3EQ3
Model of tRNA(Trp)-EF-Tu in the ribosomal pre-accommodated state revealed by cryo-EM
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| I | 0000027 | biological_process | ribosomal large subunit assembly |
| I | 0002181 | biological_process | cytoplasmic translation |
| I | 0003723 | molecular_function | RNA binding |
| I | 0003735 | molecular_function | structural constituent of ribosome |
| I | 0005515 | molecular_function | protein binding |
| I | 0005737 | cellular_component | cytoplasm |
| I | 0005829 | cellular_component | cytosol |
| I | 0005840 | cellular_component | ribosome |
| I | 0006412 | biological_process | translation |
| I | 0006415 | biological_process | translational termination |
| I | 0015968 | biological_process | stringent response |
| I | 0019843 | molecular_function | rRNA binding |
| I | 0022625 | cellular_component | cytosolic large ribosomal subunit |
| I | 0070180 | molecular_function | large ribosomal subunit rRNA binding |
| I | 1990904 | cellular_component | ribonucleoprotein complex |
| L | 0000049 | molecular_function | tRNA binding |
| L | 0000372 | biological_process | Group I intron splicing |
| L | 0002181 | biological_process | cytoplasmic translation |
| L | 0003723 | molecular_function | RNA binding |
| L | 0003735 | molecular_function | structural constituent of ribosome |
| L | 0005515 | molecular_function | protein binding |
| L | 0005737 | cellular_component | cytoplasm |
| L | 0005829 | cellular_component | cytosol |
| L | 0005840 | cellular_component | ribosome |
| L | 0006412 | biological_process | translation |
| L | 0015935 | cellular_component | small ribosomal subunit |
| L | 0019843 | molecular_function | rRNA binding |
| L | 0022627 | cellular_component | cytosolic small ribosomal subunit |
| L | 0033120 | biological_process | positive regulation of RNA splicing |
| L | 0034336 | molecular_function | misfolded RNA binding |
| L | 0034337 | biological_process | RNA folding |
| L | 0046677 | biological_process | response to antibiotic |
| L | 1990145 | biological_process | maintenance of translational fidelity |
| L | 1990904 | cellular_component | ribonucleoprotein complex |
| X | 0003746 | molecular_function | translation elongation factor activity |
| X | 0003924 | molecular_function | GTPase activity |
| X | 0005525 | molecular_function | GTP binding |
| X | 0006414 | biological_process | translational elongation |
Functional Information from PROSITE/UniProt
| site_id | PS00055 |
| Number of Residues | 8 |
| Details | RIBOSOMAL_S12 Ribosomal protein S12 signature. KkPNSAlR |
| Chain | Residue | Details |
| L | LYS42-ARG49 |
| site_id | PS00301 |
| Number of Residues | 16 |
| Details | G_TR_1 Translational (tr)-type guanine nucleotide-binding (G) domain signature. DNapeEKaRGITIntS |
| Chain | Residue | Details |
| X | ASP50-SER65 |
| site_id | PS00359 |
| Number of Residues | 16 |
| Details | RIBOSOMAL_L11 Ribosomal protein L11 signature. RsIeGTarSMGlVVeD |
| Chain | Residue | Details |
| I | ARG126-ASP141 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 194 |
| Details | Domain: {"description":"tr-type G"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 7 |
| Details | Region: {"description":"G1","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | Region: {"description":"G2","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 3 |
| Details | Region: {"description":"G3","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 3 |
| Details | Region: {"description":"G4","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Region: {"description":"G5","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 14 |
| Details | Binding site: {} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00118","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"N-acetylserine","evidences":[{"source":"PubMed","id":"6997043","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7021545","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 7 |
| Details | Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"PubMed","id":"21151122","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"N6-methyllysine; alternate","evidences":[{"source":"PubMed","id":"2022614","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"389663","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"6997043","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7021545","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"PubMed","id":"21151122","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI13 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"19150849","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"24141193","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"8416965","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI14 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"3-methylthioaspartic acid","evidences":[{"source":"PubMed","id":"8844851","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI15 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"18723842","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI16 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"N,N,N-trimethylalanine","evidences":[{"source":"PubMed","id":"7004866","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI17 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N6,N6,N6-trimethyllysine","evidences":[{"source":"PubMed","id":"7004866","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ksj |
| Chain | Residue | Details |
| X | ASP21 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ksj |
| Chain | Residue | Details |
| X | HIS84 |
