3F3R

Crystal structure of yeast Thioredoxin1-glutathione mixed disulfide complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000011	biological_process	vacuole inheritance
A	0000139	cellular_component	Golgi membrane
A	0000324	cellular_component	fungal-type vacuole
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005758	cellular_component	mitochondrial intermembrane space
A	0005794	cellular_component	Golgi apparatus
A	0005829	cellular_component	cytosol
A	0006888	biological_process	endoplasmic reticulum to Golgi vesicle-mediated transport
A	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
A	0009263	biological_process	deoxyribonucleotide biosynthetic process
A	0015031	biological_process	protein transport
A	0015035	molecular_function	protein-disulfide reductase activity
A	0015036	molecular_function	disulfide oxidoreductase activity
A	0016020	cellular_component	membrane
A	0042144	biological_process	vacuole fusion, non-autophagic
A	0045454	biological_process	cell redox homeostasis
A	0120124	cellular_component	membrane fusion priming complex
B	0000011	biological_process	vacuole inheritance
B	0000139	cellular_component	Golgi membrane
B	0000324	cellular_component	fungal-type vacuole
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005758	cellular_component	mitochondrial intermembrane space
B	0005794	cellular_component	Golgi apparatus
B	0005829	cellular_component	cytosol
B	0006888	biological_process	endoplasmic reticulum to Golgi vesicle-mediated transport
B	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
B	0009263	biological_process	deoxyribonucleotide biosynthetic process
B	0015031	biological_process	protein transport
B	0015035	molecular_function	protein-disulfide reductase activity
B	0015036	molecular_function	disulfide oxidoreductase activity
B	0016020	cellular_component	membrane
B	0042144	biological_process	vacuole fusion, non-autophagic
B	0045454	biological_process	cell redox homeostasis
B	0120124	cellular_component	membrane fusion priming complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GSH A 104

Chain	Residue
A	TRP29
A	CYS30
A	PRO32
A	MET72
A	HOH156
A	HOH173
B	GLU59
B	HOH157

---

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 105

Chain	Residue
A	ALA93
A	HOH146
A	HOH170
B	THR28
B	HOH123
B	HOH136
A	PRO92

---

site_id	AC3
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GSH B 104

Chain	Residue
A	ASP58
B	TRP29
B	CYS30
B	PRO32
B	ALA71
B	MET72
B	PRO73
B	GLY89
B	ALA90
B	HOH108

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 B 105

Chain	Residue
A	THR28
A	HOH135
B	PRO92
B	ALA93
B	HOH129
B	HOH161
B	HOH168

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 106

Chain	Residue
A	SER70
B	LYS82
B	GLU83
B	HOH164

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Nucleophile

Chain	Residue	Details
A	CYS30
A	SER33
B	CYS30
B	SER33

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	SITE: Deprotonates C-terminal active site Cys

Chain	Residue	Details
A	ASP24
B	ASP24

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	SITE: Contributes to redox potential value

Chain	Residue	Details
A	GLY31
A	PRO32
B	GLY31
B	PRO32

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047

Chain	Residue	Details
A	LYS54
A	LYS66
A	LYS96
B	LYS54
B	LYS66
B	LYS96

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1cqg

Chain	Residue	Details
A	CYS30
A	SER33

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1cqg

Chain	Residue	Details
B	CYS30
B	SER33

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