	ARG105
C	ASN137
C	ARG168
C	THR247
D	GLY28
D	ARG98
D	ARG105
D	ASN137
D	ARG168
D	THR247
E	GLY28
E	ARG98
E	ARG105
E	ASN137
E	ARG168
E	THR247
F	GLY28
F	ARG98
F	ARG105
F	ASN137
F	ARG168
F	THR247
G	GLY28
G	ARG98
G	ARG105
G	ASN137
G	ARG168
G	THR247
H	GLY28
H	ARG98
H	ARG105
H	ASN137
H	ARG168
H	THR247
A	ARG98
A	ARG105
A	ASN137
A	THR247
B	GLY28
B	ARG98

site_id	SWS_FT_FI3
Number of Residues	8
Details	MOD_RES: N-acetylalanine => ECO:0000250\|UniProtKB:P00338

Chain	Residue	Details
A	ALA1
B	ALA1
C	ALA1
D	ALA1
E	ALA1
F	ALA1
G	ALA1
H	ALA1

site_id	SWS_FT_FI4
Number of Residues	24
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250\|UniProtKB:P06151

Chain	Residue	Details
A	LYS4
A	LYS117
A	LYS317
B	LYS4
B	LYS117
B	LYS317
C	LYS4
C	LYS117
C	LYS317
D	LYS4
D	LYS117
D	LYS317
E	LYS4
E	LYS117
E	LYS317
F	LYS4
F	LYS117
F	LYS317
G	LYS4
G	LYS117
G	LYS317
H	LYS4
H	LYS117
H	LYS317

site_id	SWS_FT_FI5
Number of Residues	24
Details	MOD_RES: N6-acetyllysine => ECO:0000250\|UniProtKB:P00338

Chain	Residue	Details
C	LYS125
D	LYS13
D	LYS80
D	LYS125
E	LYS13
E	LYS80
E	LYS125
F	LYS13
F	LYS80
F	LYS125
G	LYS13
G	LYS80
G	LYS125
H	LYS13
H	LYS80
H	LYS125
A	LYS13
A	LYS80
A	LYS125
B	LYS13
B	LYS80
B	LYS125
C	LYS13
C	LYS80

site_id	SWS_FT_FI6
Number of Residues	8
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0000250\|UniProtKB:P00338

Chain	Residue	Details
A	LYS56
B	LYS56
C	LYS56
D	LYS56
E	LYS56
F	LYS56
G	LYS56
H	LYS56

site_id	SWS_FT_FI7
Number of Residues	24
Details	MOD_RES: N6-acetyllysine => ECO:0000250\|UniProtKB:P06151

Chain	Residue	Details
A	LYS223
A	LYS231
A	LYS242
B	LYS223
B	LYS231
B	LYS242
C	LYS223
C	LYS231
C	LYS242
D	LYS223
D	LYS231
D	LYS242
E	LYS223
E	LYS231
E	LYS242
F	LYS223
F	LYS231
F	LYS242
G	LYS223
G	LYS231
G	LYS242
H	LYS223
H	LYS231
H	LYS242

site_id	SWS_FT_FI8
Number of Residues	8
Details	MOD_RES: Phosphotyrosine => ECO:0000250\|UniProtKB:P06151

Chain	Residue	Details
A	TYR238
B	TYR238
C	TYR238
D	TYR238
E	TYR238
F	TYR238
G	TYR238
H	TYR238

site_id	SWS_FT_FI9
Number of Residues	16
Details	MOD_RES: Phosphothreonine => ECO:0000250\|UniProtKB:P04642

Chain	Residue	Details
A	THR308
A	THR321
B	THR308
B	THR321
C	THR308
C	THR321
D	THR308
D	THR321
E	THR308
E	THR321
F	THR308
F	THR321
G	THR308
G	THR321
H	THR308
H	THR321

site_id	SWS_FT_FI10
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:P00338

Chain	Residue	Details
A	SER309
B	SER309
C	SER309
D	SER309
E	SER309
F	SER309
G	SER309
H	SER309

site_id	SWS_FT_FI11
Number of Residues	16
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0000250\|UniProtKB:P00338

Chain	Residue	Details
A	LYS56
B	LYS56
C	LYS56
D	LYS56
E	LYS56
F	LYS56
G	LYS56
H	LYS56

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)