Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3FHJ

Independent saturation of three TrpRS subsites generates a partially-assembled state similar to those observed in molecular simulations

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0004812	molecular_function	aminoacyl-tRNA ligase activity
A	0004830	molecular_function	tryptophan-tRNA ligase activity
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006412	biological_process	translation
A	0006418	biological_process	tRNA aminoacylation for protein translation
A	0006436	biological_process	tryptophanyl-tRNA aminoacylation
A	0016874	molecular_function	ligase activity
B	0000166	molecular_function	nucleotide binding
B	0004812	molecular_function	aminoacyl-tRNA ligase activity
B	0004830	molecular_function	tryptophan-tRNA ligase activity
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006412	biological_process	translation
B	0006418	biological_process	tRNA aminoacylation for protein translation
B	0006436	biological_process	tryptophanyl-tRNA aminoacylation
B	0016874	molecular_function	ligase activity
C	0000166	molecular_function	nucleotide binding
C	0004812	molecular_function	aminoacyl-tRNA ligase activity
C	0004830	molecular_function	tryptophan-tRNA ligase activity
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006412	biological_process	translation
C	0006418	biological_process	tRNA aminoacylation for protein translation
C	0006436	biological_process	tryptophanyl-tRNA aminoacylation
C	0016874	molecular_function	ligase activity
D	0000166	molecular_function	nucleotide binding
D	0004812	molecular_function	aminoacyl-tRNA ligase activity
D	0004830	molecular_function	tryptophan-tRNA ligase activity
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006412	biological_process	translation
D	0006418	biological_process	tRNA aminoacylation for protein translation
D	0006436	biological_process	tryptophanyl-tRNA aminoacylation
D	0016874	molecular_function	ligase activity
E	0000166	molecular_function	nucleotide binding
E	0004812	molecular_function	aminoacyl-tRNA ligase activity
E	0004830	molecular_function	tryptophan-tRNA ligase activity
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006412	biological_process	translation
E	0006418	biological_process	tRNA aminoacylation for protein translation
E	0006436	biological_process	tryptophanyl-tRNA aminoacylation
E	0016874	molecular_function	ligase activity
F	0000166	molecular_function	nucleotide binding
F	0004812	molecular_function	aminoacyl-tRNA ligase activity
F	0004830	molecular_function	tryptophan-tRNA ligase activity
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006412	biological_process	translation
F	0006418	biological_process	tRNA aminoacylation for protein translation
F	0006436	biological_process	tryptophanyl-tRNA aminoacylation
F	0016874	molecular_function	ligase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE TRP A 1001

Chain	Residue
A	PHE5
A	GLY7
A	GLN9
A	VAL40
A	HIS43
A	MET129
A	ASP132
A	ILE133
A	AMP1003

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE TRP D 1001

Chain	Residue
D	PHE5
D	GLY7
D	HIS43
D	MET129
D	ASP132
D	GLN147
D	AMP1003

site_id	AC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE TRP B 1001

Chain	Residue
B	PHE5
B	GLY7
B	VAL40
B	HIS43
B	MET129
B	ASP132
B	ILE133
B	VAL141

site_id	AC4
Number of Residues	10
Details	BINDING SITE FOR RESIDUE TRP C 1001

Chain	Residue
C	PHE5
C	GLY7
C	GLN9
C	VAL40
C	HIS43
C	MET129
C	ASP132
C	ILE133
C	GLN147
C	AMP1003

site_id	AC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE TRP E 1001

Chain	Residue
E	GLN9
E	VAL40
E	HIS43
E	MET129
E	ASP132
E	ILE133
E	GLN147
E	AMP1003

site_id	AC6
Number of Residues	10
Details	BINDING SITE FOR RESIDUE TRP F 1001

Chain	Residue
F	PHE5
F	GLY7
F	VAL40
F	HIS43
F	MET129
F	ASP132
F	ILE133
F	VAL141
F	GLN147
F	AMP1003

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE PO4 A 1002

Chain	Residue
A	SER194
A	LYS195
A	AMP1003

site_id	AC8
Number of Residues	12
Details	BINDING SITE FOR RESIDUE AMP A 1003

Chain	Residue
A	GLY17
A	ASN18
A	GLY21
A	PRO142
A	ASP146
A	ILE176
A	ALA181
A	ILE183
A	LYS192
A	MET193
A	TRP1001
A	PO41002

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 D 1002

Chain	Residue
D	ASN18
D	LYS192
D	SER194
D	LYS195
D	AMP1003

site_id	BC1
Number of Residues	8
Details	BINDING SITE FOR RESIDUE AMP D 1003

Chain	Residue
D	GLY17
D	ASN18
D	ILE176
D	ILE183
D	LYS192
D	MET193
D	TRP1001
D	PO41002

