3GLG
Crystal Structure of a Mutant (gammaT157A) E. coli Clamp Loader Bound to Primer-Template DNA
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003689 | molecular_function | DNA clamp loader activity |
| A | 0003887 | molecular_function | DNA-directed DNA polymerase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0006260 | biological_process | DNA replication |
| A | 0006261 | biological_process | DNA-templated DNA replication |
| A | 0009360 | cellular_component | DNA polymerase III complex |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016779 | molecular_function | nucleotidyltransferase activity |
| A | 0030894 | cellular_component | replisome |
| A | 0034061 | molecular_function | DNA polymerase activity |
| A | 0043846 | cellular_component | DNA polymerase III, clamp loader complex |
| A | 0071897 | biological_process | DNA biosynthetic process |
| B | 0003677 | molecular_function | DNA binding |
| B | 0003887 | molecular_function | DNA-directed DNA polymerase activity |
| B | 0005524 | molecular_function | ATP binding |
| B | 0006260 | biological_process | DNA replication |
| B | 0009360 | cellular_component | DNA polymerase III complex |
| C | 0003677 | molecular_function | DNA binding |
| C | 0003887 | molecular_function | DNA-directed DNA polymerase activity |
| C | 0005524 | molecular_function | ATP binding |
| C | 0006260 | biological_process | DNA replication |
| C | 0009360 | cellular_component | DNA polymerase III complex |
| D | 0003677 | molecular_function | DNA binding |
| D | 0003887 | molecular_function | DNA-directed DNA polymerase activity |
| D | 0005524 | molecular_function | ATP binding |
| D | 0006260 | biological_process | DNA replication |
| D | 0009360 | cellular_component | DNA polymerase III complex |
| E | 0003677 | molecular_function | DNA binding |
| E | 0003689 | molecular_function | DNA clamp loader activity |
| E | 0003887 | molecular_function | DNA-directed DNA polymerase activity |
| E | 0005515 | molecular_function | protein binding |
| E | 0006260 | biological_process | DNA replication |
| E | 0006261 | biological_process | DNA-templated DNA replication |
| E | 0008408 | molecular_function | 3'-5' exonuclease activity |
| E | 0009360 | cellular_component | DNA polymerase III complex |
| E | 0016740 | molecular_function | transferase activity |
| E | 0016779 | molecular_function | nucleotidyltransferase activity |
| E | 0030894 | cellular_component | replisome |
| E | 0034061 | molecular_function | DNA polymerase activity |
| E | 0043846 | cellular_component | DNA polymerase III, clamp loader complex |
| E | 0071897 | biological_process | DNA biosynthetic process |
| F | 0003677 | molecular_function | DNA binding |
| F | 0003689 | molecular_function | DNA clamp loader activity |
| F | 0003887 | molecular_function | DNA-directed DNA polymerase activity |
| F | 0005515 | molecular_function | protein binding |
| F | 0006260 | biological_process | DNA replication |
| F | 0006261 | biological_process | DNA-templated DNA replication |
| F | 0009360 | cellular_component | DNA polymerase III complex |
| F | 0016740 | molecular_function | transferase activity |
| F | 0016779 | molecular_function | nucleotidyltransferase activity |
| F | 0030894 | cellular_component | replisome |
| F | 0034061 | molecular_function | DNA polymerase activity |
| F | 0043846 | cellular_component | DNA polymerase III, clamp loader complex |
| F | 0071897 | biological_process | DNA biosynthetic process |
| G | 0003677 | molecular_function | DNA binding |
| G | 0003887 | molecular_function | DNA-directed DNA polymerase activity |
| G | 0005524 | molecular_function | ATP binding |
| G | 0006260 | biological_process | DNA replication |
| G | 0009360 | cellular_component | DNA polymerase III complex |
| H | 0003677 | molecular_function | DNA binding |
| H | 0003887 | molecular_function | DNA-directed DNA polymerase activity |
| H | 0005524 | molecular_function | ATP binding |
| H | 0006260 | biological_process | DNA replication |
| H | 0009360 | cellular_component | DNA polymerase III complex |
