3F92
Crystal structure of ubiquitin-conjugating enzyme E2-25kDa (Huntington Interacting Protein 2) M172A mutant crystallized at pH 8.5
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000209 | biological_process | protein polyubiquitination |
| A | 0004842 | molecular_function | ubiquitin-protein transferase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| A | 0010800 | biological_process | positive regulation of peptidyl-threonine phosphorylation |
| A | 0010994 | biological_process | free ubiquitin chain polymerization |
| A | 0016567 | biological_process | protein ubiquitination |
| A | 0016740 | molecular_function | transferase activity |
| A | 0019787 | molecular_function | ubiquitin-like protein transferase activity |
| A | 0031625 | molecular_function | ubiquitin protein ligase binding |
| A | 0032433 | cellular_component | filopodium tip |
| A | 0032434 | biological_process | regulation of proteasomal ubiquitin-dependent protein catabolic process |
| A | 0032446 | biological_process | protein modification by small protein conjugation |
| A | 0034450 | molecular_function | ubiquitin-ubiquitin ligase activity |
| A | 0035458 | biological_process | cellular response to interferon-beta |
| A | 0060340 | biological_process | positive regulation of type I interferon-mediated signaling pathway |
| A | 0061631 | molecular_function | ubiquitin conjugating enzyme activity |
| A | 0070936 | biological_process | protein K48-linked ubiquitination |
| A | 1903265 | biological_process | positive regulation of tumor necrosis factor-mediated signaling pathway |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PG4 A 201 |
| Chain | Residue |
| A | SER185 |
| A | LYS186 |
| A | THR192 |
| site_id | AC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PG4 A 202 |
| Chain | Residue |
| A | VAL151 |
| A | ARG176 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE BME A 203 |
| Chain | Residue |
| A | CYS92 |
| A | LYS97 |
| A | ASP124 |
| A | PRO125 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE TRS A 204 |
| Chain | Residue |
| A | ASP30 |
| A | LEU31 |
| A | GLY173 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA A 205 |
| Chain | Residue |
| A | ASP0 |
| A | GLU20 |
| A | GLN99 |
| A | HOH216 |
| A | HOH265 |
Functional Information from PROSITE/UniProt
| site_id | PS00183 |
| Number of Residues | 17 |
| Details | UBC_1 Ubiquitin-conjugating (UBC) active site signature. WHPNIssvtGaICLdiL |
| Chain | Residue | Details |
| A | TRP80-LEU96 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Glycyl thioester intermediate => ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE-ProRule:PRU10133 |
| Chain | Residue | Details |
| A | CYS92 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | MOD_RES: N-acetylalanine => ECO:0000269|Ref.11, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378 |
| Chain | Residue | Details |
| A | ALA2 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | MOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| A | LYS14 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | SER159 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternate => ECO:0007744|PubMed:25114211 |
| Chain | Residue | Details |
| A | LYS14 |
