3F4V
Semi-active E176Q mutant of rice BGlu1, a plant exoglucanase/beta-glucosidase
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| A | 0004565 | molecular_function | beta-galactosidase activity |
| A | 0004567 | molecular_function | beta-mannosidase activity |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0008422 | molecular_function | beta-glucosidase activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| A | 0033907 | molecular_function | beta-D-fucosidase activity |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0042973 | molecular_function | glucan endo-1,3-beta-D-glucosidase activity |
| A | 0047668 | molecular_function | amygdalin beta-glucosidase activity |
| A | 0047701 | molecular_function | beta-L-arabinosidase activity |
| A | 0050224 | molecular_function | prunasin beta-glucosidase activity |
| A | 0080079 | molecular_function | cellobiose glucosidase activity |
| A | 0080083 | molecular_function | beta-gentiobiose beta-glucosidase activity |
| B | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| B | 0004565 | molecular_function | beta-galactosidase activity |
| B | 0004567 | molecular_function | beta-mannosidase activity |
| B | 0005576 | cellular_component | extracellular region |
| B | 0005975 | biological_process | carbohydrate metabolic process |
| B | 0008422 | molecular_function | beta-glucosidase activity |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| B | 0033907 | molecular_function | beta-D-fucosidase activity |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0042973 | molecular_function | glucan endo-1,3-beta-D-glucosidase activity |
| B | 0047668 | molecular_function | amygdalin beta-glucosidase activity |
| B | 0047701 | molecular_function | beta-L-arabinosidase activity |
| B | 0050224 | molecular_function | prunasin beta-glucosidase activity |
| B | 0080079 | molecular_function | cellobiose glucosidase activity |
| B | 0080083 | molecular_function | beta-gentiobiose beta-glucosidase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A 1001 |
| Chain | Residue |
| A | ASP65 |
| A | HIS68 |
| B | ASP65 |
| B | HIS68 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1002 |
| Chain | Residue |
| A | THR163 |
| A | ASN166 |
| A | ARG167 |
| A | HOH551 |
| A | HOH571 |
| site_id | AC3 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE MES A 1003 |
| Chain | Residue |
| A | GLY278 |
| A | HIS279 |
| A | TYR280 |
| A | GLN285 |
| A | LYS294 |
| A | PHE295 |
| A | ALA300 |
| A | HOH671 |
| A | HOH749 |
| site_id | AC4 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE GOL A 1004 |
| Chain | Residue |
| A | GLN29 |
| A | HIS130 |
| A | ASN175 |
| A | GLN176 |
| A | ASN313 |
| A | GLU386 |
| A | TRP433 |
| A | GLU440 |
| A | TRP441 |
| A | GOL1005 |
| site_id | AC5 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL A 1005 |
| Chain | Residue |
| A | GLN176 |
| A | TYR315 |
| A | TRP358 |
| A | HOH555 |
| A | HOH757 |
| A | HOH790 |
| A | GOL1004 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 B 1002 |
| Chain | Residue |
| B | THR163 |
| B | ASN166 |
| B | ARG167 |
| B | HOH508 |
| B | HOH545 |
| site_id | AC7 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE MES B 1003 |
| Chain | Residue |
| B | GLY278 |
| B | HIS279 |
| B | TYR280 |
| B | GLN285 |
| B | LYS294 |
| B | PHE295 |
| B | ALA300 |
| B | HOH572 |
| B | HOH652 |
| B | HOH883 |
| site_id | AC8 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL B 1004 |
| Chain | Residue |
| B | GLN29 |
| B | HIS130 |
| B | ASN175 |
| B | GLN176 |
| B | GLU386 |
| B | TRP433 |
| B | GLU440 |
| B | TRP441 |
| B | GOL1005 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL B 1005 |
| Chain | Residue |
| B | GLN176 |
| B | TYR315 |
| B | TRP358 |
| B | HOH498 |
| B | HOH619 |
| B | HOH759 |
| B | GOL1004 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"25252199","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"25252199","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"18308333","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"20884352","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"25252199","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"2RGM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3AHT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3F5J","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3F5K","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3F5L","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4QLK","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"20884352","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"25252199","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"3AHT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3F5J","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3F5K","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3F5L","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4QLK","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"18308333","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"20884352","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"25252199","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"2RGM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3F5K","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3F5L","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4QLK","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"18308333","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"20884352","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"25252199","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"2RGM","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3AHT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3F5K","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3F5L","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4QLK","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"Q9SPP9","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000305"},{"source":"PDB","id":"3SCW","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"Q1XH05","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 4 |
| Details | Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"source":"PROSITE-ProRule","id":"PRU00498","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1cbg |
| Chain | Residue | Details |
| A | ASN313 | |
| A | GLU386 | |
| A | GLN176 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1cbg |
| Chain | Residue | Details |
| B | ASN313 | |
| B | GLU386 | |
| B | GLN176 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1cbg |
| Chain | Residue | Details |
| A | GLU386 | |
| A | GLN176 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1cbg |
| Chain | Residue | Details |
| B | GLU386 | |
| B | GLN176 |
