3FHJ
Independent saturation of three TrpRS subsites generates a partially-assembled state similar to those observed in molecular simulations
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0004812 | molecular_function | aminoacyl-tRNA ligase activity |
| A | 0004830 | molecular_function | tryptophan-tRNA ligase activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006412 | biological_process | translation |
| A | 0006418 | biological_process | tRNA aminoacylation for protein translation |
| A | 0006436 | biological_process | tryptophanyl-tRNA aminoacylation |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0004812 | molecular_function | aminoacyl-tRNA ligase activity |
| B | 0004830 | molecular_function | tryptophan-tRNA ligase activity |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006412 | biological_process | translation |
| B | 0006418 | biological_process | tRNA aminoacylation for protein translation |
| B | 0006436 | biological_process | tryptophanyl-tRNA aminoacylation |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0004812 | molecular_function | aminoacyl-tRNA ligase activity |
| C | 0004830 | molecular_function | tryptophan-tRNA ligase activity |
| C | 0005524 | molecular_function | ATP binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0006412 | biological_process | translation |
| C | 0006418 | biological_process | tRNA aminoacylation for protein translation |
| C | 0006436 | biological_process | tryptophanyl-tRNA aminoacylation |
| D | 0000166 | molecular_function | nucleotide binding |
| D | 0004812 | molecular_function | aminoacyl-tRNA ligase activity |
| D | 0004830 | molecular_function | tryptophan-tRNA ligase activity |
| D | 0005524 | molecular_function | ATP binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0006412 | biological_process | translation |
| D | 0006418 | biological_process | tRNA aminoacylation for protein translation |
| D | 0006436 | biological_process | tryptophanyl-tRNA aminoacylation |
| E | 0000166 | molecular_function | nucleotide binding |
| E | 0004812 | molecular_function | aminoacyl-tRNA ligase activity |
| E | 0004830 | molecular_function | tryptophan-tRNA ligase activity |
| E | 0005524 | molecular_function | ATP binding |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0006412 | biological_process | translation |
| E | 0006418 | biological_process | tRNA aminoacylation for protein translation |
| E | 0006436 | biological_process | tryptophanyl-tRNA aminoacylation |
| F | 0000166 | molecular_function | nucleotide binding |
| F | 0004812 | molecular_function | aminoacyl-tRNA ligase activity |
| F | 0004830 | molecular_function | tryptophan-tRNA ligase activity |
| F | 0005524 | molecular_function | ATP binding |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0006412 | biological_process | translation |
| F | 0006418 | biological_process | tRNA aminoacylation for protein translation |
| F | 0006436 | biological_process | tryptophanyl-tRNA aminoacylation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE TRP A 1001 |
| Chain | Residue |
| A | PHE5 |
| A | GLY7 |
| A | GLN9 |
| A | VAL40 |
| A | HIS43 |
| A | MET129 |
| A | ASP132 |
| A | ILE133 |
| A | AMP1003 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE TRP D 1001 |
| Chain | Residue |
| D | PHE5 |
| D | GLY7 |
| D | HIS43 |
| D | MET129 |
| D | ASP132 |
| D | GLN147 |
| D | AMP1003 |
| site_id | AC3 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE TRP B 1001 |
| Chain | Residue |
| B | PHE5 |
| B | GLY7 |
| B | VAL40 |
| B | HIS43 |
| B | MET129 |
| B | ASP132 |
| B | ILE133 |
| B | VAL141 |
| site_id | AC4 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE TRP C 1001 |
| Chain | Residue |
| C | PHE5 |
| C | GLY7 |
| C | GLN9 |
| C | VAL40 |
| C | HIS43 |
| C | MET129 |
| C | ASP132 |
| C | ILE133 |
| C | GLN147 |
| C | AMP1003 |
| site_id | AC5 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE TRP E 1001 |
| Chain | Residue |
| E | GLN9 |
| E | VAL40 |
| E | HIS43 |
| E | MET129 |
| E | ASP132 |
| E | ILE133 |
| E | GLN147 |
| E | AMP1003 |
| site_id | AC6 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE TRP F 1001 |
| Chain | Residue |
| F | PHE5 |
| F | GLY7 |
| F | VAL40 |
| F | HIS43 |
| F | MET129 |
| F | ASP132 |
| F | ILE133 |
| F | VAL141 |
| F | GLN147 |
| F | AMP1003 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PO4 A 1002 |
| Chain | Residue |
| A | SER194 |
| A | LYS195 |
| A | AMP1003 |
| site_id | AC8 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE AMP A 1003 |
| Chain | Residue |
| A | GLY17 |
| A | ASN18 |
| A | GLY21 |
| A | PRO142 |
| A | ASP146 |
| A | ILE176 |
| A | ALA181 |
| A | ILE183 |
| A | LYS192 |
| A | MET193 |
| A | TRP1001 |
| A | PO41002 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PO4 D 1002 |
| Chain | Residue |
| D | ASN18 |
| D | LYS192 |
| D | SER194 |
| D | LYS195 |
| D | AMP1003 |