| I | 0003677 | molecular_function | DNA binding |
| I | 0003887 | molecular_function | DNA-directed DNA polymerase activity |
| I | 0005524 | molecular_function | ATP binding |
| I | 0006260 | biological_process | DNA replication |
| I | 0009360 | cellular_component | DNA polymerase III complex |
| J | 0003677 | molecular_function | DNA binding |
| J | 0003689 | molecular_function | DNA clamp loader activity |
| J | 0003887 | molecular_function | DNA-directed DNA polymerase activity |
| J | 0005515 | molecular_function | protein binding |
| J | 0006260 | biological_process | DNA replication |
| J | 0006261 | biological_process | DNA-templated DNA replication |
| J | 0008408 | molecular_function | 3'-5' exonuclease activity |
| J | 0009360 | cellular_component | DNA polymerase III complex |
| J | 0016740 | molecular_function | transferase activity |
| J | 0016779 | molecular_function | nucleotidyltransferase activity |
| J | 0030894 | cellular_component | replisome |
| J | 0034061 | molecular_function | DNA polymerase activity |
| J | 0043846 | cellular_component | DNA polymerase III, clamp loader complex |
| J | 0071897 | biological_process | DNA biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE ADP B 400 |
| Chain | Residue |
| B | ALA7 |
| B | GLY50 |
| B | LYS51 |
| B | THR52 |
| B | SER53 |
| B | LEU214 |
| B | ARG215 |
| B | LEU218 |
| B | BEF401 |
| B | MG412 |
| C | GLU144 |
| B | TRP10 |
| B | ARG11 |
| B | PRO12 |
| B | VAL18 |
| B | VAL19 |
| B | ARG47 |
| B | GLY48 |
| B | VAL49 |
| site_id | AC2 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE BEF B 401 |
| Chain | Residue |
| B | ARG47 |
| B | GLY48 |
| B | LYS51 |
| B | GLU127 |
| B | ARG215 |
| B | ADP400 |
| B | MG412 |
| C | ARG169 |
| site_id | AC3 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE ADP C 402 |
| Chain | Residue |
| C | ALA7 |
| C | TRP10 |
| C | ARG11 |
| C | PRO12 |
| C | VAL18 |
| C | VAL19 |
| C | ARG47 |
| C | GLY48 |
| C | VAL49 |
| C | GLY50 |
| C | LYS51 |
| C | THR52 |
| C | SER53 |
| C | ARG215 |
| C | LEU218 |
| C | BEF403 |
| C | MG413 |
| D | GLU144 |
| site_id | AC4 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE BEF C 403 |
| Chain | Residue |
| C | ARG47 |
| C | LYS51 |
| C | THR52 |
| C | GLU127 |
| C | ARG215 |
| C | ADP402 |
| C | MG413 |
| D | THR165 |
| D | ARG169 |
| site_id | AC5 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE ADP D 404 |
| Chain | Residue |
| D | ALA7 |
| D | ARG11 |
| D | PRO12 |
| D | VAL18 |
| D | VAL19 |
| D | GLY48 |
| D | VAL49 |
| D | GLY50 |
| D | LYS51 |
| D | THR52 |
| D | SER53 |
| D | LEU214 |
| D | ARG215 |
| D | LEU218 |
| D | BEF405 |
| D | MG414 |
| E | GLU133 |
| site_id | AC6 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE BEF D 405 |
| Chain | Residue |
| D | ARG47 |
| D | GLY48 |
| D | LYS51 |
| D | GLU127 |
| D | ALA157 |
| D | ARG215 |
| D | ADP404 |
| D | MG414 |
| E | ARG158 |
| site_id | AC7 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE ADP G 406 |
| Chain | Residue |
| G | ALA7 |
| G | TRP10 |
| G | ARG11 |
| G | PRO12 |
| G | VAL18 |
| G | VAL19 |
| G | GLY48 |
| G | VAL49 |
| G | GLY50 |
| G | LYS51 |
| G | THR52 |
| G | SER53 |
| G | LEU214 |
| G | ARG215 |
| G | LEU218 |
| G | BEF407 |
| G | MG415 |
| H | GLU144 |
| site_id | AC8 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE BEF G 407 |
| Chain | Residue |
| G | GLY48 |
| G | LYS51 |
| G | GLU127 |
| G | ARG215 |
| G | ADP406 |
| G | MG415 |
| H | ARG169 |
| G | ARG47 |
| site_id | AC9 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE ADP H 408 |
| Chain | Residue |
| H | ALA7 |
| H | TRP10 |
| H | ARG11 |
| H | PRO12 |
| H | VAL18 |
| H | VAL19 |
| H | ARG47 |
| H | GLY48 |
| H | VAL49 |
| H | GLY50 |
| H | LYS51 |
| H | THR52 |
| H | SER53 |
| H | ARG215 |
| H | LEU218 |
| H | MG416 |
| I | GLU144 |
| I | BEF409 |
| site_id | BC1 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE BEF I 409 |
| Chain | Residue |
| H | ARG47 |
| H | LYS51 |
| H | THR52 |
| H | GLU127 |
| H | ARG215 |
| H | ADP408 |
| H | MG416 |
| I | THR165 |
| I | ARG169 |