site_id	BC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE PO4 B 1002

Chain	Residue
B	THR15
B	ASN18
B	SER194
B	LYS195
B	SER196
B	AMP1003

site_id	BC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE AMP B 1003

Chain	Residue
B	GLY17
B	ASN18
B	GLY21
B	ILE183
B	PO41002

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 C 1002

Chain	Residue
C	MET193
C	SER194
C	LYS195
C	SER196
C	AMP1003

site_id	BC5
Number of Residues	9
Details	BINDING SITE FOR RESIDUE AMP C 1003

Chain	Residue
C	ASP146
C	ILE183
C	LYS192
C	MET193
C	TRP1001
C	PO41002
C	GLY17
C	ASN18
C	GLY144

site_id	BC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PO4 E 1002

Chain	Residue
E	ASN18
E	SER194
E	LYS195
E	AMP1003

site_id	BC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE AMP E 1003

Chain	Residue
E	GLY17
E	ASN18
E	GLY21
E	ALA22
E	ILE183
E	LYS192
E	MET193
E	TRP1001
E	PO41002

site_id	BC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 F 1002

Chain	Residue
F	ASN18
F	LYS192
F	SER194
F	LYS195
F	AMP1003

site_id	BC9
Number of Residues	9
Details	BINDING SITE FOR RESIDUE AMP F 1003

Chain	Residue
F	GLY17
F	ASN18
F	GLY144
F	ILE183
F	LYS192
F	MET193
F	LYS195
F	TRP1001
F	PO41002

Functional Information from PROSITE/UniProt

site_id	PS00178
Number of Residues	10
Details	AA_TRNA_LIGASE_I Aminoacyl-transfer RNA synthetases class-I signature. P..SGvITIGNY

Chain	Residue	Details
A	PRO10-TYR19

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	24
Details	Motif: {"description":"'KMSKS' region","evidences":[{"source":"HAMAP-Rule","id":"MF_00140","evidenceCode":"ECO:0000255"},{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	58
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00140","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"26555258","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5DK4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	16
Details	Motif: {"description":"'HIGH' region","evidences":[{"source":"HAMAP-Rule","id":"MF_00140","evidenceCode":"ECO:0000255"},{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1d2r

Chain	Residue	Details
A	LYS192
A	LYS195

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1d2r

Chain	Residue	Details
D	LYS192
D	LYS195

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1d2r

Chain	Residue	Details
B	LYS192
B	LYS195

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1d2r

Chain	Residue	Details
C	LYS192
C	LYS195

site_id	CSA5
Number of Residues	2
Details	Annotated By Reference To The Literature 1d2r

Chain	Residue	Details
E	LYS192
E	LYS195

site_id	CSA6
Number of Residues	2
Details	Annotated By Reference To The Literature 1d2r

Chain	Residue	Details
F	LYS192
F	LYS195

site_id	MCSA1
Number of Residues	2
Details	M-CSA 481

Chain	Residue	Details
A	LYS192	electrostatic stabiliser
A	LYS195	electrostatic stabiliser

site_id	MCSA2
Number of Residues	2
Details	M-CSA 481

Chain	Residue	Details
B	LYS192	electrostatic stabiliser
B	LYS195	electrostatic stabiliser

site_id	MCSA3
Number of Residues	2
Details	M-CSA 481

Chain	Residue	Details
C	LYS192	electrostatic stabiliser
C	LYS195	electrostatic stabiliser

site_id	MCSA4
Number of Residues	2
Details	M-CSA 481

Chain	Residue	Details
D	LYS192	electrostatic stabiliser
D	LYS195	electrostatic stabiliser

site_id	MCSA5
Number of Residues	2
Details	M-CSA 481

Chain	Residue	Details
E	LYS192	electrostatic stabiliser
E	LYS195	electrostatic stabiliser

site_id	MCSA6
Number of Residues	2
Details	M-CSA 481

Chain	Residue	Details
F	LYS192	electrostatic stabiliser
F	LYS195	electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)