| site_id | BC1 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE AMP D 1003 |
| Chain | Residue |
| D | GLY17 |
| D | ASN18 |
| D | ILE176 |
| D | ILE183 |
| D | LYS192 |
| D | MET193 |
| D | TRP1001 |
| D | PO41002 |
| site_id | BC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PO4 B 1002 |
| Chain | Residue |
| B | THR15 |
| B | ASN18 |
| B | SER194 |
| B | LYS195 |
| B | SER196 |
| B | AMP1003 |
| site_id | BC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE AMP B 1003 |
| Chain | Residue |
| B | GLY17 |
| B | ASN18 |
| B | GLY21 |
| B | ILE183 |
| B | PO41002 |
| site_id | BC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PO4 C 1002 |
| Chain | Residue |
| C | MET193 |
| C | SER194 |
| C | LYS195 |
| C | SER196 |
| C | AMP1003 |
| site_id | BC5 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE AMP C 1003 |
| Chain | Residue |
| C | ASP146 |
| C | ILE183 |
| C | LYS192 |
| C | MET193 |
| C | TRP1001 |
| C | PO41002 |
| C | GLY17 |
| C | ASN18 |
| C | GLY144 |
| site_id | BC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PO4 E 1002 |
| Chain | Residue |
| E | ASN18 |
| E | SER194 |
| E | LYS195 |
| E | AMP1003 |
| site_id | BC7 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE AMP E 1003 |
| Chain | Residue |
| E | GLY17 |
| E | ASN18 |
| E | GLY21 |
| E | ALA22 |
| E | ILE183 |
| E | LYS192 |
| E | MET193 |
| E | TRP1001 |
| E | PO41002 |
| site_id | BC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PO4 F 1002 |
| Chain | Residue |
| F | ASN18 |
| F | LYS192 |
| F | SER194 |
| F | LYS195 |
| F | AMP1003 |
| site_id | BC9 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE AMP F 1003 |
| Chain | Residue |
| F | GLY17 |
| F | ASN18 |
| F | GLY144 |
| F | ILE183 |
| F | LYS192 |
| F | MET193 |
| F | LYS195 |
| F | TRP1001 |
| F | PO41002 |
Functional Information from PROSITE/UniProt
| site_id | PS00178 |
| Number of Residues | 10 |
| Details | AA_TRNA_LIGASE_I Aminoacyl-transfer RNA synthetases class-I signature. P..SGvITIGNY |
| Chain | Residue | Details |
| A | PRO10-TYR19 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 36 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00140, ECO:0000269|PubMed:26555258, ECO:0007744|PDB:5DK4 |
| Chain | Residue | Details |
| A | GLN9 | |
| D | GLY144 | |
| D | ILE183 | |
| D | LYS192 | |
| B | GLN9 | |
| B | GLY17 | |
| B | ASP132 | |
| B | GLY144 | |
| B | ILE183 | |
| B | LYS192 | |
| C | GLN9 | |
| A | GLY17 | |
| C | GLY17 | |
| C | ASP132 | |
| C | GLY144 | |
| C | ILE183 | |
| C | LYS192 | |
| E | GLN9 | |
| E | GLY17 | |
| E | ASP132 | |
| E | GLY144 | |
| E | ILE183 | |
| A | ASP132 | |
| E | LYS192 | |
| F | GLN9 | |
| F | GLY17 | |
| F | ASP132 | |
| F | GLY144 | |
| F | ILE183 | |
| F | LYS192 | |
| A | GLY144 | |
| A | ILE183 | |
| A | LYS192 | |
| D | GLN9 | |
| D | GLY17 | |
| D | ASP132 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1d2r |
| Chain | Residue | Details |
| A | LYS192 | |
| A | LYS195 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1d2r |
| Chain | Residue | Details |
| D | LYS192 | |
| D | LYS195 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1d2r |
| Chain | Residue | Details |
| B | LYS192 | |
| B | LYS195 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1d2r |
| Chain | Residue | Details |
| C | LYS192 | |
| C | LYS195 |
| site_id | CSA5 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1d2r |
| Chain | Residue | Details |
| E | LYS192 | |
| E | LYS195 |
| site_id | CSA6 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1d2r |
| Chain | Residue | Details |
| F | LYS192 | |
| F | LYS195 |
| site_id | MCSA1 |
| Number of Residues | 3 |
| Details | M-CSA 481 |
| Chain | Residue | Details |
| A | LYS111 | electrostatic stabiliser |
| A | LYS192 | electrostatic stabiliser |
| A | LYS195 | electrostatic stabiliser |
| site_id | MCSA2 |
| Number of Residues | 3 |
| Details | M-CSA 481 |
| Chain | Residue | Details |
| D | LYS111 | electrostatic stabiliser |
| D | LYS192 | electrostatic stabiliser |
| D | LYS195 | electrostatic stabiliser |
| site_id | MCSA3 |
| Number of Residues | 3 |
| Details | M-CSA 481 |
| Chain | Residue | Details |
| B | LYS111 | electrostatic stabiliser |
| B | LYS192 | electrostatic stabiliser |
| B | LYS195 | electrostatic stabiliser |
| site_id | MCSA4 |
| Number of Residues | 3 |
| Details | M-CSA 481 |
| Chain | Residue | Details |
| C | LYS111 | electrostatic stabiliser |
| C | LYS192 | electrostatic stabiliser |
| C | LYS195 | electrostatic stabiliser |
| site_id | MCSA5 |
| Number of Residues | 3 |
| Details | M-CSA 481 |
| Chain | Residue | Details |
| E | LYS111 | electrostatic stabiliser |
| E | LYS192 | electrostatic stabiliser |
| E | LYS195 | electrostatic stabiliser |
| site_id | MCSA6 |
| Number of Residues | 3 |
| Details | M-CSA 481 |
| Chain | Residue | Details |
| F | LYS111 | electrostatic stabiliser |
| F | LYS192 | electrostatic stabiliser |
| F | LYS195 | electrostatic stabiliser |