| site_id | BC2 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE ADP I 410 |
| Chain | Residue |
| I | ALA7 |
| I | ARG11 |
| I | PRO12 |
| I | VAL18 |
| I | VAL19 |
| I | ARG47 |
| I | GLY48 |
| I | VAL49 |
| I | GLY50 |
| I | LYS51 |
| I | THR52 |
| I | SER53 |
| I | LEU214 |
| I | ARG215 |
| I | LEU218 |
| I | BEF411 |
| I | MG417 |
| J | GLU133 |
| site_id | BC3 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE BEF I 411 |
| Chain | Residue |
| I | ARG47 |
| I | GLY48 |
| I | LYS51 |
| I | GLU127 |
| I | ALA157 |
| I | ARG215 |
| I | ADP410 |
| I | MG417 |
| J | THR154 |
| J | ARG158 |
| site_id | BC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG B 412 |
| Chain | Residue |
| B | THR52 |
| B | ASP126 |
| B | GLU127 |
| B | ADP400 |
| B | BEF401 |
| site_id | BC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG C 413 |
| Chain | Residue |
| C | THR52 |
| C | ASP126 |
| C | GLU127 |
| C | ADP402 |
| C | BEF403 |
| site_id | BC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG D 414 |
| Chain | Residue |
| D | THR52 |
| D | ASP126 |
| D | GLU127 |
| D | ADP404 |
| D | BEF405 |
| site_id | BC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG G 415 |
| Chain | Residue |
| G | THR52 |
| G | ASP126 |
| G | GLU127 |
| G | ADP406 |
| G | BEF407 |
| site_id | BC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG H 416 |
| Chain | Residue |
| H | THR52 |
| H | ASP126 |
| H | GLU127 |
| H | ADP408 |
| I | BEF409 |
| site_id | BC9 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG I 417 |
| Chain | Residue |
| I | THR52 |
| I | ASP126 |
| I | GLU127 |
| I | ADP410 |
| I | BEF411 |
| J | ARG158 |
| site_id | CC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B 418 |
| Chain | Residue |
| B | CYS64 |
| B | CYS73 |
| B | CYS76 |
| B | CYS79 |
| site_id | CC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN C 419 |
| Chain | Residue |
| C | CYS64 |
| C | CYS73 |
| C | CYS76 |
| C | CYS79 |
| site_id | CC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ZN D 420 |
| Chain | Residue |
| D | CYS64 |
| D | CYS73 |
| D | GLY74 |
| D | CYS76 |
| D | CYS79 |
| site_id | CC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN E 421 |
| Chain | Residue |
| E | CYS50 |
| E | CYS59 |
| E | CYS62 |
| E | CYS65 |
| site_id | CC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN G 422 |
| Chain | Residue |
| G | CYS64 |
| G | CYS73 |
| G | CYS76 |
| G | CYS79 |
| site_id | CC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN H 423 |
| Chain | Residue |
| H | CYS64 |
| H | CYS73 |
| H | CYS76 |
| H | CYS79 |
| site_id | CC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN I 424 |
| Chain | Residue |
| I | CYS64 |
| I | CYS73 |
| I | CYS76 |
| I | CYS79 |
| site_id | CC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN J 425 |
| Chain | Residue |
| J | CYS50 |
| J | CYS59 |
| J | CYS62 |
| J | CYS65 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 278 |
| Details | Region: {"description":"Domain 1","evidences":[{"source":"PubMed","id":"11525728","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 138 |
| Details | Region: {"description":"Domain 2","evidences":[{"source":"PubMed","id":"11525728","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 42 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 24 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"11525729","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1JR3","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1g8p |
| Chain | Residue | Details |
| B | VAL253 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1g8p |
| Chain | Residue | Details |
| C | VAL253 |
| site_id | CSA3 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1g8p |
| Chain | Residue | Details |
| D | VAL253 |
| site_id | CSA4 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1g8p |
| Chain | Residue | Details |
| G | VAL253 |
| site_id | CSA5 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1g8p |
| Chain | Residue | Details |
| H | VAL253 |
| site_id | CSA6 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1g8p |
| Chain | Residue | Details |
| I | VAL253 |
