3FFZ

Domain organization in Clostridium butulinum neurotoxin type E is unique: Its implication in faster translocation

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004222	molecular_function	metalloendopeptidase activity
A	0005576	cellular_component	extracellular region
A	0006508	biological_process	proteolysis
A	0008233	molecular_function	peptidase activity
A	0008237	molecular_function	metallopeptidase activity
A	0008270	molecular_function	zinc ion binding
A	0008289	molecular_function	lipid binding
A	0008320	molecular_function	protein transmembrane transporter activity
A	0016020	cellular_component	membrane
A	0016787	molecular_function	hydrolase activity
A	0020002	cellular_component	host cell plasma membrane
A	0030430	cellular_component	host cell cytoplasm
A	0033644	cellular_component	host cell membrane
A	0035821	biological_process	modulation of process of another organism
A	0044161	cellular_component	host cell cytoplasmic vesicle
A	0044164	cellular_component	host cell cytosol
A	0044221	cellular_component	host cell synapse
A	0044231	cellular_component	host cell presynaptic membrane
A	0046872	molecular_function	metal ion binding
A	0071806	biological_process	protein transmembrane transport
A	0090729	molecular_function	toxin activity
B	0004222	molecular_function	metalloendopeptidase activity
B	0005576	cellular_component	extracellular region
B	0006508	biological_process	proteolysis
B	0008233	molecular_function	peptidase activity
B	0008237	molecular_function	metallopeptidase activity
B	0008270	molecular_function	zinc ion binding
B	0008289	molecular_function	lipid binding
B	0008320	molecular_function	protein transmembrane transporter activity
B	0016020	cellular_component	membrane
B	0016787	molecular_function	hydrolase activity
B	0020002	cellular_component	host cell plasma membrane
B	0030430	cellular_component	host cell cytoplasm
B	0033644	cellular_component	host cell membrane
B	0035821	biological_process	modulation of process of another organism
B	0044161	cellular_component	host cell cytoplasmic vesicle
B	0044164	cellular_component	host cell cytosol
B	0044221	cellular_component	host cell synapse
B	0044231	cellular_component	host cell presynaptic membrane
B	0046872	molecular_function	metal ion binding
B	0071806	biological_process	protein transmembrane transport
B	0090729	molecular_function	toxin activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN A1300

Chain	Residue
A	HIS212
A	HIS216
A	GLU251
A	HOH1289
A	HOH1318

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NA A1301

Chain	Residue
A	HOH1317
A	ALA544
A	GLU547
A	GLN548
A	LYS550

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NA A1302

Chain	Residue
A	VAL593
A	ILE596
A	ALA597
A	ILE599
A	HOH1314

---

site_id	AC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE ACT A1303

Chain	Residue
A	TYR879
A	TYR1041

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN B1300

Chain	Residue
B	HIS212
B	HIS216
B	GLU251
B	HOH1275
B	HOH1284

---

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NA B1302

Chain	Residue
B	VAL593
B	ILE596
B	ILE599
B	SER600
B	HOH1283

---

site_id	AC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE ACT B1303

Chain	Residue
B	TYR879
B	TYR881

---

Functional Information from PROSITE/UniProt

site_id	PS00142
Number of Residues	10
Details	ZINC_PROTEASE Neutral zinc metallopeptidases, zinc-binding region signature. TLMHELIHSL

Chain	Residue	Details
A	THR209-LEU218

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	98
Details	Region: {"description":"Belt","evidences":[{"source":"PubMed","id":"19118561","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	444
Details	Region: {"description":"N-terminus of receptor binding domain (N-RBD)","evidences":[{"source":"PubMed","id":"19118561","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	Motif: {"description":"Host ganglioside-binding motif","evidences":[{"source":"UniProtKB","id":"P0DPI0","evidenceCode":"ECO:0000250"},{"source":"PubMed","id":"19650874","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU10095","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15157097","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU10095","evidenceCode":"ECO:0000255"},{"source":"PDB","id":"1T3A","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1T3C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ZKW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ZKX","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ZL6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ZN3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3FFZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PDB","id":"1T3A","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1T3C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ZKW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ZKX","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ZL6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1ZN3","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3FFZ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1i1e

Chain	Residue	Details
A	ARG348
A	GLU251
A	TYR351

---

site_id	CSA2
Number of Residues	3
Details	Annotated By Reference To The Literature 1i1e

Chain	Residue	Details
B	ARG348
B	GLU251
B	TYR351

---