3F7T
Structure of active IspH shows a novel fold with a [3Fe-4S] cluster in the catalytic centre
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005829 | cellular_component | cytosol |
| A | 0008299 | biological_process | isoprenoid biosynthetic process |
| A | 0016114 | biological_process | terpenoid biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0019288 | biological_process | isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway |
| A | 0042380 | molecular_function | hydroxymethylbutenyl pyrophosphate reductase activity |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0050992 | biological_process | dimethylallyl diphosphate biosynthetic process |
| A | 0051538 | molecular_function | 3 iron, 4 sulfur cluster binding |
| A | 0051539 | molecular_function | 4 iron, 4 sulfur cluster binding |
| A | 0051745 | molecular_function | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0008299 | biological_process | isoprenoid biosynthetic process |
| B | 0016114 | biological_process | terpenoid biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0019288 | biological_process | isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway |
| B | 0042380 | molecular_function | hydroxymethylbutenyl pyrophosphate reductase activity |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0050992 | biological_process | dimethylallyl diphosphate biosynthetic process |
| B | 0051538 | molecular_function | 3 iron, 4 sulfur cluster binding |
| B | 0051539 | molecular_function | 4 iron, 4 sulfur cluster binding |
| B | 0051745 | molecular_function | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE F3S A 999 |
| Chain | Residue |
| A | CYS12 |
| A | GLY14 |
| A | CYS96 |
| A | THR167 |
| A | CYS197 |
| site_id | AC2 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE POP A 998 |
| Chain | Residue |
| A | THR168 |
| A | ASN224 |
| A | SER225 |
| A | SER226 |
| A | ASN227 |
| A | SER269 |
| A | HOH467 |
| A | VAL40 |
| A | HIS41 |
| A | HIS74 |
| A | HIS124 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PO4 A 901 |
| Chain | Residue |
| A | ASN7 |
| A | ARG9 |
| A | PRO292 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PO4 A 902 |
| Chain | Residue |
| A | ARG36 |
| A | GLU58 |
| A | GLN59 |
| A | ILE60 |
| A | VAL80 |
| A | GLU83 |
| A | HOH350 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE PO4 A 904 |
| Chain | Residue |
| A | ARG108 |
| A | ARG111 |
| A | ARG112 |
| site_id | AC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PO4 A 905 |
| Chain | Residue |
| A | GLN252 |
| A | GLU253 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE F3S B 999 |
| Chain | Residue |
| B | CYS12 |
| B | CYS96 |
| B | LEU98 |
| B | THR167 |
| B | CYS197 |
| B | HOH667 |
| site_id | AC8 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE POP B 998 |
| Chain | Residue |
| B | VAL40 |
| B | HIS41 |
| B | HIS74 |
| B | HIS124 |
| B | THR168 |
| B | ASN224 |
| B | SER225 |
| B | SER226 |
| B | ASN227 |
| B | SER269 |
| B | HOH397 |
| B | HOH514 |
| B | HOH667 |
| site_id | AC9 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE PO4 B 903 |
| Chain | Residue |
| A | LYS101 |
| A | PRO191 |
| B | PRO191 |
| B | ARG192 |
| B | HOH321 |
| B | HOH388 |
| B | HOH495 |
| B | HOH529 |
| B | HOH539 |
| site_id | BC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PO4 B 906 |
| Chain | Residue |
| B | ASN7 |
| B | ARG9 |
| B | PRO292 |
| B | HOH430 |
| B | HOH638 |
| site_id | BC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PO4 B 907 |
| Chain | Residue |
| B | ARG81 |
| B | LYS85 |
| site_id | BC3 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PO4 B 908 |
| Chain | Residue |
| B | ASN157 |
| B | HOH481 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor => ECO:0000305|PubMed:22137895, ECO:0000305|PubMed:22687151 |
| Chain | Residue | Details |
| A | GLU126 | |
| B | GLU126 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:20080550, ECO:0000269|PubMed:22137895, ECO:0000269|PubMed:22687151, ECO:0000269|PubMed:22948824, ECO:0000269|PubMed:23307751, ECO:0007744|PDB:3KE8, ECO:0007744|PDB:3SZL, ECO:0007744|PDB:3URK, ECO:0007744|PDB:4EB3, ECO:0007744|PDB:4H4C |
| Chain | Residue | Details |
| A | CYS12 | |
| A | CYS96 | |
| A | CYS197 | |
| B | CYS12 | |
| B | CYS96 | |
| B | CYS197 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:20080550, ECO:0000269|PubMed:22137895, ECO:0000305|PubMed:22687151, ECO:0007744|PDB:3KE8, ECO:0007744|PDB:3SZL, ECO:0007744|PDB:4EB3 |
| Chain | Residue | Details |
| A | HIS41 | |
| B | THR167 | |
| B | SER225 | |
| B | SER269 | |
| A | HIS74 | |
| A | HIS124 | |
| A | THR167 | |
| A | SER225 | |
| A | SER269 | |
| B | HIS41 | |
| B | HIS74 | |
| B | HIS124 |
